Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Ying, Wei; Cheruku, Patali S.; Bazer, Fuller W.; Safe, Stephen; Zhou, Beiyan

doi:10.3791/50323

Cited by 238 publications

(214 citation statements)

References 21 publications

Supporting

Mentioning

205

Contrasting

Unclassified

Order By: Relevance

“…1 A and B). Monocytes from the bone marrow of healthy mice were isolated, cultivated in vitro, and polarized into M1-or M2-like macrophages using chemokine mixtures (25). We found that the two macrophage subtypes phagocytosed particles to a similar degree (Fig.…”

Section: Methodsmentioning

confidence: 74%

In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis

Kirschbaum

Sonner

Zeller

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Innate immune cells play a key role in the pathogenesis of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). Current clinical imaging is restricted to visualizing secondary effects of inflammation, such as gliosis and blood-brain barrier disruption. Advanced molecular imaging, such as iron oxide nanoparticle imaging, can allow direct imaging of cellular and molecular activity, but the exact cell types that phagocytose nanoparticles in vivo and how phagocytic activity relates to disease severity is not well understood. In this study we used MRI to map inflammatory infiltrates using high-field MRI and fluorescently labeled cross-linked iron oxide nanoparticles for cell tracking. We confirmed nanoparticle uptake and MR detectability ex vivo. Using in vivo MRI, we identified extensive nanoparticle signal in the cerebellar white matter and circumscribed cortical gray matter lesions that developed during the disease course (4.6-fold increase of nanoparticle accumulation in EAE compared with healthy controls, P < 0.001). Nanoparticles showed good cellular specificity for innate immune cells in vivo, labeling activated microglia, infiltrating macrophages, and neutrophils, whereas there was only sparse uptake by adaptive immune cells. Importantly, nanoparticle signal correlated better with clinical disease than conventional gadolinium (Gd) imaging (r, 0.83 for nanoparticles vs. 0.71 for Gd-imaging, P < 0.001). We validated our approach using the Food and Drug Administration-approved iron oxide nanoparticle ferumoxytol. Our results show that noninvasive molecular imaging of innate immune responses can serve as an imaging biomarker of disease activity in autoimmune-mediated neuroinflammation with potential clinical applications in a wide range of inflammatory diseases.MRI | nanoparticle imaging | USPIO | multiple sclerosis | EAE

show abstract

Section: Methodsmentioning

confidence: 74%

In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis

Kirschbaum

Sonner

Zeller

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Bone marrow macrophages were isolated from the femur and tibia of 14-day-old C57Bl/6 mice, cultured them in 15-cm dishes in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine, 10 mM Hepes (all Gibco, Invitrogen) and 15% L-929 cell-conditioned medium (LCM) containing M-CSF for 8 days, then induced their differentiation toward an intestinal M2 phenotype by treating for 24 h with IL-4 (20 ng/ml, Peprotech) and IL-10 (10 ng/ml, R&D Systems). Flow cytometry was then performed to assure the intestinal (M2) phenotype using markers for CD206 and CD163 [13]. Cocultured enteroids with niche components performed on three separate experiments with at least ten different enteroids evaluated per experimental group.…”

Section: Methodsmentioning

confidence: 99%

Intestinal Stem Cell Growth and Differentiation on a Tubular Scaffold with Evaluation in Small and Large Animals

et al. 2015

View full text Add to dashboard Cite

Aims To investigate the growth and differentiation of intestinal stem cells on a novel tubular scaffold in vitro and in vivo. Methods Intestinal progenitor cells from mice or humans were cultured with myofibroblasts, macrophages and/or bacteria, and evaluated in mice via omental implantation. Mucosal regeneration was evaluated in dogs after rectal mucosectomy followed by scaffold implantation. Results Intestinal progenitor cells differentiated into crypt-villi structures on the scaffold. Differentiation and scaffold coverage was enhanced by coculture with myofibroblasts, macrophages and probiotic bacteria, while the implanted scaffolds enhanced mucosal regeneration in the dog rectum. Conclusion Intestinal stem cell growth and differentiation on a novel tubular scaffold is enhanced through addition of cellular and microbial components, as validated in mice and dogs.

show abstract

“…With respect to macrophages, we cocultured WT ATB2s or Ltb4r1KO ATB2s with WT BM-derived macrophages (BMDMs) that had been stimulated with either LPS or IL-4/IL-13 to produce an M1-like or M2-like polarization state, respectively (42,43). WT ATB2s had a greater effect in stimulating CD69 expression in M1-like BMDMs compared with Ltb4r1KO ATB2s (Figure 5D) accompanied by greater production of the proinflammatory cytokines Tnfa, Il6, and Il1b ( Figure 5E).…”

Section: Cd5mentioning

confidence: 99%

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling

Ying

Wollam

Ofrecio

et al. 2017

Journal of Clinical Investigation

Self Cite

106

View full text Add to dashboard Cite

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Cited by 238 publications

References 21 publications

In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis

In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis

Intestinal Stem Cell Growth and Differentiation on a Tubular Scaffold with Evaluation in Small and Large Animals

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling

Contact Info

Product

Resources

About