Investigation of in vitro PDT activities of zinc phthalocyanine immobilised TiO 2 nanoparticles

Photodynamic therapy and nuclear imaging activities of zinc phthalocyanine‐integrated TiO2 nanoparticles in breast and cervical tumors

“…R f (relative front) values of Na 131 I, oxidized 131 I, 131 I‐ZnPc, and 131 I‐ZnPc‐TiO 2 were determined as 0.45, 0.93, 0.95, and 0.97 for mobile phase1; 0.05, 0.05, 0.93, and 0.96 for mobile phase 2, respectively. According to the TLRC results, radiolabeling yields of ZnPc and ZnPc‐TiO 2 were defined as 91.9 ± 3.1 and 91.2 ± 4.3%, respectively …”

“…Briefly, the radiolabeling of ZnPc/ZnPc‐TiO 2 was performed at optimum conditions (pH 7, 1.00 mg iodogen and 45 min of reaction time for ZnPc; pH 5, 1.00 mg iodogen and 60 min of reaction time for ZnPc‐TiO 2 ), described by Yurt et al . The stock solution of the compounds was prepared by ZnPc/ZnPc‐TiO 2 (1 mg) dissolved in 300 μl DMSO.…”

“…The cellulose sheets were scanned on a TLC scanner (BioScan AR‐2000 Washington DC, USA) for determining the amount of radiolabeling yield and the R f values of molecules. Two different mobile phases used in radiolabeling studies [mobile phase 1: n ‐butanol‐water‐acetic acid (4‐2‐1) and mobile phase 2: chloroform‐acetic acid (9‐1)] …”

“…The photodynamic therapy efficacies of ZnPc, ZnPc‐TiO 2 , and TiO 2 have been evaluated in control group that non‐cytotoxicity at concentrations of 1.57, 3.13, and 6.25 μ m without phototherapy (PT‐). The phototoxicities of ZnPc, ZnPc‐TiO 2 , and TiO 2 were determined according to the untreated control group in which the substance was not applied and the light was applied …”

“…Therefore, it is expected that TiO 2 nanoparticles will increase the accumulation of compound in tumor tissue. They reach a deeper tumor tissue because of the absorption of the Pc in the visible region thus increase the PDT yield . Lopez et al.…”