Investigation of Formaldehyde Content in Dairy Products Available in Bangladesh by a Validated High Performance Liquid Chromatographic Method

Abstract

Highly nutritious dairy products are ingested regularly by all ages of population. Adulteration of dairy products to enhance the inherent stability and acceptability to common consumers by different harmful and toxic ingredients like formalin, an unpermitted preservative, has been a burning issue in Bangladesh over the last few years. The aim of this paper was to analyze the concentration of formaldehyde in dairy products most commonly sold in Bangladeshi markets by a validated reversed phase high performance liquid chromatographic (RP-HPLC) method to reveal the on-going alarming scenario in a scientific way. After pre-derivatization with 2,4-dinitrophenylhydrazine, formaldehyde was detected at 345 nm using a C 18 column with acetonitrile and water (45:55) as mobile phase at a flow rate of 2.0ml/min. The validated method has been applied to 41 marketed dairy products, including pasteurized milk, UHT milk, banana-mango-and chocolate-milk, flavored yoghurt, lassi, buttermilk, and skimmed milk. However, no formaldehyde was detected among the tested dairy products.

Residual formaldehyde contents in fresh white cheese in El Salvador: Seasonal changes associated with temperature

“…Regarding the two-thirds of cheese samples negative for FA found in this work, other researchers also found no traces of FA in more than 120 samples of milk and its derivatives from Bangladesh and Egypt [ Table 4 ], even though the analyzes of FA were performed with the High-Performance Liquid Chromatographic method, with detection limits lower than 0.400 mg/kg [44] and 0.020 mg/kg [45] , respectively.…”

Determination of Preservatives in Fruit Juice Products Available in Bangladesh by a Validated RP HPLC Method

“…In continuation of our research work in the field of analysis of food, beverage 34 and dairy products 35,36 here, we report a simple, robust, economic and validated method for routine analysis of preservatives as well as to determine commonly used preservatives in marketed fruit juices available in Bangladesh.…”

Novel Method of Analysis for the Determination of Residual Formaldehyde by High-Performance Liquid Chromatography

“…Other derivatisation processes have also been reported [16,43], with the use of 2,4-dinitrophenylhydrazine (DNPH) being the most widely cited technique, usually followed by analysis by HPLC-UV [11][12][13][14][44][45][46][47][48][49][50][51][52][53][54][55][56][57]. However, due to the explosive nature of DNPH and its reactivity towards oxidising reagents, commercially available DNPH in powder form contains at least 33% of water and this water content can be variable and change over time necessitating strict precautions for its storage as it can ignite if allowed to dry.…”