Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

Lee, Chang H.; Hajibandeh, Jeffrey; Suzuki, Tsuyoshi; Fan, Andrew; Shang, Peng; Mao, Jeremy J.

doi:10.1089/ten.tea.2013.0386

Cited by 179 publications

(194 citation statements)

References 57 publications

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“…[44][45][46][47][48] The discrepancy in size between scaffolds tested in vitro and scaffolds applied in bone defects are often large, so we sought to assess the osteogenic potential on large, clinically relevant scaffolds. Parallels between static in vitro culturing and an in vivo environment in terms of nutrient flow and micro environmental conditions are limited.…”

Section: Discussionmentioning

confidence: 99%

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Jensen

Kraft

Lysdahl

et al. 2015

Tissue Engineering Part A

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In this study, we sought to assess the osteogenic potential of human dental pulp stem cells (DPSCs) on three different polycaprolactone (PCL) scaffolds. The backbone structure of the scaffolds was manufactured by fused deposition modeling (PCL scaffold). The composition and morphology was functionalized in two of the scaffolds. The first underwent thermal induced phase separation of PCL infused into the pores of the PCL scaffold. This procedure resulted in a highly variable micro- and nanostructured porous (NSP), interconnected, and isotropic tubular morphology (NSP-PCL scaffold). The second scaffold type was functionalized by dip-coating the PCL scaffold with a mixture of hyaluronic acid and β-TCP (HT-PCL scaffold). The scaffolds were cylindrical and measured 5 mm in height and 10 mm in diameter. They were seeded with 1×10(6) human DPSCs, a cell type known to express bone-related markers, differentiate into osteoblasts-like cells, and to produce a mineralized bone-like extracellular matrix. DPSCs were phenotypically characterized by flow cytometry for CD90(+), CD73(+), CD105(+), and CD14(-). DNA, ALP, and Ca(2+) assays and real-time quantitative polymerase chain reaction for genes involved in osteogenic differentiation were analyzed on day 1, 7, 14, and 21. Cell viability and distribution were assessed on day 1, 7, 14, and 21 by fluorescent-, scanning electron-, and confocal microscopy. The results revealed that the DPSCs expressed relevant gene expression consistent with osteogenic differentiation. The NSP-PCL and HT-PCL scaffolds promoted osteogenic differentiation and Ca(2+) deposition after 21 days of cultivation. Different gene expressions associated with mature osteoblasts were upregulated in these two scaffold types, suggesting that the methods in which the scaffolds promote osteogenic differentiation, depends on functionalization approaches. However, only the HT-PCL scaffold was also able to support cell proliferation and cell migration resulting in even cell dispersion throughout the scaffold. In conclusion, DPSCs could be a possible alternate cell source for bone tissue engineering. The HT-PCL scaffold showed promising results in terms of promoting cell migration and osteogenic differentiation, which warrants future in vivo studies.

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Section: Discussionmentioning

confidence: 99%

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Jensen

Kraft

Lysdahl

et al. 2015

Tissue Engineering Part A

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“…9 With this aim, various strategic scaffold multiphasic architectures have been developed: bone scaffolds combined with an occlusive membrane, 10,11 layered biphasic scaffolds for bone and ligament compartments 12 -16 and layered triphasic scaffolds for cementum, periodontal ligament and bone compartment. 17 Regenerative approaches toward in situ periodontal tissue regeneration are frequently based on endogenous resources such as cells and growth factors. 4 The most exploited strategy involves a scaffolding material (e.g.…”

Section: Introductionmentioning

confidence: 99%

Integrated three‐dimensional fiber/hydrogel biphasic scaffolds for periodontal bone tissue engineering

Puppi

Migone

Grassi

et al. 2016

Polymer International

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Combining a tissue engineering scaffold made of a load-bearing polymer with a hydrogel represents a powerful approach to enhancing the functionalities of the resulting biphasic construct, such as its mechanical properties or ability to support cellular colonization. This research activity was aimed at the development of biphasic scaffolds through the combination of an additively manufactured poly( -caprolactone) (PCL) fiber construct and a chitosan/poly( -glutamic acid) polyelectrolyte complex hydrogel. By investigating a set of layered structures made of PCL or PCL/hydroxyapatite composite, biphasic scaffold prototypes with good integration of the two phases at the macroscale and microscale were developed. The biphasic constructs were able to absorb cell culture medium up to 10-fold of their weight, and the combination of the two phases had a significant influence on compressive mechanical properties compared with hydrogel or PCL scaffold alone. In addition, due to the presence of chitosan in the hydrogel phase, biphasic scaffolds exerted a broad-spectrum antibacterial activity. The developed biphasic systems appear well suited for application in periodontal bone regenerative approaches in which a biodegradable porous structure providing mechanical stability and a hydrogel phase functioning as absorbing depot of endogenous proteins are simultaneously required.

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“…[23] However, it remains difficult to create three-dimensional scaffolds using these techniques without the presence of a more complex and customizable system such as a 3-D printer that could position fibers in directions relevant to the anatomical structure of tissue we attempt to model, thereby recapitulating its unique geometric complexity. Additive manufacturing has been used extensively for guided tissue regeneration of PDL-like structures, [10, 12] but micro-level control over scaffold structure is constrained by limited resolution and material selection. To address these limitations, our approach focuses on using a combination of 3-D printing and micropatterning to create a scaffold with the dual function of presenting features on the macro and micro-level for guidance of bulk and oriented tissue formation (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…[9, 10] Costa et al and Vaquette et al employed PCL for the bone compartment and electrospun PCL for the PDL region, [11] while Lee et al used a layered 3-D printed scaffold with three PCL interphases for the cementum, PDL, and alveolar bone. [12] However, the precise effect of topography on the guidance of PDL-like tissue formation in vivo has not been thoroughly explored to understand how micro-scale patterning affects the regenerative outcome.…”

Section: Introductionmentioning

confidence: 99%

Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In Vivo

Pilipchuk

Monje

Jiao

et al. 2016

Adv Healthcare Materials

105

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Scaffold design incorporating multi-scale cues for clinically-relevant, aligned tissue regeneration has potential to improve structural and functional integrity of multi-tissue interfaces. The objective of this pre-clinical study was to develop poly(ε-caprolactone) (PCL) scaffolds with mesoscale and microscale architectural cues specific to human ligament progenitor cells and assess their ability to form aligned bone-ligament-cementum complexes in vivo. PCL scaffolds were designed to integrate a 3D printed bone region with a micropatterned PCL thin film consisting of grooved pillars. The patterned film region was seeded with human ligament cells, fibroblasts transduced with BMP-7 genes seeded within the bone region, and a tooth dentin segment positioned on the ligament region prior to subcutaneous implantation into a murine model. Results indicated increased tissue alignment in vivo using micropatterned PCL films, compared to random-porous PCL. At 6 weeks, 30um groove depth significantly enhanced oriented collagen fiber thickness, overall cell alignment, and nuclear elongation relative to 10um groove depth. This study demonstrates for the first time that scaffolds with combined hierarchical mesoscale and microscale features can align cells in vivo for oral tissue repair with potential for improving the regenerative response of other bone-ligament complexes.

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Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

Cited by 179 publications

References 57 publications

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Integrated three‐dimensional fiber/hydrogel biphasic scaffolds for periodontal bone tissue engineering

Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In Vivo

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