Investigation of EDTA concentration on the size of carbonated flowerlike hydroxyapatite microspheres

Yao, Shengkun; Qi, Meili; Liang, Qi; Ding, Yongling; Chen, Min; Wang, Yanmin

doi:10.1098/rsos.202148

Cited by 11 publications

(9 citation statements)

References 28 publications

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“…Qi et al synthesized HA microspheres by Na2EDTA in a hydrothermal way, with the microsphere diameter around 10 μm [16]. The concentration of Na2EDTA is significant to regulate the final morphology and high pH values contribute to the of HA microspheres [17,18]. Also in our former study, EDTA is utilized to fabricate HA microspheres in hydrothermal homogeneous precipitation method.…”

Section: Introductionmentioning

confidence: 78%

A Quick and Convenient Route to Prepare Hydroxyapatite Porous Microspheres

Liu

Liang²,

Wang³

et al. 2023

J. Phys.: Conf. Ser.

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Due to the big specific surface area, good flowability and absorption ability, porous spherical HA materials have received extensive attention of the researchers. However, the facile and convenient synthesis of well-rounded HA microspheres with a porous structure is rarely reported. What’s more, the size of HA microspheres was usually less than 10 μm. In this study, by employing hexamethylenetetramine (HMT) as the pH regulator, hydroxyapatite (HA) microspheres with good uniformity and a diameter of 30-150 μm are successfully fabricated by a one-step hydrothermal homogeneous precipitation method. To our knowledge, the synthesis of such HA microspheres by using HMT has not been reported yet. Results show that the initial pH value plays a key role in regulating the morphology of the final products and the optimal initial pH value to obtain well-rounded HA microspheres with a porous structure is 3.0-4.0, especially around 4.0. The obtained HA microspheres can be utilized as effective drug carriers for biomedical field.

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Section: Introductionmentioning

confidence: 78%

A Quick and Convenient Route to Prepare Hydroxyapatite Porous Microspheres

Liu

Liang²,

Wang³

et al. 2023

J. Phys.: Conf. Ser.

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“…Furthermore, EA used in the MPN synthesis can chelate the free Ca 2+ cations in SBF, leading to the directional growth of the CHA plates and the particular flower-like structure of HA. 42,43 The nature of crystals grown on the surface of the Meso-SiHAp@MPN pellet was investigated using EDS compared to before soaking in SBF. As shown in Figure 3E,H, KB, due to the EDS results, the calcium-to-phosphorus ratio changes from 1.48 to 2.1 from days 7 to 21 soaking in SBF, which indicates an amorphous calcium phosphate structure.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Nanocore–Shell Bone Filler Contained Mesoporous Silica Modified with Hydroxyapatite Precursors; Wrapped in a Natural Metal–Phenolic Network

Bahadorani,

Hadadzadeh,

Mirahmadi-Zare

et al. 2023

Langmuir

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Various therapeutic strategies have been developed to address bone diseases caused by aging society and skeletal defects caused by trauma or accidental events. One such approach is using bone fillers, such as hydroxyapatite (HA) and bioactive glasses. Although they have provided effective osteogenesis, infection and inflammation due to the surgical procedure and uncontrolled ion release can hinder the efficiency of bone regeneration. In response to these challenges, immobilizing a neutral metal−phenolic network on the surface of osteoconductive nanoparticles would be the master key to achieving a gradual, controlled release during the mineralization period and reducing infection and inflammation through biological pathways. In this regard, a mesoporous silica nanocomposite modified by an HA precursor was synthesized to enhance bone regeneration. In addition, to improve the therapeutic effects, its surface was wrapped with a magnesium−phenolic network made from pomegranate extract, which can simultaneously produce anti-inflammatory and antibacterial effects. The obtained core−shell nanocomposite was characterized by its physicochemical properties, biocompatibility, and bioactivity. The in vitro studies revealed that the synthesized nanocomposite exhibits higher osteogenic activity than the control groups, as confirmed by alizarin red staining. Moreover, the nanocomposite maintained low toxicity as measured by the 3-(4,5dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and increased antibacterial activity against Staphylococcus aureus and Escherichia coli compared with the control groups. Therefore, this research presents a promising strategy for bone regeneration, combining the advantages of mesoporous silica nanocomposite modified by an HA precursor with the beneficial effects of a magnesium−phenolic network.

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“…Experimental parameters: attenuated total reflection method, ZnSe crystal, spectral range 600–4000 cm –1 , resolution 2 cm –1 , 15 scans. Identification of the spectral bands (Figure S8 in the SI) was made according to published data …”

Section: Methodsmentioning

confidence: 99%

“…Identification of the spectral bands (Figure S8 in the SI) was made according to published data. 63 UV spectra were recorded using a UV-1900i spectrophotometer (Shimadzu Corp., Kyoto, Japan) in the wavelength range of 550−580 nm.…”

Section: Physico-chemicalmentioning

confidence: 99%

Crystalline Micro-Sized Carbonated Apatites: Chemical Anisotropy of the Crystallite Surfaces, Biocompatibility, Osteoconductivity, and Osteoinductive Effect Enhanced by Poly(ethylene phosphoric acid)

Nifant’ev,

Tavtorkin,

Ryndyk

et al. 2023

ACS Appl. Bio Mater.

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Carbonated hydroxyapatites (CAp) are very close to natural bone apatite in chemical composition and are regarded as a prospective bone mineral substitute for bone surgery and orthopedics. However, until now, the studies and applications of CAp were limited because of the amorphous nature of the synthetic CAp. In the present work, microsized highly crystalline carbonated apatites with uniform hexagonal (hCAp) or platelike (pCAp) morphology have been studied for the first time in vitro and in vivo, comparing against commercial hydroxyapatite (HAp) and β-tricalcuim phosphate (βTCP). In vitro experiments on dissolution of those calcium phosphate ceramics (CPCs) in acetate (pH 5.5) and Tris (pH 7.3) buffer solutions showed the following rank order of the dissolution rates: βTCP > hCAp > pCAp > HAp. The higher dissolution rate of hCAp in comparison with pCAp is explained by chemical anisotropy of the crystallite surfaces, which was proven by SEM studies of the changes in the morphology of hCAp and pCAp crystallites during hydrolysis. A 5-week experiment on subcutaneous implantation of CPC species showed the following rank order of bioresorption rates: βTCP > pCAp > hCAp > HAp. pCAp matrixes exhibited the highest biocompatibility, confirmed by histomorphological analysis. Three-month bone regeneration experiments involving a rat tibial defect model were conducted with 250–500 μm granules of pCAp and pCAp-PEPA [pCAp, pretreated with 2 wt % poly(ethylene phosphoric acid)]. Notably, pCAp-PEPA implants were resorbed at higher rates and induced the formation of more mature osseous tissue, a compact bone with Haversian systems.

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Investigation of EDTA concentration on the size of carbonated flowerlike hydroxyapatite microspheres

Cited by 11 publications

References 28 publications

A Quick and Convenient Route to Prepare Hydroxyapatite Porous Microspheres

A Quick and Convenient Route to Prepare Hydroxyapatite Porous Microspheres

Nanocore–Shell Bone Filler Contained Mesoporous Silica Modified with Hydroxyapatite Precursors; Wrapped in a Natural Metal–Phenolic Network

Crystalline Micro-Sized Carbonated Apatites: Chemical Anisotropy of the Crystallite Surfaces, Biocompatibility, Osteoconductivity, and Osteoinductive Effect Enhanced by Poly(ethylene phosphoric acid)

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