Investigation of the effects of unilateral total salpingectomy on ovarian proliferating cell nuclear antigen and follicular reserve: experimental study

Atılgan, Remzi; Kuloğlu, Tuncay; Boztosun, Abdullah; Orak, Uğur; Başpınar, Melike Mercan; Can, Behzat; Sapmaz, Ekrem

doi:10.1016/j.ejogrb.2015.02.028

Cited by 18 publications

(21 citation statements)

References 23 publications

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“…H&E staining was applied on every 10 th section of the ovary so that each section was separated by a distance of approximately 50-60 μm from the next 10 th section to evaluate the ovarian structure and follicle count [20]. Ovarian follicles were counted and classified according to the fol-lowing follicle type: primordial follicle, primary follicle, secondary follicle, Graafian (mature) follicle [21]. All sections were evaluated with an optical microscope (Olympus CX41, Tokyo, Japan ) and monitored with cellSens digital imaging software (Ver.…”

Section: Histological Analysis and Follicle Countmentioning

confidence: 99%

Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study

Köle

Özkan

Eraldemır

et al. 2020

aoms

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The aim of the study was to investigate whether melatonin has a protective effect against diminished ovarian reserve induced by smoking. Material and methods: Seventy-two female Wistar-Albino rats were divided into 6 groups: group I (room air), group II (chronic cigarette smoking), group III (room air + 10 mg/kg subcutaneous melatonin), group IV (room air + 20 mg/ kg subcutaneous melatonin), group V (chronic cigarette smoking + 10 mg/kg subcutaneous melatonin), group VI (chronic cigarette smoking + 20 mg/kg subcutaneous melatonin). For 45 days, rats were exposed to cigarette smoke through a smoking machine, then subcutaneous melatonin was administered. Apoptotic index, immunohistochemical scoring, ovarian follicle counting, ovarian tissue and serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) analyses were carried out. Results: All of the primordial, primary, secondary and mature follicle numbers were found to be significantly lowered in study groups. Increased HSCORE with anti-caspase-3 staining and a high follicular apoptotic index were demonstrated in the smoking group. Serum and ovarian tissue levels of MDA were found to be elevated with smoke exposure whereas lower MDA levels were determined in melatonin treated groups. Serum and tissue levels of SOD, GPx and CAT were shown to be reduced in the smoking group in comparison with melatonin treated and control groups. 20 mg/kg melatonin administration in the smoking group revealed significantly decreased HSCOREs and apoptotic indices. Conclusions: Cigarette smoking has been definitely shown to be associated with impaired ovarian reserve with respect to significantly diminished numbers of primordial, primary, secondary and mature follicles. Dose-related treatment of melatonin in smokers may provide an evidently reduced apoptotic index and improved antioxidant activity in tissue.

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Section: Histological Analysis and Follicle Countmentioning

confidence: 99%

Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study

Köle

Özkan

Eraldemır

et al. 2020

aoms

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“…P. Ulug и G. Oner [16], R. Atilgan и соавт. [10] в экс-перименте на крысах показали снижение тотально-го и функционального овариального резерва через 1 мес после односторонней СЭ. Установлено сниже-ние количества вторичных растущих фолликулов, увеличение числа желтых тел и атретических фол-ликулов на стороне операции по сравнению с кон-тролем.…”

Section: Discussionunclassified

“…В литературе представлены исследования, опи-сывающие отрицательное воздействие СЭ на овари-альный резерв [9][10][11][12][13][14][15][16], косвенные доказательства снижения функции яичников [17] и данные об от-сутствии влияния операции на женские гонады [8,[18][19][20][21][22].…”

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Ovarian reserve after preventive salpingectomy

Petrov¹,

Логвинов²,

Tikhonovskaya³

et al. 2015

Probl. reprod.

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“…Deparaffinized tissues were passed through a graded alcohol series and boiled in citrate buffer solution at pH 6 in a microwave oven (750 W) for 12 min in order for antigen retrieval as defined by Atilgan et al [26]. In order to prevent surface staining, after treatment with Ultra V Block (TA-125-UB, the Lab Vision Corporation, USA) solutions, the tissues were incubated with primary antibodies for 60 min (CHRM1 was purchased from Boster (Cholinergic receptor, muscarinic 1, catalog number: PA2202, Boster, 3942 B Valley Ave, Pleasanton, CA, 94566) and TRPM2 was purchased from Abcam (Rabbit Anti-TRPM2 antibody, ab101738, Abcam, Cambridge, UK)).…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

The decrease in hippocampal transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) is associated with memory loss in a surgical menopause rat model

et al. 2021

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The aim of the study was to investigate the association of transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) activity with the memorial functions that are deteriorated in surgical menopause. Material and methods: A total of 14 female rats were randomly divided into 2 groups: group (G)1: sham group; group (G)2: surgical menopause group, the group in which bilateral ovariectomy was performed. Fourteen days after the surgical procedure, learning and memorial tests were performed in G1 and G2 for a totally 13 days. The time required for the rats to find the cheese in the labyrinth was recorded and statistical evaluation of it was performed between groups. On the 14 th day of the memory test, the rats were decapitated and the brain tissues were fixed in 10% formalin. Hippocampal TRPM2 and CHRM1 gene expression was evaluated with RNA isolation, complementary DNA (cDNA) synthesis and quantitative real-time PCR (qRT-PCR) analysis. TRPM2 and CHRM1 immunoreactivity was evaluated in hippocampal tissue with the immunohistochemical method. Histo-score was calculated regarding the diffuseness of and severity of the staining; and statistical analyses were performed. Results: In the ovariectomized group, the mean time required for the rats to find the cheese was statistically significantly elongated (39.29 ±4.0 s vs. 29.86 ±2.6 s). When the hippocampal TRPM2 and CHRM1 gene expression and immunoreactivity were compared with the sham group, there was a statistically significant decrease in the surgical menopause group (p < 0.05). Conclusions: In surgical menopause, in deterioration of memorial functions, hippocampal TRPM2 channel and CHRM1 activity plays an important role.

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Investigation of the effects of unilateral total salpingectomy on ovarian proliferating cell nuclear antigen and follicular reserve: experimental study

Cited by 18 publications

References 23 publications

Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study

Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study

Ovarian reserve after preventive salpingectomy

The decrease in hippocampal transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) is associated with memory loss in a surgical menopause rat model

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