Investigation of structural changes of β-casein and lysozyme at the gas–liquid interface during foam fractionation

Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin

“…1. It is presented that protein molecules suffer structural unfolding as they are adsorbed at the gas–liquid interface [1]. Because a large gas–liquid interfacial area is used in foam fractionation, the number of protein molecules that suffer denaturation at the interface will be large.…”

Prevention of irreversible aggregation of whey soy proteins in their foam fractionation from soy whey wastewater

“…In the rising foam, the WSP molecules were distributed at the gas-liquid interface and in the entrained liquid between bubbles. The adsorbed WSP molecules had more unfolded structure than those in the entrained liquid [8] so that they more readily interacted with each other in the desorption process. According to the principle of foam fractionation, a high enrichment ratio meant a high ratio of the amount of WSP adsorbed at the gas-liquid interface vs the amount of WSP in the entrained liquid.…”

“…It is well known that the protein molecules adsorbed at the gas-liquid interface suffer structural unfolding to make hydrophobic groups exposed to the gas phase. [8] The unfolded molecules readily interact with each other via hydrophobic interaction, so protein aggregates are readily formed at the gas-liquid interface or in the desorption of protein molecules from the interface. [9] At present, no strategies are reported to prevent the irreversible aggregation of WSP in foam fractionation, although many efforts have been made to improve the separation performances.…”

Thermodynamic adsorption properties of bovine serum albumin and lysozyme on the bubble surface from the binary solution

“…So, there are mixtures of proteins and enzymes. BSA and LZM are often used as a simulation protein and a simulation enzyme, respectively in bioseparation, especially in foam fractionation (Barackov et al, 2012;James et al, 1991;Mitropoulos et al, 2014). In our work, BSA represented a protein and LZM represented an enzyme.…”