Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth

Papagerakis, Pétros; Berdal, Ariane; Mesbah, Mohand; Peuchmaur, M.; Malaval, Luc; Nydegger, Jason; Simmer, James P.; MacDougall, Mary

doi:10.1016/s8756-3282(01)00683-4

Cited by 181 publications

(149 citation statements)

References 59 publications

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“…32 Natively, amelogenin is expressed by ameloblasts and was selected to simulate odontogenesis and/or cementogenesis, its effect to promote differentiation of odotoblasts and cementoblasts. 30,39,40 Consistently, our pilot study showed that amelogenin promote DPSC's differentiation into odontoblast-like cells (data not shown). PLGA mS-encapsulating these bioactive cues were prepared using double-emulsion technique as per our previous work.…”

Section: Methodssupporting

confidence: 68%

Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

Lee

Hajibandeh

Suzuki

et al. 2014

Tissue Engineering Part A

178

168

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Section: Methodssupporting

confidence: 68%

Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

Lee

Hajibandeh

Suzuki

et al. 2014

Tissue Engineering Part A

178

168

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“…[28][29][30] They are secreted by certain tumor cells with metastatic affinity to bone tissue, as for example by breast, prostate, lung and other cancer cells. 2,3,12,13,18,[31][32][33][34] In addition, they show various functions; OPN can bind to a number of receptors such as integrins as well as to certain variant forms of CD44, [35][36][37] it can act as a cytokine 29 and has function in the urinary tract.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of osteopontin and bone sialoprotein II is related to reduced colony formation and metastasis formation of MDA-MB-231 human breast cancer cells

2003

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Osteopontin (OPN), bone sialoprotein (BSPII), and osteonectin (ON) belong to a family of glycoproteins, which have been linked to cancer metastasis and progression. Here, we report on the selection of antisense oligonucleotides (ASOs), which are effective in reducing their protein levels. In human MDA-MB-231 breast cancer cells, the maximum inhibition of protein expression ranged from 84% (OPN) to 75% (BSPII) and 70% (ON). Erucylphospho-NNN-trimethylpropanolamine (ErPC 3 ) was used as positive control and combination partner. Exposure to ErPC 3 inhibited colony formation of MDA-MB-231 cells by 11% (10 mM), 45% (14 mM) and 78% (20 mM). The clonogenicity of breast cancer cells was reduced by 15%, 11%, 8% (5 mM), 39%, 19%, 14% (10 mM) and 46%, 39%, 21% (20 mM) in response to ASO-OPN-04, ASO-BSPII-06 and ASO-ON-03, respectively. Combination of ErPC 3 with the ASOs caused additive combination effects. Pre-exposure to the ASOs, but not to the NSO, inhibited formation of osteolytic metastasis in three of four (ASO-OPN-04, Po0.03) and two of four (ASO-BSPII-06) nude rats, and reduced metastasis lesions significantly (T/C% ¼ 4.3 and 9.1, P ¼ 0.05, respectively). We conclude that downregulation of OPN and BSPII reduces colony formation of MDA-MB-231 cells and formation of osteolytic metastasis in nude rats.

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“…RNA samples that had not been reversetranscribed were included to check for the absence of DNA contamination. Primers sequences for PTH/PTHrpreceptor (PTH/PTHrpR) (Stewart et al, 1999), osteonectin (ON) (Gronthos et al, 1999), dentin sialophosphoprotein (DSPP) (Papagerakis et al, 2002) and glyceraldehyde phosphate dehydrogenase (GAPDH) (Bluteau et al, 1999) and annealing temperatures are detailed in table 1. Amplifications were carried out in an Eppendorf master cycler (VWR, Brumath, France) under the following conditions: denaturation for 3 minutes at …”

Section: Rt-pcrmentioning

confidence: 99%

Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

Lopez-Cazaux¹,

Bluteau²,

Magne³

et al. 2006

eCM

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In vitro approaches have extensively been developed to study reparative dentinogenesis. While dental pulp is a source of unidentified progenitors able to differentiate into odontoblast-like cells, we investigated the effect of two media; MEM (1.8mM Ca and 1mM Pi) and RPMI 1640 (0.8mM Ca and 5mM Pi) on the behaviour of human dental pulp cells. Our data indicate that MEM significantly increased cell proliferation and markedly enhanced the proportion of α-smooth muscle actin positive cells, which represent a putative source of progenitors able to give rise to odontoblast-like cells. In addition, MEM strongly stimulated alkaline phosphatase activity and was found to induce expression of transcripts encoding dentin sialophosphoprotein, an odontoblastic marker, without affecting that of parathyroid hormone/parathyroid hormone related protein-receptor and osteonectin. In conclusion, these observations demonstrate that not only proliferation but also differentiation into odontoblast-like cells was induced by rich calcium and poor phosphate medium (MEM) as compared to RPMI 1640. This study provides important data for the determination of the optimal culture conditions allowing odontoblast-like differentiation in human pulp cell culture.

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Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth

Cited by 181 publications

References 59 publications

Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

Downregulation of osteopontin and bone sialoprotein II is related to reduced colony formation and metastasis formation of MDA-MB-231 human breast cancer cells

Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

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