Investigation of multiple whole smoke dosimetry techniques using a VITROCELL®VC10® smoke exposure system

Abstract

Graphical abstract

Systems toxicology assessment of a representative e-liquid formulation using human primary bronchial epithelial cells

“…For example, a recent study used regulatory safety toxicological tests to assess whether addition of flavoring substances to an unflavored product contributes to the toxicity of the gas–vapor phase [7]. Such tests can now also be conducted by using more advanced whole-aerosol exposure systems [8,9].…”

Cinnamon-flavored electronic cigarette liquids and aerosols induce oxidative stress in human osteoblast-like MG-63 cells

“…On average, the puff lasts about 3.20 s with 13 s interval. In another study, Hua et al [51] report an average puff lasting 4.3 s with the range of duration being from 1.9 to 8.3 s. The wide variation among e-cigarette users makes the development of standard exposure systems difficult, including the use of VITROCELL smoke exposure system modified for e-cigarette aerosol exposure [[52], [53], [54]]. This current study generates aerosol condensate from 40 puffs of 3 s with 27 s interval, which falls within the range of puff duration observed [51] and the method used by previous aerosolized exposure studies [15,18,42,46,48].…”

Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module

“…Photometers were purchased from Vitrocell® Systems, GmBH. For combustible cigarettes, photometers were harmonized to whole smoke using the HCI regimen [19] and a diluting airflow of 1.5 L/min. For ENDS and eHTP consumables, photometers were harmonized to whole aerosol using a mHCI and mISO regimen, respectively.…”