Investigation of the effects of propofol and vitamin C administration on erythrocyte deformability in rats with streptozotocin-induced

Abstract

Abstract:Purpose: In the current study we aim to investigate the effects of vitamin C and profol on red blood cell deformability in diabetic rats Materials and methods: Twenty-eight Wistar Albino rats were included in the study after streptozocin (60 mg/ kg) treatment for 4 weeks of observation for diabetes presence. Twenty-eight rats were allocated to 4 groups. In group DP (n = 7) 150 mg.kg -1 of propofol was injected intraperitoneally. In group DP-vit C (n = 7) rats 100 mg/kg of vitamin C (Ascorbic acid, Redoxon ® 1000 mg/5 mL -Roche) were applied one hour before administrating 150 mg.kg -1 of propofol, while rats in control group (n = 7), and diabetic control group (n = 7) received intraperitoneally physiological saline. Deformability measurements were achieved by using erythrocyte suspensions with hematocrit level of 5 % in PBS buffer. Results: Erythrocyte deformability was signifi cantly higher in diabetic control group than in control and vitamin C plus propofol groups (p = 0.00, p = 0.025, respectively). Erythrocyte deformability indexes were found similar in control group and vitamin C plus propofol group (p = 0.949). Relative resistance was increased in diabetic rat model. Conclusions: Erythrocyte deformability was damaged in rats with diabetes. This injury might lead to further problems in microcirculation. Application of propofol did not alter red cell deformability in diabetic rats. Vitamin C supplementation seems to reverse those negative effects and variations in erythrocyte deformability (Fig. 2

The Role of Oxidative Stress and Antioxidants in Diabetes Mellitus

“…Diyabetik ratlara 200mg/kg dozda E vitamini takviyesi yapıldığında plazma glukoz düzeyinin azalmadığı ancak MDA düzeyinin azaldığı ve antioksidan enzimlerle (CAT, GSH-PX, GSH-RD) antioksidan (E vitamini, C vitamini, ürik asit, eritrosit glutatyon düzeylerinin) normal seviyede olduğu tespit edilmiştir (53). Bir başka çalışmada diyabetik ratlara C vitamini verildiğinde eritrosit deformasyonunun düzeldiği görülmüştür (54). Melatoninle birlikte C ve E vitamini verilen diyabetik ratlarda plazma glukoz ve MDA düzeyleri azalmış, hematolojik ve biyokimyasal parametrelerle antioksidan düzeylerinin normale döndüğü görülmüştür (55).…”

Effects of apelin-13 on myocardial ischemia reperfusion injury in streptozotocine induced diabetic rats

“…In the past 20 or 30 years the prevalence of diabetes mellitus (DM) has rapidly increased throughout the world, and experts estimate that it is going to increase by 200 % in the next several decades (1).…”

“…Margolis et al showed that the rate of unrecognized myocardial infarction was 39 % in diabetic patients while in non-diabetic patients it was 22 % (3). Cardiac surgery with cardiopulmonary bypass inevitably causes a systemic infl ammatory response and ischemia-reperfusion (I/R) injury affecting multiple organs (1). Several drugs have been used to prevent I/R injury, including Vitamin C, levosimendan, and dexmedetomidine (1,4,5).…”

Effect of apelin-13 on erythrocyte deformability during ischaemia–reperfusion injury of heart in diabetic rats

“…In last two or three decades, the prevalence of diabetes mellitus (DM) has rapidly increased throughout the world, and experts estimate that it will increase by 200 % in the next several decades (1). Erythrocyte deformability (ED) facilitates blood fl ow through the circulation in vessels of variable diameter and enables effective exchange of gas and metabolic products in capillaries (2).…”

“…One study demonstrated that the rate of unrecognised myocardial infarction was 39 % in diabetic patients and 22 % in non-diabetic patients (9). Cardiac surgery with cardiopulmonary bypass inevitably causes a systemic infl ammatory response and ischaemia-reperfusion (IR) injury affecting multiple organs (1).…”