Investigation of Possible Genotoxic and Cytotoxic Effects of Differential Boron Compounds  in CCL 62 (Hela Contaminant) Human Amniotic Ephitelial Cell Line

Kahraman, Erkan; Gürhan, İsmet Deliloğlu; Korkmaz, Mehmet

doi:10.5455/medscience.2012.01.8046

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…Various concentrations (0, 1.25, 2.5, 5, 10, 20, 40, 80, 160, 320, 640 and 1,280 mg/l) of LMD were tested in blood cultures. The concentrations were selected according to previous studies Arslan et al 2008;Kahraman et al 2013). MN and CA rates were assessed in peripheral lymphocytes and the method that was used for the preparation of the peripheral lymphocytes is explained in sections below.…”

Section: Experimental Designmentioning

confidence: 99%

Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

et al. 2015

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Boron compounds have an ability of supporting antioxidant properties in human and animal tissues. Lithium metaborate dihydrate (LiBO 2-2H 2 O; LMD) is commonly used in nonlinear optic materials, cellular phones and pagers. But, there are limited data on the genotoxic and antioxidant effects of LMD in cultured human whole blood cells. The aim of this study was to evaluate for the genotoxicity and antioxidant/oxidant activity of LMD on human whole blood lymphocytes (n = 5). LMD was applied at various concentrations (0-1,280 lg/ml) to cultured blood samples. Antioxidant/oxidant activity was evaluated by measuring the total oxidant status (TOS) and total antioxidant capacity levels. Micronuclei and chromosomal aberration tests were used in genotoxicity studies. Our results clearly revealed that all tested concentrations of LMD were found to be non-genotoxic when compared to that of the control group. In addition, LMD exhibited antioxidant activities at low concentrations. In addition the TOS levels were not changed at all concentrations of LMD. Consequently, our results clearly demonstrated that LMD is non-genotoxic and it has an important antioxidant potential in vitro.

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Section: Experimental Designmentioning

confidence: 99%

Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

et al. 2015

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“…The growth kinetic was determined with batch culture mode in a 6-well plate (Greiner, Germany) according to the previously described protocols (Freshney 2010;Kahraman et al 2013). Briefly, HeLa cells were daily trypsinized from one well and the viability was determined with dye exclusion method.…”

Section: Growth Kinetics and Cell Adhesion Tests Of Hela Cellsmentioning

confidence: 99%

The use of Toxoplasma gondii tachyzoites produced in HeLa cells adhered to Cytodex 1 microcarriers as antigen in serological assays: an application of microcarrier technology

et al. 2019

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Toxoplasma gondii can infect nearly all warm-blooded animals, including humans. In the laboratory diagnosis of toxoplasmosis, serological tests have importance in detecting antibody response. Traditionally T. gondii tachyzoites grown in vivo are being used as an antigen source in serological assays. Currently, tachyzoites produced in vitro are being tested as an antigen source in order to decrease animal use. Microcarrier technology allowed us to grow anchorage-dependent host cells on microcarrier suspension in short time and approximately 10 times more than traditional flask technique. The ability of T. gondii tachyzoites to grow in host cells adhered to microcarriers has not been analyzed yet. In this study, we aimed to develop a novel in vitro culture method to produce T. gondii tachyzoites abundantly using HeLa cells adhered to Cytodex 1 microcarriers. Initially, the growth of HeLa cells adhered to Cytodex 1 was analyzed using RPMI 1640, DMEM, and EMEM. Next, HeLa cells with a concentration of 1 9 10 5cells/ml and 2 9 10 5 cells/ml were adhered to Cytodex 1 and grown in spinner flasks. Then, T. gondii tachyzoites were inoculated with 1:1 and 2:1 cell:tachyzoite ratios to HeLa cells adhered to microcarriers in spinner flaks. During continuous production in spinner flasks, tachyzoites were harvested at the 2nd, 4th, and 7th day of culture and the quality of antigens produced from these tachyzoites were tested in ELISA and Western Blotting using sera of patients with toxoplasmosis. The optimization studies showed that finest HeLa inoculation value was 2 9 10 5 cells/ml using RPMI 1640, and the cell:tachyzoite ratio to obtain the highest tachyzoite yield (17.1 9 10 7) was 1:1 at the 4th day of inoculation. According to the results of ELISA comparing HeLa cell and mouse derived antigens, the highest correlation with mouse antigen was achieved at the 4th day of HeLa cell culture with 1:1 HeLa:tachyzoite ratio (P \ 0.0001). The sensitivity and specificity ratios of ELISA were 100%. In addition, Western blotting banding patterns Saime İsmet Deliloglu Gürhan, Mert Döşkaya were the advisors of the BS student Pelin Saglam Metiner.

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“…Besides, BA, BP and DPD affected proliferation of CCL 62 cell lines in a time, compound‐ and dose‐dependent manner, nevertheless no evidence was observed suggesting these compounds lead to an enhance or decrease in the level of existing cytogenetic defects in these cell lines (Kahraman et al . ). According to the study of Kutlu et al ., although there is a potential of boric acid to occur various complexes by interacting physically, chemically and biologically inside the ecosystem, water of Seydisuyu stream has not led to mutagenicity such as frameshift/base‐pair substitution in Salmonella typhimurium strains (Kutlu et al .…”

Section: Introductionmentioning

confidence: 97%

Toxicity and mutagenicity assessment of a boron mine drainage water

Korkmaz

Sönmez

Çelik

et al. 2017

Water & Environment J

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This study was aimed to assess toxicity of boron mine drainage (BMD) water in Kırka borough of Turkey, which is one of the most important boron deposit sites in the world. Three complementary tests were done to assess the toxicity of BMD water. Root growth inhibition tests showed that EC50 value of BMD water sample was approximately 3.85% of concentration. Furthermore, mutagenicity was analysed utilising Ames Salmonella Mutagenicity Test and Muta‐ChromoPlate kit system (Environmental Biodetection Products Inc., Ontario, Canada). Salmonella typhimurium TA 98 strain for all concentrations and Salmonella typhimurium TA100 strain for the highest concentrations of BMD water in the absence of metabolic activation significantly stimulated mutagenic activity and increased the numbers of revertant colonies for several concentrations compared to spontaneous revertant colonies. RAPD‐PCR result and the polymorphism percentage proved that the effect of boron contamination was seen on DNA level.

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Investigation of Possible Genotoxic and Cytotoxic Effects of Differential Boron Compounds in CCL 62 (Hela Contaminant) Human Amniotic Ephitelial Cell Line

Abstract: Özet Epidemiyolojik ve in vitro çalışmalarında

Cited by 8 publications

References 13 publications

Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

The use of Toxoplasma gondii tachyzoites produced in HeLa cells adhered to Cytodex 1 microcarriers as antigen in serological assays: an application of microcarrier technology

Toxicity and mutagenicity assessment of a boron mine drainage water

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