Investigation of immunofluorescence cross-reactions against Trichinella spiralis by western blot (immunoblot) analysis

Robert, F.; Weil, B; Kassis, Nadim; Dupouy‐Camet, Jean

doi:10.1128/cdli.3.5.575-577.1996

Cited by 30 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…WB has been recommended for confirmation of Trichinella infection in ELISA-positive sera as a common profile for trichinellosis distinct from other infections have been established when panels of Trichinella-positive sera are compared (Frey et al, 2009;Gómez-Morales et al, 2012;Robert et al, 1996). In this study, only a small panel of experimentally-infected (n=4) and naturally-infected (n=1) sera could be used to establish the Trichinella profile.…”

Section: Investigation Of Serum From Wild Boar In a High-risk Regionmentioning

confidence: 99%

Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)

Cuttell

Morales

Cookson³

et al. 2014

Veterinary Parasitology

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 35A c c e p t e d M a n u s c r i p t Trichinella introduction within mainland Australia, which is considered Trichinella-free. Sera were 35 examined using both an 'in-house' and a commercially available indirect-ELISA that used other parasitic infections, more work using well-defined cohorts of positive and negative samples is 53 required. Even if the specificity of the ELISAs is proven to be low, their ability to correctly classify 54 the small number of true positive sera in this study indicates utility in screening wild boar 55 populations for reactive sera which can be followed up with additional testing.56 57

show abstract

Section: Investigation Of Serum From Wild Boar In a High-risk Regionmentioning

confidence: 99%

Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)

Cuttell

Morales

Cookson³

et al. 2014

Veterinary Parasitology

View full text Add to dashboard Cite

show abstract

“…There is an obvious window period of 3–4 weeks between Trichinella infection and specific antibody positivity. The indirect immunofluorescence test (IIF) with frozen sections of infected tissue or isolated whole ML as antigens could detect specific antibodies in sera of experimentally infected mice 1–2 weeks after infection or sera of patients with trichinellosis from day 6 after the onset of illness [ 17 , 18 ], but cross-reactions with Trichinella antigens were observed in the patients with autoimmune diseases and other helminthiases because IIF is based on cuticle surface antigens of ML [ 19 ]. This is particularly important in regions where other human helminthiases (e.g., ascariasis, trichuriasis, clonorchiasis, paragonimiasis, cysticercosis and so on) are common and cross-reactions with these parasites could give could give false positive results [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

New insights on serodiagnosis of trichinellosis during window period: early diagnostic antigens from Trichinella spiralis intestinal worms

et al. 2017

View full text Add to dashboard Cite

The clinical diagnosis of trichinellosis is difficult because its clinical manifestations are nonspecific. Detection of anti-Trichinella IgG by ELISA using T. spiralis muscle larval excretory-secretory (ES) antigens is the most commonly used serological method for diagnosis of trichinellosis, but the main disadvantage is false negativity during the early stage of infection. There is an obvious window period between Trichinella infection and antibody positivity.During the intestinal stage of Trichinella infection, the ES antigens of intestinal worms (intestinal infective larvae and adults) are exposed to host’s immune system at the earliest time and elicit the production of specific anti-Trichinella antibodies. Anti-Trichinella IgG antibodies in infected mice were detectable by ELISA with ES antigens of intestinal worms as soon as 8–10 days post infection (dpi), but ELISA with muscle larval ES antigens did not permit detection of infected mice before 12 dpi. Therefore, the new early antigens from T. spiralis intestinal worms should be screened, identified and characterized for early serodiagnosis of trichinellosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s40249-017-0252-z) contains supplementary material, which is available to authorized users.

show abstract

“…The ELISA test, due to its high sensitivity, is most commonly used to detect IgG against Trichinella (Dupouy-Camet & Bruschi, 2007, Dupouy-Camet et al, 2002. After the ELISA test, seropositive sera are submitted to specialized laboratories that use the Wb confirmatory test to confirm previous test results (Gamble et al, 2004., Pozio et al, 2003, Robert et al, 1996.…”

Section: Discussionmentioning

confidence: 99%

Serological diagnosis of trichinellosis in patients in Southern and Eastern Serbia in the period from 2007 to 2018

Miladinović-Tasić

Ignjatović

2019

VET GLASNIK

View full text Add to dashboard Cite

Introduction. The Nisava District has had low prevalence and incidence rates of trichinellosis for a long period. The aims of this study were to present the frequency of occurrence of trichinellosis in Nisava District and other districts in Southern and Eastern Serbia during 2007-2018, to investigate the demographic characteristics in the region and to compare the tests used for serological diagnosis of trichinellosis. Materials and Methods. This was a retrospective epidemiological study in which we analyzed the results of serological analyses of 139 serum samples taken from individual patients suspected to be infected with Trichinella spp. Indirect immunofluorescence (IIF) and immunoenzyme (ELISA) methods were used for serological diagnosis of trichinellosis. Results and Conclusions. Of 139 examined sera, 36 (25.9%) were seropositive. Most seropositive findings were encountered in 2012 and 2014 (in both these years, 12 sera were positive, i.e. 33.3% of the total 36 positive sera), when three minor outbreaks of trichinellosis were reported. Seropositivity was equally distributed according to patient sex and according to the district where they reported with illness. Positive serological findings were statistically significantly more common in hospitalized patients than in out-patients. Comparison of the findings with both IIF and ELISA test established the performance level of the tests, with the ELISA test being less sensitive than the IIF test. Immunodiagnostic procedures for the diagnosis of trichinellosis must be improved in view of the sporadic cases and minor outbreaks of the disease in the studied districts of Eastern and Southeastern Serbia.

show abstract

Investigation of immunofluorescence cross-reactions against Trichinella spiralis by western blot (immunoblot) analysis

Cited by 30 publications

References 17 publications

Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)

Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)

New insights on serodiagnosis of trichinellosis during window period: early diagnostic antigens from Trichinella spiralis intestinal worms

Serological diagnosis of trichinellosis in patients in Southern and Eastern Serbia in the period from 2007 to 2018

Contact Info

Product

Resources

About