Investigation of lipid modifications in J774 macrophages by vibrational spectroscopies after eicosapentaenoic acid membrane incorporation in unloaded and cholesterol-loaded cells

Tfaili, Sana; Assaad, Almar Al; Fournier, Natalie; Allaoui, Fatima; Paul, Jean–Louis; Chaminade, Pierre; Tfayli, Ali

doi:10.1016/j.talanta.2019.01.122

Cited by 6 publications

(1 citation statement)

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“…The spectrum delivered by Raman acoustofluidics shows prominent peaks in the 900–1400 cm −1 band, which are smoothed out in the mentioned reference. According to Tfaili et al, [ 51 ] some Raman bands are described as follows. The band around 1240 cm −1 corresponds to random coil amide III, while at 1371 cm −1 , the peak relates to δ (CH 2 ) lipids, adenine, and cytosine in DNA/RNA.…”

Section: Resultsmentioning

confidence: 99%

3D‐Printed Acoustofluidic Devices for Raman Spectroscopy of Cells

Santos

Silva

et al. 2021

Adv Eng Mater

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Acoustofluidics technology can be used to trap live cells (and also micro/nanoparticles) in microenvironments suitable for cell assays. Herein, a cheap and easyto-fabricate device is proposed that works with Raman spectroscopy for biosensing applications. The device comprises a 3D-printed microchamber working as a halfwavelength acoustic resonator. By tuning the resonance frequency with a low voltage (%4 V), cells or particles are aggregated and levitated in seconds by the action of the acoustic radiation force. Based on finite element simulations, the radiation force field produced inside the device is described. In the cellular enrichment (aggregation) process, a metastable honeycomb lattice is formed mostly due to the cell-to-cell attraction caused by the secondary acoustic radiation force. Orderly and metastable levitating aggregates provide an excellent arrangement for Raman spectroscopy to investigate cells individually. Polystyrene particles are used for the device characterization and Raman acquisition process. Biosensing applications are showcased with live murine macrophages J774.A1, which are used in infection assay of leishmaniasis disease. The unique features of the device, e.g., simple fabrication process with cheap materials, simple operation, fast time response, and formation of metastable cellular aggregates; hold a noteworthy potential for applications in life sciences and biotechnology involving cell assays.

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Section: Resultsmentioning

confidence: 99%