Investigation of gelation mechanism of an injectable hydrogel based on chitosan by rheological measurements for a drug delivery application

Aliaghaie, Marzieh; Mirzadeh, Hamid; Dashtimoghadam, Erfan; Taranejoo, Shahrouz

doi:10.1039/c2sm25254f

Cited by 71 publications

(46 citation statements)

References 55 publications

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“…Despite several advantages offered by CS hydrogels, including ease of administration, lubricating properties or acidic pH close to vaginal pH value [17,18], hydrogels prepared with unmodified CS possess relatively low mechanical strength [19]. Modification of the CS structure by ion crosslinking enables the improvement of mechanical and rheological properties of its semisolid preparations [20]. Among various crosslinkers, β-glycerophosphate (β-GP) allows the formation of homogeneous chitosan gel in mild conditions (without gel-like precipitate) with improved organoleptic properties [21].…”

Section: Introductionmentioning

confidence: 99%

The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application

et al. 2015

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Mucoadhesive gelling systems based on chitosan and chitosan/β-glycerophosphate (β-GP) were developed in order to increase clotrimazole residence time in the vaginal cavity. Ex vivo mucoadhesiveness using porcine vaginal mucosa followed with mechanical, viscoelastic, and swelling properties of prepared hydrogels were evaluated. Drug-free, sterile, unmodified, and β-GP crosslinked chitosan were investigated for the in vitro cytotoxicity in CRL 2616 human vaginal mucosa cells using MTT assay, fluorescent microscopy, and flow cytometry analysis. Chitosan/β-GP hydrogels exhibited pseudoplastic and thixotropic properties. Ionic interaction between β-GP and chitosan improved mechanical properties of hydrogels in terms of hardness, cohesiveness, and compressibility. The hydrogels' ability to interact with porcine vaginal mucosa (measured as force of detachment and work of adhesion) was comparable to those obtained with reference mucoadhesive gel Replens™. Surprisingly, greater mucoadhesive properties were noticed for chitosan/β-GP hydrogels. The cytotoxic effect of unmodified and β-GP crosslinked chitosan was hardly affected by chitosan molecular weight, exhibited mainly through inducing apoptosis, and was found to be significantly lower in the presence of chitosan/β-GP. Furthermore, the higher amount of β-GP was used to crosslink chitosan, the lower cytotoxic effect was observed.

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Section: Introductionmentioning

confidence: 99%

The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application

et al. 2015

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“…These photos show four different states : Temperature-dependent phase transition of the chitosan/GP system was reported to be associated with several types of interactions, including ionic bridging somewhat similar to the crosslinking linkage, hydrophobic effect and hydrogen bonding [12,15,[37][38][39][40]. Since these interactions coexist with different intensities in the chitosan/GP system during phase transformation, finding the exact mechanism of gelation for the chitosan/GP system still remains an open-ended scientific challenge [12,42]. Despite complexity in phase transition mechanism, several attempts have been made to figure out the details for the thermosensitive behavior of the chitosan/GP gel [15,37,39,41,42].…”

Section: Basic Parameters Of Dex-plasmentioning

confidence: 97%

Injectable chitosan/dextran-polylactide/glycerophosphate hydrogels and their biodegradation

Zhou

Liu

et al. 2015

Polymer Degradation and Stability

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“…To further improve stability as well as to achieve a mucoadhesive formulation, the surface of P90H/P50 liposomes was coated with CH. Successively, the polymer was crosslinked with GP to obtain the GP/CH microcomplexes, by the ionic interactions between positively charged amino groups of chitosan and negatively charged GP molecules (29,40). The crosslinkage treatment of chitosan coating may improve carrier stability and ability to control drug delivery.…”

Section: Preparation and Characterizationmentioning

confidence: 99%

“…Therefore, in the present study, β-glycerolphosphate (GP), a non-toxic and biocompatible molecule, was used to crosslink the CH by ionotropic gelation. The crosslinkage of CH-coated liposomes leads to formation of GP/CH microcomplexes: single liposomes or vesicle clusters are enveloped by the GP/CH coating (27)(28)(29). Noteworthy, the experimental setting did not require the use of organic solvents, thus, reducing the risk of toxicity due to residual solvents in the final formulation.…”

Section: Introductionmentioning

confidence: 99%

Improving Oral Bioavailability and Pharmacokinetics of Liposomal Metformin by Glycerolphosphate–Chitosan Microcomplexation

et al. 2013

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Abstract. The purpose of this study was to develop a new delivery system capable of improving bioavailability and controlling release of hydrophilic drugs. Metformin-loaded liposomes were prepared and to improve their stability surface was coated with chitosan cross-linked with the biocompatible β-glycerolphosphate. X-ray diffraction, differential scanning calorimetry, as well as rheological analysis were performed to investigate interactions between chitosan and β-glycerolphosphate molecules. The entrapment of liposomes into the chitosan-β-glycerolphosphate network was assessed by scanning electron microscopy and transmission electron microscopy. Swelling and mucoadhesive properties as well as drug release were evaluated in vitro while the drug oral bioavailability was evaluated in vivo on Wistar rats. Results clearly showed that, compared to control, the proposed microcomplexes led to a 2.5-fold increase of metformin T max with a 40% augmentation of the AUC/D value.

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Investigation of gelation mechanism of an injectable hydrogel based on chitosan by rheological measurements for a drug delivery application

Cited by 71 publications

References 55 publications

The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application

The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application

Injectable chitosan/dextran-polylactide/glycerophosphate hydrogels and their biodegradation

Improving Oral Bioavailability and Pharmacokinetics of Liposomal Metformin by Glycerolphosphate–Chitosan Microcomplexation

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