In recent years, Higher Education Institutes (HEIs) worldwide have invested heavily in the adoption of e-learning with a view to improving the quality of their education. However, the success of their development faced numerous challenges, particularly, the end user's acceptance, including the perceptions, and engagement. Therefore, surveying lecturers' attitudes toward e-learning in LHEIs is considered to be a meaningful stage before thoroughly embarking e-learning practices in educational activities. Instructors' attitudes towards the use of e-learning have been extensively investigated in the educational system worldwide, however, in Libya, this research is ineffective and limited. In light of this, the present study aims to investigate the perception of instructors' in LHEIs and their attitudes towards implementing e-learning technology, where an online questionnaire has been utilized to collect data from 219 academic staff in various faculties at Misurata University (MU), in Libya. The collected data were analyzed using Statistical Package for Social sciences (SPSS) to investigate the impact of demographic characteristics of participants on their attitude towards e-learning. The study concluded with a set of findings that revealed that the demographic characteristics of participants might have a significant impact on their attitude towards e-learning; which may directly impact the success of e-learning adoption in higher education. The results also show that the instructors in LHEIs have a positive attitude towards elearning.