Investigation of Drug–Polymer Compatibility Using Chemometric-Assisted UV-Spectrophotometry

Abstract

A simple chemometric-assisted UV-spectrophotometric method was used to study the compatibility of clindamycin hydrochloride (HC1) with two commonly used natural controlled-release polymers, alginate (Ag) and chitosan (Ch). Standard mixtures containing 1:1, 1:2, and 1:0.5 w/w drug–polymer ratios were prepared and UV scanned. A calibration model was developed with partial least square (PLS) regression analysis for each polymer separately. Then, test mixtures containing 1:1 w/w drug–polymer ratios with different sets of drug concentrations were prepared. These were UV scanned initially and after three and seven days of storage at 25 °C. Using the calibration model, the drug recovery percent was estimated and a decrease in concentration of 10% or more from initial concentration was considered to indicate instability. PLS models with PC3 (for Ag) and PC2 (for Ch) showed a good correlation between actual and found values with root mean square error of cross validation (RMSECV) of 0.00284 and 0.01228, and calibration coefficient (R2) values of 0.996 and 0.942, respectively. The average drug recovery percent after three and seven days was 98.1 ± 2.9 and 95.4 ± 4.0 (for Ag), and 97.3 ± 2.1 and 91.4 ± 3.8 (for Ch), which suggests more drug compatibility with an Ag than a Ch polymer. Conventional techniques including DSC, XRD, FTIR, and in vitro minimum inhibitory concentration (MIC) for (1:1) drug–polymer mixtures were also performed to confirm clindamycin compatibility with Ag and Ch polymers.

Solubility Enhancement of Domperidone by Solvent Change In situ Micronization Technique

“…It is one of the most frequent spectroscopic methods for the physicochemical investigations with the view of detecting the possible interactions and compatibility between the excipients and the active drug substance. [ 28 29 ] Reducing the size of crystals and their micronization decreases the peak intensity, and also, reducing the crystallinity of the particles will make them more stable. Reduction of the particle size also increases the dissolution rate or bioavailability of microcrystals, and thus, the therapeutic action is obtained in shorter times.…”

“…DSC is an appropriate method for studying the drug's polymorphism, crystallinity, physical state changes, and the possible interactions between the drug and excipients. [ 28 ] Crystalline materials are characterized by sharp peaks, but amorphous materials have very wide peaks. [ 33 ]…”

Study on influence of polymer and surfactant on in vitro performance of biodegradable aqueous-core nanocapsules of tenofovirdisoproxil fumarate by response surface methodology

“…These pellets were scanned under diffuse reflectance mode and spectra were recorded in wave length region between 4000 cm -1 to 400 cm -1 . Every spectrum was taken as mean of 16 scans per sample (Mohamed et al, 2017). The obtained spectrum of pure drug was compared with those of the physical mixtures of the drug and polymers.…”

Effect Processing Variables on the Characteristics of Itraconazole Hollow Microspheres

“…The change in frequency and bandwidth of different interacting groups in the spectrum of the mixture of drug and excipients are studied by FTIR analysis [33]. The FT-IR spectral bands of itraconazole, ethylcellulose, Eudragit E100, placebo microspheres and optimized drug loaded formulation are shown in fig.…”