Contrasting effects of membrane enrichment with polyunsaturated fatty acids on phospholipid composition and cholesterol efflux from cholesterol-loaded J774 mouse or primary human macrophages

Fournier, Natalie; Benoist, Jean‐François; Allaoui, Fatima; Nowak, Maxime; Dakroub, Hani; Védie, Benoît; Paul, Jean–Louis

doi:10.1016/j.bbalip.2019.158536

Cited by 5 publications

(3 citation statements)

References 77 publications

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“…Furthermore, cytochrome P450 (CYP450) pathway was defined as the crucial signaling pathway associated with cellular lipid metabolism. Previous reports have indicated that arachidonic acid (AA) metabolism mediated by the CYP450 pathway was involved in macrophage foam cell formation 51 . In this study, mouse CYP4A14 (human CYP4A11, mouse CYP4A14 homolog 52 ) was identified as a downstream target of DKK1 to regulate SMC foam cell formation.…”

Section: Discussionmentioning

confidence: 89%

“…Previous reports have indicated that arachidonic acid (AA) metabolism mediated by the CYP450 pathway was involved in macrophage foam cell formation. 51 In this study, mouse CYP4A14 (human CYP4A11, mouse CYP4A14 homolog 52 ) was identified as a downstream target of DKK1 to regulate SMC foam cell formation. And CYP4A11 was significantly regulated by DKK1 in a C/EBPδ-dependent manner in SMCs, while CYP4A14 was considerably increased thereby indicating the involvement of the CYP4A14 in atherosclerosis.…”

Section: Discussionmentioning

confidence: 97%

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Dickkopf‐1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1

Li,

Zheng,

Zhang

et al. 2023

The FASEB Journal

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Vascular smooth muscle cells (VSMCs) are considered to be a crucial source of foam cells in atherosclerosis due to their low expression level of cholesterol exporter ATP‐binding cassette transporter A1 (ABCA1) intrinsically. While the definite regulatory mechanisms are complicated and have not yet been fully elucidated, we previously reported that Dickkopf‐1 (DKK1) mediates endothelial cell (EC) dysfunction, thereby aggravating atherosclerosis. However, the role of smooth muscle cell (SMC) DKK1 in atherosclerosis and foam cell formation remains unknown. In this study, we established SMC‐specific DKK1‐knockout (DKK1SMKO) mice by crossbreeding DKK1flox/flox mice with TAGLN‐Cre mice. Then, DKK1SMKO mice were crossed with APOE−/− mice to generate DKK1SMKO/APOE−/− mice, which exhibited milder atherosclerotic burden and fewer SMC foam cells. In vitro loss‐ and gain‐of‐function studies of DKK1 in primary human aortic smooth muscle cells (HASMCs) have proven that DKK1 prevented oxidized lipid‐induced ABCA1 upregulation and cholesterol efflux and promoted SMC foam cell formation. Mechanistically, RNA‐sequencing (RNA‐seq) analysis of HASMCs as well as chromatin immunoprecipitation (ChIP) experiments showed that DKK1 mediates the binding of transcription factor CCAAT/enhancer‐binding protein delta (C/EBPδ) to the promoter of cytochrome P450 epoxygenase 4A11 (CYP4A11) to regulate its expression. In addition, CYP4A11 as well as its metabolite 20‐HETE‐promoted activation of transcription factor sterol regulatory element‐binding protein 2 (SREBP2) mediated the DKK1 regulation of ABCA1 in SMC. Furthermore, HET0016, the antagonist of CYP4A11, has also shown an alleviating effect on atherosclerosis. In conclusion, our results demonstrate that DKK1 promotes SMC foam cell formation during atherosclerosis via a reduction in CYP4A11‐20‐HETE/SREBP2‐mediated ABCA1 expression.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 97%

Dickkopf‐1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1

Li,

Zheng,

Zhang

et al. 2023

The FASEB Journal

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“…PUFAs express impressive anti-inflammatory properties. Monounsaturated oleic acid is able to potentiate effects of anthocyanins on pro- and anti-inflammatory cytokines mRNA expression and synthesis [ 35 ], whereas there is some controversy with regard to the effect of PUFAs on macrophage cholesterol metabolism depending on the types of studied macrophage cultures (human vs. murine) [ 66 ].…”

Section: The Role Of Micronutrients and Biologically Active Compoundsmentioning

confidence: 99%

The Role of Diet in Regulation of Macrophages Functioning

et al. 2022

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The great importance of diet for health and high life-expectancy is established. The impact of nutrients on immune system is a point of growing research interest. Recent studies have found pro- and anti-inflammatory properties of some diet patterns and nutrients that can be used from the bench to the bedside for chronic low-grade inflammatory status correction. In this regard, the assessment of potential effects of nutrition on macrophage differentiation, proliferation, and functioning in health and disease is highly demanded. In this review, we present current data on the effects of nutrients on the macrophage functioning.

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Eicosapentaenoic acid membrane incorporation stimulates ABCA1-mediated cholesterol efflux from human THP-1 macrophages

Dakroub

Nowak

Benoist

et al. 2021

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Contrasting effects of membrane enrichment with polyunsaturated fatty acids on phospholipid composition and cholesterol efflux from cholesterol-loaded J774 mouse or primary human macrophages

Cited by 5 publications

References 77 publications

Dickkopf‐1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1

Dickkopf‐1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1

The Role of Diet in Regulation of Macrophages Functioning

Eicosapentaenoic acid membrane incorporation stimulates ABCA1-mediated cholesterol efflux from human THP-1 macrophages

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