Investigation of Dientamoeba fragilis Prevalence and Evaluation of Sociodemographic and Clinical Features in Patients with Gastrointestinal Symptoms

Aykur, Mehmet; Kurt, Cansu Çalışkan; Erdoğan, Derya Dirim; Avcı, Çığır Biray; Vardar, Rukiye; Aydemir, Şöhret; Girginkardeşler, Nogay; Gündüz, Cumhur; Dağcı, Hande

doi:10.2478/s11686-018-00017-5

Cited by 25 publications

(22 citation statements)

References 24 publications

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“…In comparison to our results, there were also significantly higher prevalence rates (> 20 percent) in several regions of the world, including countries from Europe, Middle East, and South America [27][28][29]. The common symptoms associated with D. fragilis infection were diarrhea, abdominal pain and vomiting, in agreement with the literature [30][31][32][33]. None of the Dientamoeba-positive patients, in our study, gave history of fever or reported blood or mucous in feces.…”

Section: Discussionsupporting

confidence: 89%

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study

et al. 2020

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In most developing countries, Dientamoeba fragilis infection is an obscure protozoan infection. We aimed to determine a frequency and clinical importance of D. fragilis infection in Taif, Saudi Arabia. A 1-year case control study included patients with gastrointestinal (cases, n=114) or non-gastrointestinal symptoms (controls, n=90). The fecal samples were examined with the classical parasitological methods for intestinal protozoa, and by real time PCR for D. fragilis. The infection by D. fragilis was detected in 5.8% by PCR and in 4.4% patients by microscopy. The infection was identified more in control group (n=9) than in cases (n=3); a sole infection in 11 patients and mixed with Giardia in 1 patient. The other enteric parasites detected were Blastocystis sp. (8.3%), Giardia sp. (5.3%), Cryptosporidium sp. (2.9%), Entamoeba histolytica (1.4%), Entamoeba coli (0.9%) and Hymenolepis nana (0.4%). Our results tend to reinforce the need to increase awareness of D. fragilis infection in Saudi Arabia.

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Section: Discussionsupporting

confidence: 89%

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study

et al. 2020

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“…In this specific subset, 4.4% of the samples were positive to D. fragilis by microscopy, meaning that qPCR was at least 2-fold more sensitive than conventional microscopy (10.4 vs 4.4%) in detecting the presence of the parasite. Similarly, D. fragilis has been previously identified by microscopy in 3.3% of children with diarrhoea in Ankara (Maçin et al ., 2016), and by qPCR in 2–12% of patients with gastrointestinal symptoms in Izmir and Kayseri (Sivcan et al ., 2018; Aykur et al ., 2019). Taken together, these findings are in favour of implementing diagnostic algorithms based on PCR methods for the detection of enteric protists in clinical settings, as previously recommended (Verweij, 2014; Van Lieshout and Roestenberg, 2015).…”

Section: Discussionmentioning

confidence: 99%

Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

et al. 2020

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This epidemiological study assesses the occurrence of enteric parasites in 4303 patients attended at two public hospitals in Ankara (Turkey) during 2018–2019. Microscopy was used as a screening test. Giardia duodenalis was also identified using a commercial ELISA for the detection of parasite-specific coproantigens. Giardia-positive samples by microscopy/ELISA were confirmed by real-time PCR and characterized using a multilocus genotyping scheme. Blastocystis sp. was genotyped in a sample subset. Blastocystis sp. (11.1%, 95% CI 11.4‒14.8%) and G. duodenalis (1.56%, 95% CI 1.22‒1.96) were the most prevalent pathogens found. Cryptosporidium spp., Entamoeba histolytica and intestinal helminths were only sporadically (<0.5%) found. For G. duodenalis, sequence (n = 30) analyses revealed the presence of sub-assemblages AII (23.3%), discordant AII/AIII (23.3%) and mixed AII + AIII (6.7%) within assemblage A, and BIII (10.0%), BIV (3.3%) and discordant BIII/BIV (23.3%) within assemblage B. Two additional sequences (6.7%) were assigned to the latter assemblage but sub-assemblage information was unknown. No associations between G. duodenalis assemblages/sub-assemblages and sociodemographic and clinical variables could be demonstrated. For Blastocystis sp., sequence (n = 6) analyses identified subtypes ST1, ST2 and ST3 at equal proportions. This is the first molecular characterization of G. duodenalis based on MLG conducted in Turkey to date.

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“…D. fragilis was detected as the most common parasite species in faecal samples of studied population (27). Similarly, frequency of D. fragilis was studied in patients with GI symptoms and 23.7% of D. fragilis positives had Blastocystis infection (28). The most common protozoon was D. fragilis in Blastocystis infected individuals, 24% (53 of 221 Blastocystis infected cases).…”

Section: Discussionmentioning

confidence: 91%

The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

Malatyalı

Yıldız

Tileklioğlu

et al. 2021

Journal of Health Sciences and Medicine

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Aim: Blastocystis and Dientamoeba fragilis (D. fragilis) are among the most common protozoon species in human faecal samples. The cross-sectional studies have reported the frequencies in a variety of populations. However, we have very limited information about the co-existence rate of those protozoans. The study aimed to compare D. fragilis frequency in Blastocystis positive and negative faecal samples in order to determine the co-existence rate. The secondary objective was to analyse demographic characteristics and gastrointestinal (GI) symptoms in relation to both infections. Material and Method: In the present study, we defined a study group that included 100 Blastocystis positive faecal samples and a control group that included 100 Blastocystis negative samples. The frequency of D. fragilis in samples was determined with a PCR assay specific to the small-subunit ribosomal RNA (SS rRNA) gene. A positive control of D. fragilis was used and the samples with amplification of the expected size (863 bp) were considered as positive. In addition to the statistical comparison of frequencies, the descriptive and clinical findings of cases were analysed retrospectively with Pearson chi-square or ANOVA tests. Results: The frequency of D. fragilis was 21% in Blastocystis positive group and it was 10% in Blastocystis negative group. There was statistically significant difference in terms of D. fragilis positivity between the groups (p <0.05). Age, gender and GI symptoms did not reveal a significant difference between the following groups: only Blastocystis infected (n=77), only D. fragilis infected (n=11), infected with both protozoans (n=34) and non-infected individuals (n=89) (p >0.05). Conclusion:Our study highlighted the high co-existence of D. fragilis and Blastocystis in human faecal samples. A possible explanation for this finding may be the faecal-oral transmission of these protozoans. In addition, analysis of clinical findings was supported common asymptomatic colonisation of Blastocystis and D. fragilis.

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Investigation of Dientamoeba fragilis Prevalence and Evaluation of Sociodemographic and Clinical Features in Patients with Gastrointestinal Symptoms

Cited by 25 publications

References 24 publications

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study

Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

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