Investigation of protein distribution in solid lipid particles and its impact on protein release using coherent anti-Stokes Raman scattering microscopy

Christophersen, Philip C.; Birch, Ditlev; Saarinen, Jukka; Isomäki, Antti; Nielsen, Hanne Mørck; Yang, Mingshi; Strachan, Clare J.; Mu, Huiling

doi:10.1016/j.jconrel.2014.10.023

Cited by 20 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protein-protein interactions for LasR proteins-representatives of LuxR family with bacterial quorum sensing modulators-were characterized by SERS at low~0.5 nanomolar concentrations of the protein [96]. Protein-lipid interactions were analyzed by the CARS approach as a complementary technique used with TEM and SEM that allowed for characterizing protein lysozyme distribution in solid lipid microparticles [97].…”

Section: Rs Of Proteins Protein Interactions and Dynamicsmentioning

confidence: 99%

See 1 more Smart Citation

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

View full text Add to dashboard Cite

This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

show abstract

Section: Rs Of Proteins Protein Interactions and Dynamicsmentioning

confidence: 99%

“…Protein-lipid interactions were analyzed by the CARS approach as a complementary technique used with TEM and SEM that allowed for characterizing protein lysozyme distribution in solid lipid microparticles [97].…”

Section: Rs Of Proteins Protein Interactions and Dynamicsmentioning

confidence: 99%

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Christophersen et al26., 27. investigated that the SLPs with different types of lipid excipients exhibited a lipase-mediated degradation mechanism on release of proteins. He and his colleagues further proved that lysozyme incorporated SLPs in an aqueous solution released lysozyme much faster than in a solid.…”

Section: Lipid-based Particlesmentioning

confidence: 99%

Multifunctional oral delivery systems for enhanced bioavailability of therapeutic peptides/proteins

Han

Gao

Chen

et al. 2019

Acta Pharmaceutica Sinica B

113

View full text Add to dashboard Cite

In last few years, therapeutic peptides/proteins are rapidly growing in drug market considering their higher efficiency and lower toxicity than chemical drugs. However, the administration of therapeutic peptides/proteins is mainly limited in parenteral approach. Oral therapy which was hampered by harsh gastrointestinal environment and poorly penetrating epithelial barriers often results in low bioavailability (less than 1%–2%). Therefore, delivery systems that are rationally designed to overcome these challenges in gastrointestinal tract and ameliorate the oral bioavailability of therapeutic peptides/proteins are seriously promising. In this review, we summarized various multifunctional delivery systems, including lipid-based particles, polysaccharide-based particles, inorganic particles, and synthetic multifunctional particles that achieved effective oral delivery of therapeutic peptides/proteins.

show abstract

“…CARS microscopy based single frequency imaging has been performed on a variety of other pharmaceutical products including protein loaded lipid solid particles [104] and loaded silica particles [105], lipid based solid dosage forms [106] and micronized particles in liquid dosage forms [107]. As has been explained, the fast imaging capabilities of non-linear Raman microscopy is among other factors owed to the reduced spectral information collected, but new developments in the field of broadband CARS microscopy also allow for rapid image acquisition over extended spectral ranges, as was demonstrated in similar experiments.…”

Section: Pharmaceutical Compoundsmentioning

confidence: 99%