Investigation of cell-derived preparations efficacy for the treatment of severe burn wounds

Abstract

<jats:p>Aim. The aim was to estimate the efficiency of cell-containing and non-cellular dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with three different cell lines and cultural medium conditioned by these cells on the third degree burns of ICR line mice. In the control group animals were treated with fresh medium-containing gel. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing within three experimental groups treated with cell suspensions of different lines. Cell-free gels with media conditioned by these cells were shown to be effective as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells from the origin of these cells. The effectiveness of these coverages is supposed to be intermediated by biologically active substances secreted by the cells.Keywords: burn wound, dermal equivalent, stem cells, skin equivalent, skin substitute, tissue engineering.</jats:p>

From mesenchymal stem cells to their secretoms as basic components of dermal coverings for the treatment of massive burns

“…Starting from 2006 until nowadays, on the basis of experimental research data the world's scientists came to the conclusion that mesenchymal stem cells (MSCs) or multipotent mesenchymal stromal cells (MMSCs) and their derivatives, exosomes and secretomes (cultural mediums conditioned by MSCs), are really promising biologically active components for biotechnological wound coverings [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Taking this into account, it was inte res ting for us to compare the activity of human cells of different types and origin in the same experiments.…”

“…Taking this into account, it was inte res ting for us to compare the activity of human cells of different types and origin in the same experiments. We took into experimental work human cells of the established E8 line (embryonic germ cells in origin), the cells of the established 4BL line (MSCs obtained from peripheral blood of healthy donor) and the cells of the established A102 line (newborn skin fibroblasts) as the biologically active components of dermis equivalents [16][17][18].…”

“…Thus, we have provided special experiments using an animal model of burn wounds (ICR mice) for comparing the wound-healing proper ties of such biologically active components as human cells of three mentioned established lines (4BL, E8, A102) and cell-free conditioned mediums (CMs), which were produced using the cells of corresponding lines (CM-4BL, CM-E8, CM-A102) as a part of hydrogel plastic coatings [16]. It was concluded that among all six investigated dermis equivalents the most effective were those containing either MSCs of the 4BL line or their conditioned media CM-4BL.…”

“…Ensuring reliable fixation of the cell suspension on the wound surface could be achieved due to the fact that the cells will be immobilized on the surface of a filmlike wound co ve ring (for example, on polyacrylamide or collagen membranes) or distributed in a soft pharmaceutical form such as an ointment [16,[19][20][21]. In our opinion, the advantages of ointment-like substances for closing wound surfaces are:…”

“…Therefore, due to use of in vivo animal models later we decided to change slightly the direction of our research, focusing on the use of soft pharmaceutical forms of the ointment type separately from the solid carrier. The analysis of literature data led us to draw the following conclusion: it is the carbopol hydrogel based on the gelling agent Carbopol 980 with a carbopol concentration of 1.5 % that will serve as a soft pharmaceutical form in our experiments with model animals [16,[19][20][21].…”