Investigation of Nanoemulsion System for Transdermal Delivery of Domperidone: Ex-vivo and in vivo Studies

Akhter, Sohail; Jain, Gaurav; Ahmad, Feroz; Khar, Roop K.; Jain, Neelu; Khan, Zeenat; Talegaonkar, Sushama

doi:10.2174/157341308786306071

Cited by 62 publications

(35 citation statements)

References 30 publications

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“…Similar increasing of transdermal domperidone delivery was observed when decreasing Tween 80 concentration. 23) Microemulsion types (water-in-oil or oil-in-water) would affect the delivery efficacy of curcumin. 24) F127 was incorporated into microemulsions to stabilize the microemulsions.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Activity of Curcumin-Loaded Myristic Acid Microemulsions against <i>Staphylococcus epidermidis</i>

Liu

Huang

2012

Chem. Pharm. Bull.

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The bactericidal properties of myristic acid and curcumin were revealed in a number of studies. However, whether curcumin-loaded myristic acid microemulsions can be used to inhibit Staphylococcus epidermidis, which causes nosocomial infections, has not been reported. Our aim was to develop curcumin-loaded myristic acid microemulsions to inhibit S. epidermidis on the skin. The interfacial tension, size distribution, and viscosity data of the microemulsions were characterized to elucidate the physicochemical properties of the curcumin microemulsions. Curcumin distribution in neonate pig skin was visualized using confocal laser scanning microscopy. Dermal curcumin accumulation (326 µg/g skin) and transdermal curcumin penetration (87 µg/cm 2 /d) were obtained with the microemulsions developed herein. Curcumin at the concentration of 0.86 µg/mL in the myristic acid microemulsion could inhibit 50% of the bacterial growth, which was 12 times more effective than curcumin dissolved in dimethyl sulfoxide (DMSO). The cocktail combination of myristic acid and curcumin in the microemulsion carrier synergistically inhibited the growth of S. epidermidis. The results we obtained highlight the potential of using curcumin-loaded microemulsions as an alternative treatment for S. epidermidis-associated diseases and acne vulgaris.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Activity of Curcumin-Loaded Myristic Acid Microemulsions against <i>Staphylococcus epidermidis</i>

Liu

Huang

2012

Chem. Pharm. Bull.

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“…Similar increasing of domperidone delivery was observed in skin when decreasing Tween 20 concentration. 35) Microemulsion structure (water-in-oil or oil-in-water) also affects the delivery efficacy of drug. 14) Emulsions would change from water-in-oil to oil-in-water types when the water content increased.…”

Section: Inhibition Of P Acnes By Curcumin Vehiclesmentioning

confidence: 99%

In Vitro Anti-Propionibacterium Activity by Curcumin Containing Vesicle System

Liu

Huang

2013

Chem. Pharm. Bull.

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Propionibacterium acnes acts a critical role in the development of inflammatory acne when it overgrows in pilosebaceous units. The spread of multiple drug resistance bacteria indicates a growing need for new antimicrobial agents. The objective of this study is to develop lipid vehicles to deliver curcumin and inhibit P. acnes in the skin. The inhibitory activities of the curcumin containing vehicles against P. acnes were studied by the bioluminescence assay. Curcumin accumulation patterns in neonate pig skin were studied using Franz diffusion cells and confocal laser scanning microscopy. The physicochemical properties of the curcumin containing vehicles including interfacial tension, size distribution and viscosity were analyzed. Significant curcumin accumulation (362±8 µg/g skin) was observed with the lipid vehicles developed herein. Curcumin (0.43 µg/mL) in the vehicles significantly inhibited the growth of P. acnes. Confocal laser scanning microscopy confirmed the formation of curcumin reservoir in the skin by the curcumin-loaded vehicles. Curcumin-loaded vehicles could efficiently accumulate in the skin and inhibit P. acnes in vitro. Our results highlight the potential of using vehicles containing lauric acid and curcumin as an alternative treatment for acne vulgaris.

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“…[4][5][6][7] It has been reported that ME may enhance transdermal drug delivery by improving drug solubility, thereby increasing the molecular concentration gradient, as well as by altering the SC structure. 8,9 In the current study, an ME vehicle of total flavone of RA (TFRA) was formulated, with the aim of improving its transdermal delivery. The characteristics and skin permeation of the prepared MEs were evaluated in vitro and in vivo.…”

mentioning

confidence: 99%

Preparation and evaluation of microemulsion-based transdermal delivery of total flavone of rhizoma arisaematis

Shen

Zhang

Wang

et al. 2014

IJN

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The aims of the present study were to investigate the skin permeation and cellular uptake of a microemulsion (ME) containing total flavone of rhizoma arisaematis (TFRA), and to evaluate its effects on skin structure. Pseudo-ternary phase diagrams were constructed to evaluate ME regions with various surfactants and cosurfactants. Eight formulations of oil-in-water MEs were selected as vehicles, and in vitro skin-permeation experiments were performed to optimize the ME formulation and to evaluate its permeability, in comparison to that of an aqueous suspension. Laser scanning confocal microscopy and fluorescent-activated cell sorting were used to explore the cellular uptake of rhodamine 110-labeled ME in human epidermal keratinocytes (HaCaT) and human embryonic skin fibroblasts (CCC-ESF-1). The structure of stratum corneum treated with ME was observed using a scanning electron microscope. Furthermore, skin irritation was tested to evaluate the safety of ME. ME formulated with 4% ethyl oleate (weight/weight), 18% Cremophor EL ® (weight/weight), and 18% Transcutol ® P, with 1% Azone to enhance permeation, showed good skin permeability. ME-associated transdermal fluxes of schaftoside and isoschaftoside, two major effective constituents of TFRA, were 3.72-fold and 5.92-fold higher, respectively, than those achieved using aqueous suspensions. In contrast, in vitro studies revealed that uptake by HaCaT and CCC-ESF-1 cells was lower with ME than with an aqueous suspension. Stratum corneum loosening and shedding was observed in nude mouse skin treated with ME, although ME produced no observable skin irritation in rabbits. These findings indicated that ME enhanced transdermal TFRA delivery effectively and showed good biocompatibility with skin tissue.

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Investigation of Nanoemulsion System for Transdermal Delivery of Domperidone: Ex-vivo and in vivo Studies

Cited by 62 publications

References 30 publications

Antimicrobial Activity of Curcumin-Loaded Myristic Acid Microemulsions against <i>Staphylococcus epidermidis</i>

Antimicrobial Activity of Curcumin-Loaded Myristic Acid Microemulsions against <i>Staphylococcus epidermidis</i>

In Vitro Anti-Propionibacterium Activity by Curcumin Containing Vesicle System

Preparation and evaluation of microemulsion-based transdermal delivery of total flavone of rhizoma arisaematis

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