Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia

Huët, Marie Andrea Laetitia; Wong, Li Wen; Goh, Calvin Bok Sun; Hussain, Hamed; Muzahid, Nazmul Hasan; Dwiyanto, Jacky; Lee, Shaun Wen Huey; Ayub, Qasim; Reidpath, Daniel D.; Lee, Sui Mae; Rahman, Sadequr; Tan, Joash Ban Lee

doi:10.1007/s11274-021-03083-6

Cited by 6 publications

(3 citation statements)

References 74 publications

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“…These results suggest that culture and dietary habits have the potential to impact the gut mycobiome diversity and emphasized that modern lifestyle could be associated with the alteration of the gut mycobiome α-diversity. Moreover, the effect of modern lifestyle on the gut mycobiome depends on ethnicity, which is in agreement with previous studies on the gut mycobiome from different cohorts [42,43,80].…”

Section: Discussionsupporting

confidence: 92%

Mycobiome-Host Coevolution? The Mycobiome of Ancestral Human Populations Seems to Be Different and Less Diverse Than Those of Extant Native and Urban-Industrialized Populations

et al. 2022

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Few data exist on the human gut mycobiome in relation to lifestyle, ethnicity, and dietary habits. To understand the effect of these factors on the structure of the human gut mycobiome, we analyzed sequences belonging to two extinct pre-Columbian cultures inhabiting Puerto Rico (the Huecoid and Saladoid) and compared them to coprolite samples found in Mexico and Ötzi, the Iceman’s large intestine. Stool mycobiome samples from extant populations in Peru and urban cultures from the United States were also included. The ancient Puerto Rican cultures exhibited a lower fungal diversity in comparison to the extant populations. Dissimilarity distances showed that the Huecoid gut mycobiome resembled that from ancient Mexico. Fungal genera including Aspergillus spp., Penicillium spp., Rasamsonia spp., Byssochlamys spp., Talaromyces spp., Blastomyces spp., Monascus spp., and Penicilliopsis spp. were differentially abundant in the ancient and extant populations. Despite cultural differences, certain fungal taxa were present in all samples. These results suggest that culture and diet may impact the gut mycobiome and emphasize that modern lifestyles could be associated with the alteration of gut mycobiome diversity. The present study presents data on ancient and extant human gut mycobiomes in terms of lifestyle, ethnicity, and diet in the Americas.

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Section: Discussionsupporting

confidence: 92%

Mycobiome-Host Coevolution? The Mycobiome of Ancestral Human Populations Seems to Be Different and Less Diverse Than Those of Extant Native and Urban-Industrialized Populations

et al. 2022

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“…The study was conducted in collaboration with the South East Asia Community Observatory (SEACO), a community-based research platform in Segamat District, Johor, Malaysia [26]. The human faecal samples were sourced from recruited people as described in [27]. Briefly, fresh faecal samples were collected from participants between May and August 2018.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization and comparative genomic analysis of Acinetobacter baumannii isolated from the community and the hospital: an epidemiological study in Segamat, Malaysia

et al. 2023

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Acinetobacter baumannii is a common cause of multidrug-resistant (MDR) nosocomial infections around the world. However, little is known about the persistence and dynamics of A. baumannii in a healthy community. This study investigated the role of the community as a prospective reservoir for A. baumannii and explored possible links between hospital and community isolates. A total of 12 independent A. baumannii strains were isolated from human faecal samples from the community in Segamat, Malaysia, in 2018 and 2019. Another 15 were obtained in 2020 from patients at the co-located tertiary public hospital. The antimicrobial resistance profile and biofilm formation ability were analysed, and the relatedness of community and hospital isolates was determined using whole-genome sequencing (WGS). Antibiotic profile analysis revealed that 12 out of 15 hospital isolates were MDR, but none of the community isolates were MDR. However, phylogenetic analysis based on single-nucleotide polymorphisms (SNPs) and a pangenome analysis of core genes showed clustering between four community and two hospital strains. Such clustering of strains from two different settings based on their genomes suggests that these strains could persist in both. WGS revealed 41 potential resistance genes on average in the hospital strains, but fewer (n=32) were detected in the community strains. In contrast, 68 virulence genes were commonly seen in strains from both sources. This study highlights the possible transmission threat to public health posed by virulent A. baumannii present in the gut of asymptomatic individuals in the community.

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“…The procedure used to screen for yeast, mould, and ESBL has been published 96 . Briefly, a fraction of the fresh stool specimens was enriched in buffered peptone water in a 1:9 (w/v) ratio and then cultured on their respective screening media through the spread plate technique.…”

Section: Methodsmentioning

confidence: 99%

Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia

Dwiyanto,

Huët,

Hussain

et al. 2023

npj Biofilms Microbiomes

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The prevalence of antibiotic-resistant bacteria in Southeast Asia is a significant concern, yet there is limited research on the gut resistome and its correlation with lifestyle and environmental factors in the region. This study aimed to profile the gut resistome of 200 individuals in Malaysia using shotgun metagenomic sequencing and investigate its association with questionnaire data comprising demographic and lifestyle variables. A total of 1038 antibiotic resistance genes from 26 classes were detected with a mean carriage rate of 1.74 ± 1.18 gene copies per cell per person. Correlation analysis identified 14 environmental factors, including hygiene habits, health parameters, and intestinal colonization, that were significantly associated with the resistome (adjusted multivariate PERMANOVA, p < 0.05). Notably, individuals with positive yeast cultures exhibited a reduced copy number of 15 antibiotic resistance genes. Network analysis highlighted Escherichia coli as a major resistome network hub, with a positive correlation to 36 antibiotic-resistance genes. Our findings suggest that E. coli may play a pivotal role in shaping the resistome dynamics in Segamat, Malaysia, and its abundance is strongly associated with the community’s health and lifestyle habits. Furthermore, the presence of yeast appears to be associated with the suppression of antibiotic-resistance genes.

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Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia

Cited by 6 publications

References 74 publications

Mycobiome-Host Coevolution? The Mycobiome of Ancestral Human Populations Seems to Be Different and Less Diverse Than Those of Extant Native and Urban-Industrialized Populations

Mycobiome-Host Coevolution? The Mycobiome of Ancestral Human Populations Seems to Be Different and Less Diverse Than Those of Extant Native and Urban-Industrialized Populations

Molecular characterization and comparative genomic analysis of Acinetobacter baumannii isolated from the community and the hospital: an epidemiological study in Segamat, Malaysia

Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia

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