This study characterizes the 3D printed transpiration cooling (TC) channels in a single-point cutting tool to enhance the lubrication and cooling at the cutting zones. Five different TC channels namely two circular profiled channels (Design 1-A & 1-B), one hexagonal (Designs 2-A & 2-B) profiled channels, one bio-inspired blood vessel (Designs 3) are designed inside a single-point turning tool and 3D printed using AISI-1.2709 in powder bed fusion (PBF). From the materials and mechanical characterisation, fine cellular microstructure and high hardness is achieved. X-ray micro computed tomography (XRµCT) has been used as a non-destructive inspection strategy to analyse the built structures. The results of XRµCT showed that the TC channel built is highly orientation dependant, steeper angles deviates highly and nominal angles such as 0º and 90º (to the build platform) provides the best dimensional accuracy. The average dimensional deviations of the five designs are -35.8%, -19.42%, -19.45%, -15.85%, and -5.02%, respectively from the as-designed. The best designs are circular free-form (Design 1-B), hexagonal free-form (Design 2-B) and bio-inspired blood vessel (Design 3), which has the least dimensional deviation and highest accuracy.