Investigation of tetracycline residues in fish caught from surrounding fish farms in Mugla district

Abstract

ÖzAmaç: Su ürünlerindeki ilaç ve metabolitlerinin kalıntı analizleri Ulusal Kalıntı İzleme Planı kapsamında düzenli olarak yapılmaktadır. Fakat Türkiye'de bu kalıntıların sucul ortama etkilerine yönelik araştırmalar sınırlıdır. Bu çalışmada, Muğla bölgesindeki balık çiftliklerinin etrafından avlanan balıklarda tetrasiklin grubu antibiyotiklerin (oksitetrasiklin, tetrasiklin, klortetrasiklin, doksisiklin) kalıntılarının belirlenmesi amaçlandı. Gereç ve Yöntem:Analiz için 70 ayrı çiftlik etrafından toplanan 70 adet balık örneği kullanıldı. Balıklarda tetrasiklin kalıntıları LC MS/MS yöntemi ile analiz edildi. Seçicilik, doğ-rusallık, geri kazanım, duyarlılık ve kesinlik ölçütlerinde metot validasyonu yapıldı. Bulgular:Analizin geri kazanımları oksitetrasiklin için %100.5, tetrasiklin için %101.3, klortetrasiklin için %99 ve doksisilin için %100.5 olarak belirlendi. Analizlerin sonucunda balık örneklerinin hiçbirinde tetrasiklin grubu antibiyotikler tespit edilemedi.Öneri: Yapılan analizlerde balık örneklerinde hesaplanabilir limitlerde tetrasiklin kalıntısı bulunmaması çevre ve halk sağlığı açısından memnuniyet verici olarak kabul edilebilir. Anahtar kelimeler: Balık, tetrasiklin, kalıntı, LC MS/MS AbstractAim: Residue analyses of drugs and metabolites in the aquaculture are regularly conducted under the National Residue Monitoring Plan in Turkey. However, the research on the impact of these residues on the aquatic environment is limited. In this study, it was aimed to analyze the residues of tetracycline group antibiotics in fishes caught around fish farms in the Muğla district. Materials and Methods:Seventy fish samples collected from the vicinity of 70 different fish farms were used for analysis. Tetracycline residues in the fish were analyzed with the LC MS/MS method. Validation was performed with the criteria of specificity, linearity, recovery, precision and sensitivity.Results: Recovery values were determined as follows: Oxytetracycline 100.5%, tetracycline 101.3%, chlortetracycline 99%, and doxycycline 100.5%. At the end of the analyses, no tetracycline antibiotic residues were found. Conclusion:It can be expressed that no tetracycline residues were detected in the samples at the detectable limits is a satisfactory result in terms of public health and environment.

Oxytetracycline residue in Tilapia

“…They observed that 63.1% of the samples contained the residues of tetracyclines where maximum samples contained residues at a range of 1.43 -91.130 ppb and only one sample (101.40 ppb) exceeds the maximum residue limit of oxytetracycline given by the European Commission. A recent study of Turk and Oguz (2016) observed residues of tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in 70 fish samples from 70 different fish farms around Muğla province, Turkey. At the end of the analyses, no tetracycline antibiotic residues were found crossing the detection limit.…”

Effects of oxytetracycline residues in Thai Koi (Anabas testudineus Bloch) collected from Sylhet, Bangladesh

“…Where they reported at measurable levels 63.1% of the samples were contained with the residues of tetracyclines in which maximum sample contained residues at a range 1.43 -91.130 ppb and only one sample (101.40 ppb) exceeds the maximum residue limit of oxytetracycline set by the European Commission. Turk and Oguz (2016) demonstrated a research to observe the residual effects of tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in 70 fish samples from 70 various fish farms in Mugla province of Turkey. No tetracycline antibiotic residues were found, cross the detection limit.…”

Oxytetracycline residues in Thai pangas Pangasianodon hypophthalmus sampled from Sylhet sadar upazila, Bangladesh

“…Mahmoudi et al (2014) also reported 8.44±6.03 ppb tetracyclines residues in Oncorhynchus mykiss and the confined tetracyclines residues were lower than the detection limit of present study. Turk and Oguz (2016) found no oxytetracycline in fish samples collected from Muğla province, Turkey. Shim et al (2010) also reported no oxytetracycline residues in some examined fish during their study.…”