Investigation of the Anti-Tumour Action of Eupatoriopicrin Against the Lewis Lung Tumour

Abstract

In this work eupatoriopicrin, the principal sesquiterpene lactone in Eupatorium cannabinum L., was tested for possible cytostatic activity in an experimental solid tumour system of the mouse: the Lewis lung tumour. The tumour cells were transplanted subcutaneously in the left back flank of female C57BL mice. Eupatoriopicrin was administered intraperitoneally as a fine suspension in saline. The animals were treated with the drug at a tumour volume of 500 tl or at an earlier stage, at which no tumour was visible yet. After treatment, progress of tumour growth was measured and compared with a control group. Different doses and dosage schedules were used. Treated animals showed a delay in tumour growth. Repeated administration resulted in a prolonged growth delay.

Chemical-Microbiological Synthesis of Cryptomeridiol Derivatives by Gliocladium roseum: Semisynthesis of 11-Hydroxyeudesmanolides

“…19 Sesquiterpene lactones show a wide variety of remarkable biological activities, but many of them present strong cytotoxic properties, apparently due to the R, -unsaturated lactone group. [20][21][22][23] Some authors suspect that 11-hydroxysesquiterpenolides are the precursors of those compounds, and thus the unsaturated system could be released in small quantities, thereby attenuating the cytotoxic effect. 24 In previous papers 25,26 we reported the incubations of several 4 -hydroxyeudesmane compounds by the filamentous fungi Curvularia lunata ATCC 12017 and Rhizopus nigricans ATCC 10404.…”

Sesquiterpenoids Isolated from Eupatorium glehnii. Isolation of Guaiaglehnin A, Structure Revision of Hiyodorilactone B, and Genetic Comparison

“…Hiyodorilactone B (6) showed slightly higher activity (IC 50 of 1.95 mM). Eupatoriopicrin (18) 5,6,[36][37][38][39] isolated from the Hokkaido sample was the most effective compound (IC 50 of 1.3 mM) among those tested. We have previously investigated the chemical constituents of E. fortunei, 40) but only derivatives of aromatic substance were isolated.…”

Seven Germacranolides, Eupaglehnins A, B, C, D, E, and F, and 2&alpha;-Acetoxyepitulipinolide from <i>Eupatorium glehni</i>

“…Examples of chlorine atom-containing substances have been reported, although not very often, in terrestrial plants. [20][21][22][23] In our attempt to find cytotoxic compounds, eupatoriopicrin (8) [24][25][26][27][28][29] was the most effective (1.40 mg/ml), followed by eupaglehnin C (3) (2.19 mg/ml) against HeLa-S3. However, eupaglehnins E (5) and F (6) did not show cytotoxic activity, which is understandable because they had no exomethylene group.…”