Investigation of extracellular polymeric substances (EPS) properties of P. aeruginosa and B. subtilis and their role in bacterial adhesion

Harimawan, Ardiyan; Ting, Yen‐Peng

doi:10.1016/j.colsurfb.2016.06.039

Cited by 114 publications

(41 citation statements)

References 55 publications

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“…EPS consists of polysaccharides, nucleic acids, lipids and polypeptides 54 and can extend up to a micron from the cell surface, 55 as found here. Our results here indicate that this material, which is also shown to aid bacterial adhesion, 53 formation of biofilms, 56 and nutrient trapping, 57 is highly negatively charged, and that SICM charge mapping is a powerful means of assessing the regions over which EPS extends. Figure 8B.…”

Section: Resultsmentioning

confidence: 64%

Scanning ion conductance microscopy reveals differences in the ionic environments of gram positive and negative bacteria

Cremin

Jones

Teahan

et al. 2020

Preprint

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This paper reports on the use of scanning ion conductance microscopy (SICM) to locally map the ionic properties and charge environment of two live bacterial strains: the gramnegative Escherichia coli and the gram-positive Bacillus subtilis. SICM results find heterogeneities across the bacterial surface, and significant differences among the grampositive and -negative bacteria. The bioelectrical environment of the B. subtilis was found to be considerably more negatively charged compared to E. coli. SICM measurements, fitted to a simplified finite element method (FEM) model, revealed surface charge values of −80 to −140 mC m−2 for the gram-negative E. coli. The gram-positive B. subtilis show a much higher conductivity around the cell wall, and surface charge values between −350 and −450 mC m−2 were found using the same simplified model. SICM was also able to detect regions of high negative charge near B. subtilis, not detected in the topographical SICM response and attributed to extracellular polymeric substance. To further explore how the B. subtilis cell wall structure can influence the SICM current response, a more comprehensive FEM model, accounting for the physical properties of the gram-positive cell wall, was developed. The new model provides a more realistic description of the cell wall and allowed investigation of the relation between its key properties and SICM currents, building foundations to further investigate and improve understanding of the gram-positive cellular microenvironment.Abstract Figure

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Section: Resultsmentioning

confidence: 64%

Scanning ion conductance microscopy reveals differences in the ionic environments of gram positive and negative bacteria

Cremin

Jones

Teahan

et al. 2020

Preprint

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“…Durante o processo de adesão e fixação celular nos minerais, os micro-organismos produzem EPS (Extracellular Polymeric Substances), os quais são biopolímeros constituídos por polissacarídeos, proteínas, glicoproteínas, glicolipídios, que determinam as propriedades dos biofilmes, pois afetam diretamente a porosidade, densidade, atividade de água, hidrofobicidade, estabilidade mecânica dos mesmos, e principalmente, a carga e as propriedades de adesão (Harimawan & Ting, 2016).…”

Section: Biolixiviação De Minérios Portadores De Terras Rarasunclassified

Desafios Para a Extração Sustentável De Minérios Portadores De Terras Raras

Souza

Nascimento

Giese

2019

HOLOS

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As Terras Raras são um grupo de elementos químicos que incluem todos os elementos do grupo dos lantanídeos, além do ítrio e escândio. Devido às suas propriedades químicas e físicas, as Terras Raras desempenham um papel essencial na indústria de produtos de alta tecnologia que tornam a disponibilidade destes elementos de suma importância para o avanço tecnológico, diretamente ligado à economia do país. As Terras Raras são encontradas em vários minerais, principalmente na monazita, na bastnasita, na xenotima e nas argilas de adsorção iônica. Devido à ocorrência do fenômeno de contração lantanídica, as Terras Raras possuem grande similaridade química e física, acarretando na dificuldade de separação e obtenção dos elementos lantanídeos na forma individual. Um dos grandes desafios da cadeia produtiva de Terras Raras é a busca por novos métodos de extração economicamente viáveis sem prejuízo do meio ambiente. Esta revisão bibliográfica pretende abordar os aspectos mineralógicos e químicos de diferentes minerais portadores de Terras Raras e discutir os desafios tecnológicos existentes nos processos de extração destes elementos, com foco no uso de tecnologias verdes para comporem a cadeia produtiva de Terras Raras.

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“…Microbes are dramatically affected by the physical and chemical properties of their microenvironment (Sheng et al, 2010;Colica et al, 2014;Harimawan and Ting, 2016). Meanwhile, microbes also have the power to alter key physical and chemical features of their microenvironment through the production of EPS.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Extracellular Polymeric Substances Amplify Water Content Variability at the Pore Scale

Guo

Furrer

Kadilak

et al. 2018

Front. Environ. Sci.

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Investigation of extracellular polymeric substances (EPS) properties of P. aeruginosa and B. subtilis and their role in bacterial adhesion

Cited by 114 publications

References 55 publications

Scanning ion conductance microscopy reveals differences in the ionic environments of gram positive and negative bacteria

Scanning ion conductance microscopy reveals differences in the ionic environments of gram positive and negative bacteria

Desafios Para a Extração Sustentável De Minérios Portadores De Terras Raras

Bacterial Extracellular Polymeric Substances Amplify Water Content Variability at the Pore Scale

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