Investigation of osteogenic responses of Fe-incorporated micro/nano-hierarchical structures on titanium surfaces

Abstract

Fe incorporated micro/nano topographical titanium substrates are fabricated to synergistically regulate osteogenic responses in vitro and osseointegration in vivo.

Zn‐incorporation with graphene oxide on Ti substrates surface to improve osteogenic activity and inhibit bacterial adhesion

“…Besides, GelMA‐PBA membrane was immobilized onto the GO‐Zn samples with free radical polymerization. The surface wettability could direct or indirect affect the biological responses of biomaterials (Shen et al, ; Yuan et al, ). In this work, the water contact angle of GO‐Zn/GeMA‐PBA was 56.0 ± 7.6° (Figure a), which was consistent with a previous study (Liu et al, ).…”

“…Protein adsorption is the first biological event after implantation of biomaterials, which is prerequisite for cell adhesion and proliferation (Yuan et al, ). The significant difference of BSA adsorption between bare Ti group and modified groups was caused by the two‐dimensional GO, which providing much more binding sites for protein adsorption (Figure a).…”

“…To evaluate the osteoblasts proliferation, MTT assay was carried out (Tao et al, ; Yuan et al, ). Osteoblasts suspension (2 × 10 4 cells/cm 2 ) were grown on each sample for 1, 4, and 7 days.…”

“…Osteoblasts (1 × 104 cells/cm 2 ) were cultured on various Ti substrates. Alizarin Red S staining was performed after culture for 14 days (Yuan et al, ). Briefly, the samples with osteoblasts were fixed with paraformaldehyde (4%, v/v) and each well was stained with 200 μL of Alizarin Red S (40 m M pH 4.1).…”

Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

“…A number of studies have proved that strategies, such as engineering surface topography (micro/nanostructures), [19][20][21] calcium phosphate coatings, 7,22,23 metallic elements, 24,25 extracellular matrix (ECM) proteins, 26,27 biomolecular coatings incorporating growth factors, [28][29][30] and so forth, can distinctly promote osseointegration by positively regulate cell adhesion, proliferation, and differentiation. Independently, growth factors play important roles in tissue repair and regeneration.…”