Investigation of Skin Graft Viability With DMEM and Ham???s F-12 Fluids

Tosun, Zekeriya; Tm, Aktan; Hoşnuter, Mübin; Savacı, Nedim; Duman, Salih

doi:10.1097/00000637-200108000-00026

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…Moreover, in the case of PBS it was attributed to the saline that keeps preserving the sample. It was reported that various media like DMEM, PBS, Ham's F-12 and other medium have been used in the past as preservation media in skin grafts (Tosun et al, 2001;De et al, 2008). In addition, human skin samples can be conserved in saline solutions (Cetin et al, 2000) or in culture medium (Rosenquist et al, 1988) for 30 days at 4°C.…”

Section: Resultsmentioning

confidence: 99%

Effect of storage media and time on fin explants culture in the goldfish, Carassius auratus

Choresca¹

2012

Afr. J. Biotechnol.

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The effect of storage media and time was investigated on fin explants culture in the goldfish (Carassius auratus). Fin explants under sterile conditions were able to produce cells at different storage media and time. On the outgrowth of cells, fin explants stored for seven days before culturing showed significantly higher growth (P<0.05) as observed on the fin explants stored in Dulbecco's modified Eagles medium (DMEM, 84.44%), phosphate buffered saline (PBS, 62.42%) and in control/fresh fin explants (100%), compared with the explants with no storage medium (25.56%) at day three of culture. From day seven to 14, all caudal fin explants exhibited a 100% outgrowth of cells regardless of treatment. However, caudal fin explants kept for 10 days in 4°C showed a significant difference (P<0.05) from fin explants stored in DMEM (96.67%), PBS (75.57%) and the control (100%), as compared with no storage medium (no growth) on day three. Moreover, only 6.67% of no storage medium group could maintain outgrowth of cells, while other treatment group reached 100% of outgrowth after 14 days of culture.

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Section: Resultsmentioning

confidence: 99%

Effect of storage media and time on fin explants culture in the goldfish, Carassius auratus

Choresca¹

2012

Afr. J. Biotechnol.

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“…Roswell Park Memorial Institute solution, University of Wisconsin solution, Hartmann’s solution, Dulbecco’s modified Eagle’s medium, Marshall’s solution, McCoy’s 5A medium, keratinocyte nutrient MCDB 153, histidine–tryptophan–ketoglutarate solution, or amniotic fluid are other clinically and experimentally used storage media. 7 -9 Cell culture media at 4°C are generally superior to saline in extending tissue survival time. Aside from refrigeration at 4°C in storage medium, there are 2 alternative ways to prepare and store skin samples—one is by freezing after cryopreservation with glycerol; the other is storage at room temperature after freeze-drying.…”

Section: Discussionmentioning

confidence: 99%