Investigation of the JASMONATE ZIM-DOMAIN Gene Family Reveals the Canonical JA-Signaling Pathway in Pineapple

Abstract

JASMONATE ZIM-DOMAIN (JAZ) proteins are negative regulators of the jasmonate (JA)-signaling pathway and play pivotal roles in plant resistance to biotic and abiotic stresses. Genome-wide identification of JAZ genes has been performed in many plant species. However, systematic information about pineapple (Ananas comosus L. Merr.) JAZ genes (AcJAZs) is still not available. In this study, we identified 14 AcJAZ genes and classified them into five groups along with the Arabidopsis and rice orthologs. The AcJAZ genes have 3–10 exons, and the putative AcJAZ proteins have between two and eight conserved regions, including the TIFY motif and Jas domain. The cis-acting element analysis revealed that the putative promoter regions of AcJAZs contain between three and eight abiotic stress-responsive cis-acting elements. The gene-expression analysis suggested that AcJAZs were expressed differentially during plant development and subjected to regulation by the cold, heat, salt, and osmotic stresses as well as by phytohormones. Moreover, the BiFC analysis of protein interactions among the central JA-signaling regulators showed that AcJAZ4, AcMYC2, AcNINJA, and AcJAM1 could interact with AcJAZ5 and AcJAZ13 in vivo, indicating a canonical JA-signaling pathway in pineapple. These results increase our understanding of the functions of AcJAZs and the responses of the core players in the JA-signaling pathway to abiotic stresses.

Genome-Wide Identification of MYC Transcription Factors and Their Potential Functions in the Growth and Development Regulation of Tree Peony (Paeonia suffruticosa)

“…The TIFY family plays a significant role in regulating various aspects of plant development, abiotic stresses, and phytohormone treatments [38]. In Ananas comosus, JAZ proteins have the capability to bind to MYC transcription factors and recruit TIFY proteins to regulate the growth and development process and abiotic stresses response [39]. These results indicate that PsMYCs might play vital roles in the growth and development regulation of tree peony, while the specific regulatory mechanisms still need to be further studied.…”

Mealybug salivary microbes inhibit induced plant defenses

“…Previous studies suggest that lipoxygenase ( LOX ), 12–oxo–phytodienoic acid reductases ( OPRs ) and jasmonate zim‐domain ( JAZ ) are key JA‐signaling regulators and are associated with JA biosynthesis 44–46 . The pathogenesis‐associated genes ( PRs ), phenylalanine ammonia‐lyase ( PAL ) and 4‐coumarate:CoA ligase ( 4CL ) contribute to SA biosynthesis 47,48 .…”

“…Previous studies suggest that lipoxygenase (LOX), 12-oxo-phytodienoic acid reductases (OPRs) and jasmonate zim-domain (JAZ) are key JA-signaling regulators and are associated with JA biosynthesis. [44][45][46] The pathogenesis-associated genes (PRs), phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL) contribute to SA biosynthesis. 47,48 To evaluate whether saliva microbes of P. solenopsis affect plant defense responses, the expression of marker genes associated with the JA and SA signaling pathway was investigated, including LOX1, OPR3, JAZ1, PR1, PAL and 4CL.…”

Editorial to the Special Issue “Eco-Physiological and Molecular Basis of Stress Tolerance in Plants”

“…In this regard, three papers are included in this special issue. The first one was published by Ye and his colleagues, who characterised the functionality of the JASMONATE ZIM-DOMAIN (JAZ) gene family in pineapple [ 12 ]. JAZ proteins are negative regulators of jasmonate signalling in response to abiotic and biotic stress in plants.…”

“…They identified 14 JAZ genes in pineapple, which were highly upregulated and expressed during plant development in response to different abiotic stress conditions including salinity, high and low temperature, osmotic stress, and phytohormone application. Moreover, a bimolecular fluorescence complementation analysis showed that these JAZ proteins could interact with other stress regulatory proteins, such as MYC2, NINJA, and JAM1 to regulate abiotic stress tolerance in plants [ 12 ]. Thus, targeting JAZ proteins could regulate abiotic stress tolerance in plants.…”