Investigation of the effects of static magnetic field on apoptosis in bone marrow stem cells of rat

Abstract

This research was carried out to evaluate the influence of static magnetic field on the rate of apoptosis in bone marrow stem cells (BMSCs) of rats. Extracted cells were suspended in aMEM as a culture media for 48 h. After that, cells were exposed to 15 mT static magnetic field (SMF) for 5 h, incessantly with or without FeCl 2 . The rate of apoptosis was then assessed via flow cytometery. The results showed that either treatment with FeCl 2 or exposure to SMF enhanced the rate of apoptotic cells. Moreover, cells that were treated simultaneously with FeCl 2 and SMF have higher rate of apoptosis. An increase in apoptosis by 26.5% was induced by SMF alone and an increase in apoptosis by 28.2% was induced by a combination of FeCl 2 and SMF, compared to their corresponding controls. The results recommended that the effects of SMF on apoptosis may be related to increment of the number of free radicals in the cells.

Static Magnetic Field Effect on Cell Alignment, Growth, and Differentiation in Human Cord-Derived Mesenchymal Stem Cells

“…Absence of SMF effect on cell growth include human fetal lung fibroblasts. 56 In contrast other studies detected SMF influence on apoptosis 1,49 and neuron response. 38 Moderate-intensity SMF induced modifications of cell shape, cell surface, and cytoskeleton progressively inflicted during the entire period of exposure.…”

Effect of static magnetic field with quercetin and hesperetin on MCF-7 and MDA MB-231 breast cancer cells

“…SMF administration may reduce the levels of calcium ion via affecting either the characteristics of Ca 2+ -ATPase or altering the role of Ca 2+ binding protein and the ion specific pores [22][23][24]. This hypothesis would mainly define all of the biological properties such as the regulation of apoptosis [25], proliferation and cell viability [26]. Other potential mechanisms of SMF that cause disorganization of the apoptotic rate such as modulation of the gene expressions, production [17,24] or increase of oxygen free radicals were also significant.…”

Anticancer Effects of Moderate Static Magnetic Field on Cancer Cells In Vitro

“…The higher sensitivity of HeLa cells can be attributed to its higher proliferation rate and higher metabolism and also its poorer differentiated than fibroblast cells. Research in Molecular Medicine (RMM) Jaber Zafari et al DNA damage in lymphocytes of patients treated with both iron chloride and 7 mT static magnetic field, which led to cell death through apoptosis or necrosis (29). In a study investigating the simultaneous effects of static magnetic fields and iron ions, the effects of the iron ions alone were also tested.…”