Investigation of Formation and Development of Anammox Biofilms by Light, Epifluorescence, and Electron Microscopy

Kallistova, A. Yu.; Nikolaev, Yu. A.; Mardanov, Andrey V.; Berestovskaya, Yu. Yu.; Грачев, В. А.; Kostrikina, N. A.; Pelevina, A. V.; Ravin, Nikolai V.; Пименов, Н. В.

doi:10.1134/s0026261720060077

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…Jettenia and Ca. Brocadia, probably occupying different ecological niches [33]. Among the nitrifiers, Nitrosomonas and Nitrospira were present, which have also been revealed in other wastewater-treating bioreactors.…”

Section: Discussionmentioning

confidence: 79%

Effect of Sulfide on the Processes of Transformation of Nitrogen Compounds and the Microbial Community Composition in the Anammox Bioreactor

Pimenov

Nikolaev

Грачев

et al. 2023

Water

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Anammox is one of the most important processes in the global nitrogen cycle and the basis for an efficient technology of nitrogen removal from wastewater. The effect of the presence of sulfide in wastewater on the transformation of nitrogen compounds by the anammox community has been insufficiently studied. The present work dealt with the effect of sulfide on nitrogen removal efficiency and the dynamics of nitrogen species in a laboratory sequencing batch bioreactor modeling the functioning of the anammox community carrying out ammonium oxidation via nitritation and anammox and nitrite oxidation. The 16S rRNA gene profiling of the community of the anammox-activated sludge attached to the stationary carrier revealed members of the key physiological groups: ammonium oxidizers of the genus Nitrosomonas, nitrite oxidizers of the genus Nitrosospira, and anammox bacteria of the genera Candidatus Brocadia and Ca. Jettenia, as well as members of other bacterial genera. Nitrate removal was not sensitive to sulfide at concentrations up to 50 mg S/L and decreased by 17% at 100 mg/L. The threshold of sulfide sensitivity for group I nitrifiers was ~50 mg/L, while anammox bacteria were resistant to sulfide concentrations of up to 100 mg S/L in the incoming water. Group II nitrifiers were probably the most sulfide-sensitive components of the community. A drastic increase in the abundance of members of the family Hydrogenophilaceae at elevated sulfide concentrations, together with the precipitation of elemental sulfur, may indicate sulfide oxidation either by molecular oxygen or via nitrate reduction; this finding requires further investigation. This is the first report on the different effects of sulfide on the growth rate of members of the nitrifying genus Nitrosomonas, increasing/decreasing or not affecting it for different phylotypes at elevated sulfide concentrations.

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Section: Discussionmentioning

confidence: 79%

Effect of Sulfide on the Processes of Transformation of Nitrogen Compounds and the Microbial Community Composition in the Anammox Bioreactor

Pimenov

Nikolaev

Грачев

et al. 2023

Water

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“…Anammox bacteria of Ca. "Jettenia, " releasing EPS in excess and enveloping cells with them, could thus protect themselves from the toxic effects of DO or other substances (Kallistova et al, 2020), which gives them an advantage over Ca. "Brocadia" and allows the spatial separation of these two genera in the same reactor.…”

Section: Discussionmentioning

confidence: 99%

“…A setup consisted of three sequencing batch reactors (SBRs) operated in parallel, working with complete biomass retention on a fibrous porous carrier (Figure 1A). A detailed design of each reactor was described in Kallistova et al (2020). Each reactor performed a single-stage partial nitritation/anammox process.…”

Section: Setup Configuration and Operationmentioning

confidence: 99%

“…The airflow was gradually increased from 20 ± 0.5 to 30 ± 0.5 l/h during the entire period of the setup operation. The medium composition and preparation are described in Kallistova et al (2020). An activated sludge containing anammox bacteria (Mardanov et al, 2019a) was used as an inoculum for the reactors' first run.…”

Section: Setup Configuration and Operationmentioning

confidence: 99%

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New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

et al. 2022

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The sensitivity of anaerobic ammonium-oxidizing (anammox) bacteria to environmental fluctuations is a frequent cause of reactor malfunctions. It was hypothesized that the addition of formate and folate would have a stimulating effect on anammox bacteria, which in turn would lead to the stability of the anammox process under conditions of a sharp increase in ammonium load, i.e., it helps overcome a stress factor. The effect of formate and folate was investigated using a setup consisting of three parallel sequencing batch reactors equipped with a carrier. Two runs of the reactors were performed. The composition of the microbial community was studied by the 16S rRNA gene profiling and metagenomic analysis. Among anammox bacteria, Ca. “Brocadia” spp. dominated during the first run. A stimulatory effect of folate on the daily nitrogen removal rate (dN) was identified. The addition of formate led to progress in dissimilatory nitrate reduction and stimulated the growth of Ca. “Jettenia” spp. The spatial separation of two anammox species was observed in the formate reactor: Ca. “Brocadia” occupied the carrier and Ca. “Jettenia”—the walls of the reactors. Biomass storage at low temperature without feeding led to an interspecies shift in anammox bacteria in favor of Ca. “Jettenia.” During the second run, a domination of Ca. “Jettenia” spp. was recorded along with a stimulating effect of formate, and there was no effect of folate on dN. A comparative genome analysis revealed the patterns suggesting different strategies used by Ca. “Brocadia” and Ca. “Jettenia” spp. to cope with environmental changes.

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Intelligent bio-assembly imaging-guided platform for real-time bacteria sterilizing and infectious therapy

et al. 2022

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Investigation of Formation and Development of Anammox Biofilms by Light, Epifluorescence, and Electron Microscopy

Cited by 8 publications

References 46 publications

Effect of Sulfide on the Processes of Transformation of Nitrogen Compounds and the Microbial Community Composition in the Anammox Bioreactor

Effect of Sulfide on the Processes of Transformation of Nitrogen Compounds and the Microbial Community Composition in the Anammox Bioreactor

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

Intelligent bio-assembly imaging-guided platform for real-time bacteria sterilizing and infectious therapy

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