Investigation of surgical adhesives for vocal fold wound closure

Abstract

Objectives Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure or reduce scarring. The objectives of this study were to investigate the mechanical strength and the cytotoxicity of three commercially available adhesives (Glubran 2, GEM, Viareggio, Italy; BioGlue, CryoLife, Kennesaw, GA; and Tisseel, Baxter Healthcare, Deerfield, IL) for vocal fold wound closure. Methods Shear and tension tests were performed on 150 porcine larynges. The cytotoxicity of the adhesives to immortalized human vocal fold fibroblasts was investigated using neutral red uptake assays. Results The average shear adhesive strength for Tisseel, BioGlue, and Glubran 2 was 13.86 ± 5.03 kilopascal (kPa), 40.92 ± 17.94 kPa, and 68.79 ± 13.29 kPa, respectively. The tensile adhesive strength for Tisseel, BioGlue, and Glubran 2 was 10.70 ± 6.42 kPa, 34.27 ± 12.59 kPa, and 46.67 ± 12.13 kPa, respectively. The vocal fold cell viabilities in extracts of Tisseel, BioGlue, and Glubran 2 were 99.27%, 43.05%, and 1.79%, respectively. Conclusion There was a clear tradeoff between adhesive strength and toxicity. The maximum failure strength in shear or tension of the three surgical adhesives ranked from strongest to the weakest was: 1) Glubran 2, 2) BioGlue, and 3) Tisseel. Tisseel was found to be the least toxic of the three adhesives, whereas Glubran 2 was the most toxic. Level of Evidence NA Laryngoscope, 129:2139–2146, 2019

An Injectable, Shape-Retaining Collagen Hydrogel Cross-linked Using Thiol-Maleimide Click Chemistry for Sealing Corneal Perforations

“…The lap shear strengths were 8.5 ± 2.8 kPa for thiol collagen hydrogel and 8.3 ± 1.3 kPa for fibrin glue (Figure a). The lap shear strength values obtained here for fibrin glue are in good agreement with earlier reports. − Moreover, the failure profile of the developed hydrogels was found to be very similar to that of the fibrin glue (Figure b). A gradual decrease of the lap shear stress after passing the maximum value was observed for both adhesives resulting in a large area under the curve, indicating a ductile nature of both adhesives .…”

The Effects of Using Bioglue in Nasal Septal Surgery

“…However, an unacceptably high incidence of cerebrospinal fluid leakage (62.5%) was determined and ıt was recommended that Bioglue is not used in translabyrinthine vestibular schwannoma surgery. Chen et al 45 used Bioglue for vocal fold wound closure. They compared Tisseel, Bioglue, and Glubran 2 surgical adhesives for adhesive strength and the cytotoxicity.…”

In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium

“…Type-I collagen is the most widely used in tissue engineering and is generally used as a scaffold of a component material for maintaining or transferring the extracellular matrix structure (24)(25)(26). Fibrin glue is also a clinically-adopted bioadhesive (33,34,40,41). These scaffolds are clinically useful tools, are less expensive than Matrigel, and have been proven to be safe when locally administered.…”