Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts

Tulay, Pınar; Naja, Roy; Cascales-Roman, O.; Doshi, Alpesh; Serhal, Paul; SenGupta, Sioban

doi:10.1007/s10815-015-0585-0

Cited by 23 publications

(18 citation statements)

References 59 publications

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“…All other statistical analyses were performed using GraphPad Prism v6 software [ 26 ]. qRT-PCR and IHC HD5 counts were examined by applying an unpaired two-tailed Student’s t -test with the Welch correction, respectively.…”

Section: Methodsmentioning

confidence: 99%

Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease

et al. 2017

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Inability to distinguish Crohn's colitis from ulcerative colitis leads to the diagnosis of indeterminate colitis. This greatly effects medical and surgical care of the patient because treatments for the two diseases vary. Approximately 30 percent of inflammatory bowel disease patients cannot be accurately diagnosed, increasing their risk of inappropriate treatment. We sought to determine whether transcriptomic patterns could be used to develop diagnostic biomarker(s) to delineate inflammatory bowel disease more accurately. Four patients groups were assessed via whole-transcriptome microarray, qPCR, Western blot, and immunohistochemistry for differential expression of Human α-Defensin-5. In addition, immunohistochemistry for Paneth cells and Lysozyme, a Paneth cell marker, was also performed. Aberrant expression of Human α-Defensin-5 levels using transcript, Western blot, and immunohistochemistry staining levels was significantly upregulated in Crohn's colitis, p< 0.0001. Among patients with indeterminate colitis, Human α-Defensin-5 is a reliable differentiator with a positive predictive value of 96 percent. We also observed abundant ectopic crypt Paneth cells in all colectomy tissue samples of Crohn's colitis patients. In a retrospective study, we show that Human α-Defensin-5 could be used in indeterminate colitis patients to determine if they have either ulcerative colitis (low levels of Human α-Defensin-5) or Crohn's colitis (high levels of Human α-Defensin-5). Twenty of 67 patients (30 percent) who underwent restorative proctocolectomy for definitive ulcerative colitis were clinically changed to de novo Crohn's disease. These patients were profiled by Human α-Defensin-5 immunohistochemistry. All patients tested strongly positive. In addition, we observed by both hematoxylin and eosin and Lysozyme staining, a large number of ectopic Paneth cells in the colonic crypt of Crohn's colitis patient samples. Our experiments are the first to show that Human α-Defensin-5 is a potential candidate biomarker to molecularly differentiate Crohn's colitis from ulcerative colitis, to our knowledge. These data give us both a potential diagnostic marker in Human α-Defensin-5 and insight to develop future mechanistic studies to better understand crypt biology in Crohn's colitis.

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Section: Methodsmentioning

confidence: 99%

Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease

et al. 2017

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“…Os microRNAs são expressos nos mais diversos organismos eucariotos, inclusive em humanos. Foi verificado que há a expressão de miRNAs tanto em células germinativas quanto em embriões humanos 23 . Desde o final da década de 1970, quando ocorreu o nascimento do primeiro "bebê de proveta", já é discutida a utilização de embriões remanescentes de técnicas de fertilização in vitro, e até então, esta prática é vetada.…”

Section: Discussionunclassified

Mãe, obrigada pelos microRNAs

Junqueira¹,

Mayer²,

Poeta³

2018

Clin. Biomed. Res.

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RESUMOOs microRNAs (miRNAs) são pequenas moléculas de RNA não codificante que têm grande importância nos mais diversos processos celulares, pois atuam na regulação da expressão gênica pós-transcricional. Estima-se que estes RNAs tenham controle de, em média, 30% da regulação de genes codificantes de proteínas em mamíferos. Da mesma forma, na fase zigótica do desenvolvimento embrionário, os miRNAs maternos desempenham funções notáveis e são fundamentais para a degradação dos próprios transcritos maternos. Este evento é determinante para a transição materno-zigótica, momento onde o zigoto passa a expressar completamente e de maneira independente seus próprios mRNAs, e; portanto, são vitais para o desenvolvimento inicial do embrião. O presente estudo, através de uma revisão narrativa de literatura, busca descrever os mecanismos de ação de miRNAs maternos presentes em zigotos de diversas espécies durante o desenvolvimento embrionário. Foram selecionados estudos disponíveis na base de dados PubMed através da busca utilizando palavras-chave descritas pelos Descritores em Ciências da Saúde (DeCS). ABSTRACTMicroRNAs (miRNAs) are small molecules of non-coding RNA that have great importance in the most diverse cellular processes, since they act in the post-transcriptional regulation of gene expression. It is estimated that these RNAs have a control of, on average, 30% of the regulation of protein-encoding genes in mammals. Likewise, in the zygotic phase of embryonic development, maternal miRNAs perform remarkable functions and are fundamental for the degradation of the maternal transcripts themselves. This event is determinant for the maternal-to-zygotic transition, at which moment the zygote begins to express completely and independently its own miRNAs, and is therefore vital for the initial development of the embryo. The present study, through a review of the literature, aims to describe the mechanisms of action of maternal miRNAs present in zygotes of different species during embryonic development. We selected only the studies listed in the PubMed database through the search using keywords described by the Health Sciences Descriptors (DeCS).

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“…By contrast, most zygotic miRNAs are derived from maternal oocytes (Tang et al, ). Altered miRNA expression in cumulus cells could result in a poor ovarian response in women undergoing in vitro fertilization (Karakaya et al, ), and some miRNAs are highly specific to the blastocyst and are not expressed in the oocytes (Tulay et al, ). In addition, sperm‐borne miR‐34c‐5p is thought to initiate the first cleavage divisions in the mouse (Liu et al, ), and is positively correlated with intracytoplasmic sperm injection outcomes, as well as high‐quality embryos, implantation, pregnancy and live birth in humans (Cui et al, ), and the presence of miR‐34c‐5p indicates viability and high‐quality spermatozoa in humans (Abu‐Halima et al, ).…”

Section: Mirnas As Regulators Of Zygotic and Early Embryo Reprogrammingmentioning

confidence: 99%

Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri‐implantation embryos

et al. 2018

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MicroRNAs (miRNAs) are active regulators of numerous biological and physiological processes including most of the events of mammalian reproduction. Understanding the biological functions of miRNAs in the context of mammalian reproduction will allow a better and comparative understanding of fertility and sterility in male and female mammals. Herein, we summarize recent progress in miRNA-mediated regulation of mammalian reproduction and highlight the significance of miRNAs in different aspects of mammalian reproduction including the biogenesis of germ cells, the functionality of reproductive organs, and the development of early embryos. Furthermore, we focus on the gene expression regulatory feedback loops involving hormones and miRNA expression to increase our understanding of germ cell commitment and the functioning of reproductive organs. Finally, we discuss the influence of miRNAs on male and female reproductive failure, and provide perspectives for future studies on this topic.

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Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts

Cited by 23 publications

References 59 publications

Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease

Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease

Mãe, obrigada pelos microRNAs

Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri‐implantation embryos

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