Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide

Ebrahimi, Maryam; Ai, Jafar; Biazar, Esmaeil; Faridi‐Majidi, Reza; Hajati, Jamshid; Ebrahimi‐Barough, Somayeh; K, Saeed Heidari

doi:10.1080/10667857.2017.1317065

Cited by 10 publications

(7 citation statements)

References 47 publications

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“…The 2D surfaces provide a high surface area to mass ratio with remarkable mechanical properties [ 101 ]. The advantage of this fabrication method is to create highly organized patterns of fibers that mechanically guide seeded cells to regenerate the damage [ 104 ]. The mechanical properties of silk mats are a function of the solvent system, the fiber arrangement, and the mechanical properties of the individual fibers themselves.…”

Section: Fabrication Methods and Platforms For Regenerated Sf-basementioning

confidence: 99%

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

et al. 2021

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Silk refers to a family of natural fibers spun by several species of invertebrates such as spiders and silkworms. In particular, silkworm silk, the silk spun by Bombyx mori larvae, has been primarily used in the textile industry and in clinical settings as a main component of sutures for tissue repairing and wound ligation. The biocompatibility, remarkable mechanical performance, controllable degradation, and the possibility of producing silk-based materials in several formats, have laid the basic principles that have triggered and extended the use of this material in regenerative medicine. The field of neural soft tissue engineering is not an exception, as it has taken advantage of the properties of silk to promote neuronal growth and nerve guidance. In addition, silk has notable intrinsic properties and the by-products derived from its degradation show anti-inflammatory and antioxidant properties. Finally, this material can be employed for the controlled release of factors and drugs, as well as for the encapsulation and implantation of exogenous stem and progenitor cells with therapeutic capacity. In this article, we review the state of the art on manufacturing methodologies and properties of fiber-based and non-fiber-based formats, as well as the application of silk-based biomaterials to neuroprotect and regenerate the damaged nervous system. We review previous studies that strategically have used silk to enhance therapeutics dealing with highly prevalent central and peripheral disorders such as stroke, Alzheimer’s disease, Parkinson’s disease, and peripheral trauma. Finally, we discuss previous research focused on the modification of this biomaterial, through biofunctionalization techniques and/or the creation of novel composite formulations, that aim to transform silk, beyond its natural performance, into more efficient silk-based-polymers towards the clinical arena of neuroprotection and regeneration in nervous system diseases.

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Section: Fabrication Methods and Platforms For Regenerated Sf-basementioning

confidence: 99%

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

et al. 2021

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“…In previous work, we have successfully designed nanofibrous silk mat [45]. Briefly, raw B. mori silk fibres were boiled for half an hour in a 0.5% Na 2 CO 3 , and rinsed thoroughly with water to remove the glue-like sericin proteins surrounding the fibroin filaments and dried in air.…”

Section: Production Of Nanofibrous Matsmentioning

confidence: 99%

“…The electro-spun mat was rolled around the cylindrical rod to form a 3D tubular structure (10 mm in length with the inner diameter 2 mm), sealed and maintained in this form using a TFA solvent (Figure 1(B)). Schwann cells were obtained from sciatic nerves of 8-day-old rats according to the method described elsewhere [45]. For this experiment 30 male Wistar white rats aged approximately 4-8 weeks at the beginning of the experiment and weighing 180-220 g were used.…”

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

In vivo assessment of a nanofibrous silk tube as nerve guide for sciatic nerve regeneration

Ebrahimi

Biazar

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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A nanofibrous silk nerve conduit has been evaluated for its efficiency based on the promotion of peripheral nerve regeneration in rats. The designed tubes with or without Schwann cells were implanted into a 10 mm gap in the sciatic nerves of the rats. Four months after the surgery, the regenerated nerves were monitored and evaluated by macroscopic assessments and histology. The results demonstrated that the nanofibrous grafts, especially in the presence of Schwann cells, enabled reconstruction of the rat sciatic nerve trunk with a restoration of nerve continuity and formation of nerve fibres with myelination. Histological data demonstrated the presence of Schwann and glial cells in regenerated nerves. This study strongly supports the feasibility of using artificial nerve grafts for peripheral nerve regeneration by bridging large defects in a rat model.

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“…Since the nerves connect the brain and spine to the other parts of the body, and thus control sensory and motor functions, [128] nerve damage can lead to the loss of these vital functions. [101] One approach to mitigate the consequences of nerve damage is the autograft technique, but its applicability is limited because it can lead to morbidity, neuroma formation, and the loss of sensation at the nerve harvest site.…”

Section: Neural Repair and Regenerationmentioning

confidence: 99%

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

Boni

Bakadia

Osi

et al. 2021

Macromolecular Bioscience

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The unique properties of silk proteins (SPs), particularly silk sericin (SS) and silk fibroin (SF), have attracted attention in the design of scaffolds for tissue engineering over the past decades. Since SF has good mechanical properties, while SS displays bioactivity, scaffolds combining both proteins should exhibit complementary properties enhancing the potential of these materials. Unfortunately, SS-SF composites can generate chronic immune responses and their immunogenic element is not completely clear. The potential of SS-SF composites in tissue engineering, elements which may contribute to their immunogenicity, and alternatives for their preparation and design, to modulate the immune response and take advantage of their useful properties, are discussed in this review. It is known that SS can enhance 𝜷-sheet formation in SF, which may act as hydrophobic regions with a strong affinity for adsorption proteins inducing the chronic recruitment of inflammatory cells. Therefore, tailoring the exposure of hydrophobic regions at the scaffold surface should represent a viable strategy to modulate the immune response. This can be achieved by coating SS-SF composites with SS or other hydrophilic polymers, to take advantage of their antibiofouling properties. Research is still needed to realize the full potential of these composites for tissue engineering.

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Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide

Cited by 10 publications

References 47 publications

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

In vivo assessment of a nanofibrous silk tube as nerve guide for sciatic nerve regeneration

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

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