Investigation of effect of ultrasound on immobilized C. rugosa lipase: Synthesis of biomass based furfuryl derivative and green metrics evaluation study

Badgujar, Vivek C.; Badgujar, Kirtikumar C.; Yeole, Pravin M.; Bhanage, Bhalchandra M.

doi:10.1016/j.enzmictec.2020.109738

Cited by 11 publications

(8 citation statements)

References 51 publications

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“…The result of this study was consistent with the reported findings by earlier research [19,60] for immobilized Pseudomonas cepacia lipase (PCL) and CRL. The lipases had half-lives of 130 and 88 h, respectively.…”

Section: Half-lifesupporting

confidence: 93%

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Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate

et al. 2021

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A novel greener MNC/PES membrane was developed through an electrospinning technique for lipase immobilization to catalyze the synthesis of ethyl valerate (EV). In this study, the covalent immobilization of Aspergillus oryzae lipase (AOL) onto an electrospun nanofibrous membrane consisting of magnetic nanocellulose (MNC) and polyethersulfone (PES) to produce EV was statistically optimized. Raman spectroscopy, Fourier-transform infrared spectroscopy: attenuated total reflection, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, thermal gravimetric analysis (TGA), and differential thermal gravimetric (DTG) of MNC/PES-AOL demonstrated that AOL was successfully immobilized onto the fibers. The Taguchi design-assisted immobilization of AOL onto MNC/PES fibers identified that 1.10 mg/mL protein loading, 4 mL reaction volume, 250 rpm stirring rate, and 50 °C were optimal to yield 72.09% of EV in 24 h. The thermal stability of MNC/PES-AOL was improved by ≈20% over the free AOL, with reusability for up to five consecutive esterification cycles while demonstrating an exceptional half-life of 120 h. Briefly, the electrospun MNC/PES fibers that immobilized AOL showed promising applicability in yielding relatively good EV levels. This study suggests that using MNC as fillers in a PES to improve AOL activity and durability for a longer catalytic process could be a viable option.

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Section: Half-lifesupporting

confidence: 93%

“…The lipase also rates among the cheapest and most extensively utilized in various types of different reactions [16,17]. Thus, the immobilization of AOL onto compatible supports would address these concerns while also facilitating its recovery and reuse [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate

et al. 2021

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“…Furthermore, some studies have investigated the improvement in the enzyme activity of immobilized lipase with ultrasound treatment at different stages. It was discovered that ultrasound treatment at 100 W for 3 h effectively improved the activity of immobilized Candida rugosa lipase (via entrapment and cross‐linking) with a 99% yield of furfuryl ester (Badgujar et al., 2021). Nadar and Rathod (2018) first applied ultrasound (22 kHz, 11.38 W/cm 2 , 25 min) to the free lipase and then immobilized the enzyme onto a metal‐organic framework (MOF).…”

Section: Principles Of Ultrasound In Food Enzyme Activationmentioning

confidence: 99%

Sono‐activation of food enzymes: From principles to practice

Liu

Hou

2023

Comp Rev Food Sci Food Safe

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Over the last decade, sono‐activation of enzymes as an emerging research area has received considerable attention from food researchers. This kind of relatively new application of ultrasound has demonstrated promising potential in facilitating the modern food industry by broadening the application of various food enzymes, improving relevant industrial unit operation and productivity, as well as increasing the yield of target products. This review aims to provide insight into the fundamental principles and possible industrialization strategies of the sono‐activation of food enzymes to facilitate its commercialization. This review first provides an overview of ultrasound application in the activation of food protease, carbohydrase, and lipase. Then, the recent development on ultrasound activation of food enzymes is discussed on aspects including mechanisms, influencing factors, modification effects, and its applications in real food systems for free and immobilized enzymes. Despite the far fewer studies on sono‐activation of immobilized enzymes compared with those on free enzymes, we endeavored to summarize the relevant aspects in three stages: ultrasound pretreatment of free enzyme/carrier, assistance in immobilization process, and modification of the already immobilized enzyme. Lastly, challenges for the scalability of ultrasound in these target areas are discussed and future research prospects are proposed.

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“…• Increase in conversion of furfuryl alcohol from 48% to 99% in the ultrasonicated system vs. the nonsonicated set-up. • 67% energy saving in ultrasound-assisted transformation to 99% conversion vs non-sonicated system [196] Degradation of free enzyme by ultrasound is more readily observed whilst immobilisation provides greater protection and stability of the enzyme [193,194]. Ultrasound may compromise enzyme activity under excessive exposure times (>1 hour typically) and at high power due to high temperatures attained in the sonicated medium [194].…”

Section: Microalgaementioning

confidence: 99%

“…Trentin et al [195] and Badgujar et al [196] demonstrated enhanced activities in immobilized lipase under ultrasound-assisted conditions compared to conventional mixing methods. For instance, in the synthesis of furfuryl acetate from immobilised lipase Candida rugosa, the conversion of furfuryl alcohol was doubled from 48% to 99% in the ultrasonicated system at 50% duty cycle, 25 kHz frequency and 100 W power input vs. the non-sonicated set-up [196]. Increased turbulence induced by the cavitation effects of ultrasound and the associated mass transfer to and from the immobilised enzyme are highlighted as contributory factors for the observed enhancement.…”

Section: Ultrasoundmentioning

confidence: 99%

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Boodhoo

Flickinger

Woodley

et al. 2022

Chemical Engineering and Processing - Process Intensification

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Investigation of effect of ultrasound on immobilized C. rugosa lipase: Synthesis of biomass based furfuryl derivative and green metrics evaluation study

Cited by 11 publications

References 51 publications

Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate

Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate

Sono‐activation of food enzymes: From principles to practice

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

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