Investigation of several chromatographic approaches for untargeted profiling of central carbon metabolism

Girel, Sergey; Guillarme, Davy; Fekete, Szabolcs; Rudaz, Serge; González‐Ruiz, Víctor

doi:10.1016/j.chroma.2023.463994

Cited by 7 publications

(2 citation statements)

References 39 publications

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“…As can be seen from Figure 1, the performance of the HSS T3 column shows favorable sensitivity and efficiency in the analysis of interest, while the retention of ibotenic acid and muscimol on this column is slightly insufficient. The CSH PFP column, with a weak positive charge on the stationary particles, has shown exceptional peak shapes and homogeneous distribution of analyte peaks in various bioanalytical applications and metabolomics [30]. However, in this study, the performance of this column shows deficiencies in either peak shape or response.…”

Section: Optimization Of Lc Columnsmentioning

confidence: 70%

Simultaneous Determination of Multi-Class Mushroom Toxins in Mushroom and Biological Liquid Samples Using LC-MS/MS

Lu,

Zhang,

et al. 2024

Separations

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A comprehensive analytical method based on liquid chromatography–tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous detection of 12 mushroom toxins (ibotenic acid, muscimol, muscarine, β-amanitin, α-amanitin, desoxoviroidin, γ-amanitin, phallisacin, illudin S, phallacidin, phalloidin and illudin M) in mushrooms, serum, urine and simulated gastric fluid. The samples were extracted with water or acetonitrile solution, and the serum sample was further purified with PSA sorbent. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 column with gradient elution using methanol and water containing 1 mM ammonia fluoride as a mobile phase. Mass spectrometric acquisition was performed in electrospray positive ionization mode. Good linearities (R2 > 0.994) were obtained for 12 toxins over the range of 0.05~200 µg/L. Matrix-matched calibration curves were used for quantification. The method limits of quantification were 0.01~0.2 mg/kg for mushrooms and 0.15~2.0 µg/L for three biological liquid samples. The mean recoveries of 12 target toxins (spiked at three concentration levels) ranged from 73.0% to 110.3%, with relative standard deviations not exceeding 19.4%, which meets the requirements for the determination of trace compounds in a biological matrix. This method was applied to the analysis of mushroom samples from Yunnan Province. As a result, 11 toxins, not including illudin M, were detected with a concentration range of 0.61~2143 mg/kg.

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Section: Optimization Of Lc Columnsmentioning

confidence: 70%

Simultaneous Determination of Multi-Class Mushroom Toxins in Mushroom and Biological Liquid Samples Using LC-MS/MS

Lu,

Zhang,

et al. 2024

Separations

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show abstract

“…1–8 The major analytes can be categorised as related to central carbon metabolism (energy metabolism) or metabolic pathways involving molecules such as amino acids, nucleic acids, and lipids. 9–12 This technique, which can provide insights into a broad range of reactions in various research areas, is of interest to not only major companies but also small- and medium-sized companies. Nevertheless, such advanced instrumentation is expensive and difficult to introduce into small- and medium-sized companies.…”

Section: Introductionmentioning

confidence: 99%