Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans

Abstract

Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.

Salicylic acid‐enhanced biosynthesis of pharmacologically important lignans and neo lignans in cell suspension culture of Linum ussitatsimum L

“…The biosynthesis of these BAI's in wild grown plants is governed by several environmental factors . To overcome these issues, an alternative platform of plant in vitro culture technology was established and evaluated .…”

Small-Scale Bioreactor for Sterile Hydroponics and Hairy Roots: Metabolic Diversity and Salicylic Acid Exudation by Hairy Roots of Hyoscyamus niger

“…Hairy roots also proved useful in studies of interactions between crop plants and herbivores [18]. Biomass production in hairy root cultures is higher than in callus or suspension cultures, therefore hairy roots have been exploited for the production of plant secondary metabolites [12,[19][20][21][22]. This strategy appears particularly promising for large-scale production of metabolites that are primarily synthesized in the roots, such as certain tropane alkaloids of medical interest [23] and glucosinolates with chemoprotective potential [24].…”

“…Optimization of the production system requires comparing multiple genetically enhanced lines because the efficiency of promoters used to drive heterologous gene expression varies among transformation events and even among different parts of the same plant [33]. Similarly, the expression of target genes and the yield of the desired products varies among hairy root lines [21,28]. Production of natural products by plants that have not been genetically engineered requires testing multiple accessions, too, because secondary metabolite synthesis varies among plant accessions in a way that is often not predictable based on the genotype [34,35].…”

“…Production of natural products by plants that have not been genetically engineered requires testing multiple accessions, too, because secondary metabolite synthesis varies among plant accessions in a way that is often not predictable based on the genotype [34,35]. Furthermore, cultivation conditions strongly affect production of plant secondary metabolites in hydroponics [36] as well as in hairy root cultures [21,37]. For these reasons, multiple cultures have to be grown in parallel during the optimization of hairy root-based production systems.…”

Bioactive Phenolics and Polyphenols: Current Advances and Future Trends

“…Twenty-six contributions (19 research and seven review articles) in this Special Issue show some of the current advances in bioactive phenolics and polyphenols. Research articles published were mainly focused on the evaluation of different bioactivities of phenolics, with an emphasis on the prevention of chronic diseases, whereas another important number of papers described methods for the production of phenolics by chemoenzymatic preparations [ 3 ], hairy root culture bioreactors [ 4 ], and elicitation of the secondary plant metabolism by preharvest [ 5 ] and postharvest [ 6 ] abiotic stresses.…”