Investigation of diazo‐derivatization of bisphenol A and its applicability for quantitation in food safety inspections using high‐performance liquid chromatography

Abstract

Bisphenol A (BPA) is a common additive in food packaging materials, and many methods have been discovered to limit BPA for the sake of food safety. This study aims to find a more sensitive quantification method for BPA. It was determined that a diazo derivative of BPA could be obtained when reacted with a stable and inexpensive reagent, p-methylaniline, exhibiting an increased response in the ultraviolet spectrum than that of nonreacted BPA. Based on this discovery, an accurate method of BPA quantitation was established using a precolumn derivatization HPLC method.The results of this study showed the low limit of detection and the limit of quantitation for BPA were 3.6 and 10.9 pg/mL on-column, respectively. This method is proven to have much higher sensitivity and a lower limit of detection than direct HPLC determination methods. This method is robust, easily handled, sensitive and cost-effective for the quantitation of BPA. In addition, the broad application of this method to determine BPA content in plastic bottled drinking water, distilled water, tap water, milk, Sprite and grape juice was demonstrated in this paper. KEYWORDS bisphenol A, diazo derivative, HPLC, migration quantity

Bisphenol A in dairy products, amount, potential risks, and the various analytical methods, a systematic review

“…In the study of Liu/2018, in the preparation of the samples, derivatization was done with p-methylaniline compound, and the percentage of recovery was reported to be 96.3 % ( Liu et al, 2019 ). In this way, stronger responses are recorded in the UV spectrum ( Liu et al, 2019 ). Also, in this study and the study of Rostamzadeh/2021, deproteinization was done with TCA.…”

“…Furthermore, bisphenol A decreases aromatase enzyme and decreases testosterone synthesis ( Preethi et al, 2014 ). This combination also leads to obesity, diabetes and infertility in men ( Liu, Li, Sun, Yang, Zheng, & Wang, 2019 ). Dermal and inhalation exposure has been reported for bisphenol A ( Mercogliano et al, 2021 ).…”

Simultaneous determination of C4-C9 alkylphenols and bisphenol A in environmental water at the Yellow River Estuary Area in China by gas chromatography-mass spectrometry

“…GC-MS is the most advanced and reliable detection technology (Subuhi et al 2020;Azzouz et al 2020). After the derivatization, the polarity and boiling point of these alkylphenols and bisphenol A decreased, the volatility and stability increased, the anti-interference ability increased, and the samples could be stored for a long time for retest (Liu et al 2019;Tan et al 2019).…”

Simultaneous Determination of C4-C9 Alkylphenols and Bisphenol a in Environmental Water Using Gas Chromatography-Mass Spectrometry Technique

“…GC-MS is also a commonly used, advanced and reliable detection technology (Subuhi et al 2020;Azzouz et al 2020). After derivatization, the nonpolarity and boiling point of APs and BPA increase, volatility and stability increase, and antiinterference ability increase, hence, the samples can be stored for a long time in case of retesting (Liu et al 2019;Tan et al 2019).…”