Investigation of Stress Situation of Pediatric Nurses and Corresponding Changes of Intestinal Flora

Abstract

BACKGROUND: Sequencing the stool samples to explore the relationship between intestinal flora and the stressor of nurses. METHODS: The research take the pediatric nurses in the Third Xiangya Hospital as the research group and the internal medicine nurses as the control group. Use the "Chinese Nurse Stressor Scale"(CNSS) for general investigation and stress-related assessment. 6 in internal medicine group and 7 in pediatric group to conduct cell sequencing of intestinal flora. Gut microbiome was profiled by 16S rRNA sequencing. RESULTS: The work stressors of internal medicine and pediatric nurses includes nursing specialty and work (10.94±3.17 vs 12.25±2.78), work environment and resources (4.91±1.63 vs 5.66±2.03), patient care (15.86±4.30 vs 18.95±5.12) and management and interpersonal relationship (8.83±4.94 vs 10.86±5.01). The results were statistically different (P < 0.05). The abundance of Clostridia in internal medicine group is higher, while the abundance of Bacteroidia in pediatric group is higher. The abundance of γ-proteobacteria and β-proteobacteria also have obvious differences between two groups.CONCLUSIONS: The overall stress score of the pediatric nurse group was higher, with their stool samples having more abundance of Bacteroidia, but Clostridia, γ-Proteobacteria and β-Proteobacteria are fewer than that in internal medicine nurses.