Investigation of the effect of astaxanthin on alveolar bone loss in experimental periodontitis

Yüce, Hatice Balcı; Alpan, Aysan Lektemür; Gevrek, Fıkret; Toker, Hülya

doi:10.1111/jre.12497

Cited by 34 publications

(22 citation statements)

References 43 publications

(105 reference statements)

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“…Regarding the inflammatory cells, fibroblast cells and endothelial cells could easily be differentiated from inflammatory cells which were macrophage, neutrophil, eosinophil, T lymphocytes, and plasma cells . Fibroblasts have a thin and long spindle‐like shape, while other cells have a more rounded shape . The cells which were not easily differentiated were not counted.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Taşkan

Yüce

Karataş

et al. 2019

J of Periodontal Research

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The Objective The present study aimed to evaluate the effects of oleuropein on ligature‐induced alveolar bone loss. In this respect, osteoblastic activity, osteoclastic activity, inflammatory markers, and apoptosis were evaluated. Background Oleuropein is a flavonoid, which has potent anti‐inflammatory and bone‐protective effects. Methods Thirty‐two Wistar rats were divided into four experimental groups as following: control (C, n = 8) group; periodontitis (P, n = 8) group; periodontitis and low‐dose oleuropein group (12 mg/kg/day oleuropein, LDO group, n = 8); and periodontitis and high‐dose oleuropein group (24 mg/kg/day oleuropein, HDO group, n = 8). Periodontitis was induced via ligatures. Study period was 14 days, and animals were sacrificed at end of this period. Mandibles were examined via a stereomicroscope and underwent histological procedures. Osteoblast, tartrate‐resistant acid phosphatase (TRAP)‐positive osteoclast, and inflammatory cell counts were determined in hematoxylin‐eosin stained sections. Inducible nitric oxide synthase (iNOS), bone morphogenetic protein‐4, the cluster of differentiation (CD)‐68, cysteine‐aspartic proteases‐3 (Caspase 3), and B‐cell lymphoma‐2 (Bcl‐2) expressions were evaluated via immunohistochemistry. Results Periodontitis group had highest alveolar bone loss, and these levels significantly decreased in LDO and HDO groups. Both 12 and 24 mg/kg oleuropein groups significantly increased osteoblast cell counts and decreased TRAP‐positive osteoclast and inflammatory cell counts. BMP‐4 and bcl‐2 expressions were elevated in oleuropein groups while caspase‐3 expressions decreased. iNOS and CD68 were higher in periodontitis group compared to control group, but there was no significant difference between other groups. Conclusion Oleuropein successfully decreased alveolar bone loss as a result of decreased osteoclastic activity, inflammation, and apoptosis and increased osteoblastic activity.

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Section: Methodsmentioning

confidence: 99%

“…For the osteoblast cells, periodontal ligament neighboring alveolar bone to observe the lining surface of the alveolar bone was evaluated. Measurements were performed at three different sites, mesial‐coronal, apical, and distal‐coronal, and a mean value was recorded …”

Section: Methodsmentioning

confidence: 99%

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Taşkan

Yüce

Karataş

et al. 2019

J of Periodontal Research

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“…Experimental periodontitis model was induced via ligation method that is one of the most common methods for periodontitis induction . ‒ In this method, sutures around teeth cause dental plaque deposition, gingival inflammation, and alveolar bone loss which has a more rapid and acute course compared with human form of the disease . ‒ However, the advantages of the method such as similarity to humans in anatomic structure and response to therapy, cost of the experimental procedure, easy handling, and availability, make it a desired technique for periodontal disease induction .…”

Section: Discussionmentioning

confidence: 99%

“…Staining was performed to identify active osteoclasts which produce TRAP enzyme . ‒ Sections were rehydrated with ethanol and placed into 0.2‐M acetate buffer (consisting of 0.2‐M sodium acetate and sodium tartrate dibasic dehydrate) for incubation 20 minutes at room conditions. After incubation, sections were subjected to naphthol AS‐MX phosphate and fast red TR salt, which were added to the acetate buffer solution, for 45 minutes at 37°C.…”

Section: Methodsmentioning

confidence: 99%

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The effect of luteolin in prevention of periodontal disease in Wistar rats

Yüce

Toker

Yıldırım

et al. 2019

Journal of Periodontology

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Background Periodontal disease is the chronic infectious disease of the periodontium. Because of irreversibility, prevention of disease is one of the most important goals of periodontal treatment. The aim of this study was to evaluate the effect of luteolin, a powerful anti‐inflammatory agent, on the prevention of experimental periodontitis by determining morphological and histological tissue alterations. Methods This study consisted of 28 rats and four experimental groups: healthy control group (C, n = 6); periodontitis group (P, n = 6); periodontitis and 50 mg/kg luteolin administered group (L‐50, n = 8); and periodontitis and 100 mg/kg luteolin administered group (L‐100, n = 8). Experimental periodontitis was induced via ligature method around lower right first molar teeth. All rats were euthanized 11 days after. The severity of periodontal destruction was determined by measuring alveolar bone loss under a stereomicroscope. Osteoblast and inflammatory cell counts were counted on hematoxylin‐eosin‐stained slides and osteoclasts were counted on tartrate‐resistant acid phosphatase‐stained slides. The levels of inducible nitric oxide synthase (iNOS), bone morphogenetic protein (BMP)‐2, matrix metalloproteinase (MMP)‐8, tissue inhibitor of MMP (TIMP)‐1, receptor activator of nuclear factor κB ligand (RANKL), and osteoprotegerin (OPG) were determined by immunohistochemistry. Results The highest alveolar bone loss was observed in the periodontitis group and the luteolin administration decreased bone loss in both groups. Osteoblast cell number was higher and osteoclast and inflammatory cell numbers were lower in the P group compared to C, L‐50, and L‐100 groups. Luteolin, dose‐dependently increased osteoblast cell counts. Luteolin attenuated periodontal inflammation in both L‐50 and L‐100 groups. Like osteoblast cell numbers, BMP‐2 expressions were also elevated in luteolin groups. Both doses of luteolin significantly increased TIMP‐1 and BMP‐2 expressions and decreased MMP‐8 levels. iNOS expressions increased in P group and L‐100 significantly decreased iNOS levels. RANKL increased and OPG decreased in P group and 100 mg/kg luteolin increased OPG and decreased RANKL levels significantly. Conclusions Within the limits of present experimental study, luteolin successfully improved periodontal health in a ligature‐induced experimental periodontitis model in Wistar rats. The decrease in inflammation, osteoclastic and collagenase activity and increase in osteoblastic activity are possibly involved in this process.

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Mechanistic role of astaxanthin derived from shrimp against certain metabolic disorders

Yaqoob

Arshad

Imran

et al. 2021

Food Science & Nutrition

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Metabolic syndrome are a cluster of metabolic disorders in the same individual. The primary agreement concerning the description of the metabolic syndrome was established by the International Diabetes Federation (IDF) in the year of 2005. According to IDF, the main risk factor is obesity which is calculated based on body mass index (BMI) and waist circumference. The additional factors for the diagnosis of metabolic syndrome are reduced high-density lipoprotein (HDL) cholesterol, fasting plasma glucose, triglycerides, or blood pressure-. The patho physiology of metabolic syndrome is very composite and is not clearly established. The prevalence of metabolic syndrome is high among those individuals who are older, obese, having insulin resistance, and living a sedentary life style. The main risk factors are excess caloric intake, genetics, weight, lifestyle, and aging (Bonomini et al., 2015).Metabolic syndrome and the risk factors associated with this syndrome such as atherosclerosis, hypertension, dyslipidemia, and hyperglycemia are the main reasons for increasing medical issues in industrialized countries. The main risk factors for rising metabolic syndrome are physical immobility and a diet high in carbohydrates and fats (O'Neill & O'Driscoll, 2015). Oxidative stress is the main reason that causes metabolic syndrome (Furukawa et al., 2017).Oxidative stress leads elevationed intracellular stage reactive oxygen species (ROS) that causes damage to DNA, proteins, and lipids.Oxidative stress is associated with numerous pathologies (Schieber

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Investigation of the effect of astaxanthin on alveolar bone loss in experimental periodontitis

Abstract: Within the limits of this study, it can be suggested that astaxanthin administration may reduce alveolar bone loss by increasing osteoblastic activity and decrease osteoclastic activity in experimental periodontitis model.

Cited by 34 publications

References 43 publications

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

The effect of luteolin in prevention of periodontal disease in Wistar rats

Mechanistic role of astaxanthin derived from shrimp against certain metabolic disorders

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