Investigation of the genetic effects of drugs and other biologically active compounds in tests for mutagenesis and DNA-damaging action

The aim of this study was to assess the applicability of the bacterial lux biosensors for genotoxicological studies. Biosensors are the strains of E. coli MG1655 carrying a recombinant plasmid with the lux operon of the luminescent bacterium P. luminescens fused with the promoters of inducible genes: recA, colD, alkA, soxS, and katG. The genotoxicity of forty-seven chemical compounds was tested on a set of three biosensors pSoxS-lux, pKatG-lux and pColD-lux, which allowed us to estimate the oxidative and DNA-damaging activity of the analyzed drugs. The comparison of the results with the data on the mutagenic activity of these drugs from the Ames test showed a complete coincidence of the results for the 42 substances. First, using lux biosensors, we have described the enhancing effect of the heavy non-radioactive isotope of hydrogen deuterium (D2O) on the genotoxicity of chemical compounds as possible mechanisms of this effect. The study of the modifying effect of 29 antioxidants and radioprotectors on the genotoxic effects of chemical agents showed the applicability of a pair of biosensors pSoxS-lux and pKatG-lux for the primary assessment of the potential antioxidant and radioprotective activity of chemical compounds. Thus, the results obtained showed that lux biosensors can be successfully used to identify potential genotoxicants, radioprotectors, antioxidants, and comutagens among chemical compounds, as well as to study the probable mechanism of genotoxic action of test substance.

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Investigation of the genetic effects of drugs and other biologically active compounds in tests for mutagenesis and DNA-damaging action

Cited by 3 publications

References 7 publications

Studying the Mechanism of Dioxidine Genotoxicity Using Lux Biosensors of Esсherichia coli

Studying the Mechanism of Dioxidine Genotoxicity Using Lux Biosensors of Esсherichia coli

Dioxidine induce antibiotic-resistant bacteria

Bacterial Lux Biosensors in Genotoxicological Studies

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