Investigation of the effects of polar organic solvents on the activity of tyrosinase entrapped in a poly(ester-sulphonic acid) polymer

Iwuoha, Emmanuel I.; Adeyoju, Olubunmi; Dempsey, Eithne; Smyth, Malcolm R.; Liu, Jie; Wang, Joseph

doi:10.1016/0956-5663(95)96956-y

Cited by 34 publications

(19 citation statements)

References 16 publications

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“…Several aspects of these data are noteworthy. First, the SPEs coated with AQ polymer with the procedure reported by Iwuoha [17] lost approximately 40% of the initial catechol sensitivity after aging at 65 ЊC for 2 weeks. Because Gibson et al reported that a combination of sugar alcohols and zinc ions stabilized several other enzymes, lactitol and zinc ions were added to the AQ-tyrosinase solution.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the electrodes produced by Kottee et al are not sufficiently stable for commercial applications. Iwuoha, Adeyoju, Dempsey, Liu, and Wang [17] showed that tyrosinase could be immobilized in an ionomer film on the surface of an electrode by a simple inexpensive casting process, but did not apply this process to mass-producible electrodes, nor demonstrate stability sufficient for commercial applications. Recently, Wang [18] demonstrated that inexpensive biosensors for phenolic compounds could be produced by screen-printing a mixture of graphite and tyrosinase in a single step.…”

Section: Contract Grant Sponsor: U S Environmental Protection Agencmentioning

confidence: 99%

“…The working electrodes were coated with the tyrosinase-AQ solution reported by Iwuoha et al [17]. This solution was prepared by heating 2.8% AQ resin in a 0.05-M pH 7.0 phosphate buffer for several minutes until the resin dissolved, cooling the solution, and then adding 1 mg/ml tyrosinase.…”

Section: Screen-printed Electrodesmentioning

confidence: 99%

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Enzyme-based electrodes for environmental monitoring applications

Schmidt¹

1998

Field Analyt. Chem. Technol.

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Section: Resultsmentioning

confidence: 99%

Section: Contract Grant Sponsor: U S Environmental Protection Agencmentioning

confidence: 99%

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Enzyme-based electrodes for environmental monitoring applications

Schmidt¹

1998

Field Analyt. Chem. Technol.

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“…To probe if the biosensor response follows Michaelis -Menten kinetics, the Hill coefficient, n, was calculated [16]. The n values for all biosensors were quite higher than unity, indicating a deviation from Michaelis-Menten kinetics, probably due to the difficulty of the substrate to diffuse through the polymeric matrix, so, catalytic constants could not be calculated.…”

Section: à2mentioning

confidence: 99%

Biosensors Based on Poly(vinylimidazole) Microparticles

2009

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The use of poly(vinylimidazole) microparticles as immobilization system of the enzymes used as biological material in the fabrication of glucose and catechol biosensors is proposed. Biosensors analytical properties were studied using different working media: aqueous buffer solution and solvents with different hydrophobicity such as dioxane (lgP ¼ À 1.1), acetonitrile (lgP ¼ À 0.33), ethanol (lgP ¼ À 0.24), chloroform (lgP ¼ 2.0) and hexane (lgP ¼ 3.5) as well as different mixtures of acetonitrile/aqueous buffer solution. Sensitivity, detection limit, linear range and response time were reported for both biosensors in both media. The performance of the biosensors in the analysis of glucose and phenols in real samples was successful.

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“…Diferentes sistemas bioanalíticos basados en enzimas han sido desarrollados para ser usados en medios orgánicos, generalmente electrodos enzimáticos (87,88 La idea de que las enzimas pueden retener su actividad en una amplia variedad de medios orgánicos está actualmente aceptada (93)(94)(95). Sin embargo, en todos los casos mencionados, las enzimas permanecen activas en función de la fina capa de agua que las rodea y estabiliza.…”

Section: Inmunoensayos Y Medios Orgánicosunclassified

Inmunoensayos en medios orgánicos para el análisis en linea de plaguicidas

Villegas¹

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CERTIFICAN:Que las investigaciones recogidas en la presente memoria "Inmunoensayos en medios orgánicos para el análisis en línea de plaguicidas", que presenta D. José Penalva Villegas, Licenciado en Ciencias Químicas, han sido realizadas bajo nuestra dirección y cumplen con las normas en vigor en cuanto a calidad científica y volumen de trabajo. Por ello, autorizamos su presentación para ser defendida como Tesis Doctoral. Karl Gustav Jung Agradecimientos Agradecimientos Agradecimientos AgradecimientosEn el momento de escribir la Tesis Doctoral en la que se recogen los resultados obtenidos a lo largo de varios años, es imposible sustraerse a los recuerdos y personas que han influido enormemente en este periodo. Por tanto, me gustaría expresar mi más sincera gratitud a:Mis directores, Ángel Maquieira Catalá y Rosa Puchades Pla, quienes además de aceptar la dirección de esta Tesis, me han apoyado en todo momento.A todos mis compañeros y amigos: Miguel Ángel, Sergi, José Antonio, Paqui, Patricia, Lucía, Paloma, Fernando G., que han sabido crear un ambiente de trabajo muy agradable a lo largo de estos años.A

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Investigation of the effects of polar organic solvents on the activity of tyrosinase entrapped in a poly(ester-sulphonic acid) polymer

Cited by 34 publications

References 16 publications

Enzyme-based electrodes for environmental monitoring applications

Enzyme-based electrodes for environmental monitoring applications

Biosensors Based on Poly(vinylimidazole) Microparticles

Inmunoensayos en medios orgánicos para el análisis en linea de plaguicidas

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