Investigation of the mechanisms by which “enhancing” antiserum prevents induction of delayed hypersensitivity to protein antigens in mice

Crowle, Alfred J.; Hu, Cai

doi:10.1016/0021-8707(69)90064-1

Cited by 30 publications

(7 citation statements)

References 19 publications

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“…Existence of DTH-memory, manifested by an accelerated and enhanced DTH response in primed animals upon the reinjection of an antigen, has been described for several antigens (2,3,4,11,18,19). In an earlier paper of this series, we showed that the DTH response to ovalbumin (OA) in mice was also accelerated and enhanced by priming with a low dose of urea-denatured ovalbumin (UD-OA) (9).…”

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confidence: 99%

Regulatory Mechanism of Delayed‐Type Hypersensitivity in Mice: III. In Vitro Analysis of Memory T Cells Involved in Augmentation of DTH Responses

Tamura

Kojima

Egashira

1981

Microbiology and Immunology

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The memory of delayed-type hypersensitivity (DTH), manifested by the augmented responsiveness upon challenge with alum-absorbed ovalbumin (GA), was induced in mice primed 7 days, 21 days, or 90 days previously with I Ilg of reduced and alkylated GA. The memory cells involved in the augmentation of DTH responses were analyzed in the in vitro induction system of T cells which mediate DTH against GA. Spleen cells from the primed mice generated DTH-effector T cells (DTH-Te) in a significantly accelerated fashion, compared with unprimed spleen cells, when cultured with GA. The accelerated generation of DTH-Te in vitro was induced antigen specifically and was dependent on a certain'T cell population in the primed spleen. The T cell population was found in the spleen of primed mice for at least 3 months after priming, corresponding to the persistence of DTH-memory in vivo. Moreover, it was fractionated in the high-density layer by discontinuous bovine serum albumin gradient centrifugation. The high-density cell population decreased in density with increase in the time of culture and developed into DTH-Te, which were separated in the low-density layer on day 4 of culture. These results indicate that the T cells involved in the accelerated generation of DTH-Te in vitro are long-lived DTH-memory T cells, which are probably precursor cells, capable of differentiating into DTH-Te upon challenge with the antigen.An investigation of the properties of immunological memory for a delayedtype hypersensitivity (DTH) response is important for an understanding of the regulatory mechanism of the response. Existence of DTH-memory, manifested by an accelerated and enhanced DTH response in primed animals upon the reinjection of an antigen, has been described for several antigens (2,3,4, 11,18,19). In an earlier paper of this series, we showed that the DTH response to ovalbumin (OA) in mice was also accelerated and enhanced by priming with a low dose of urea-denatured ovalbumin (UD-OA) (9). Splenic T cells from the primed mice

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confidence: 99%

Regulatory Mechanism of Delayed‐Type Hypersensitivity in Mice: III. In Vitro Analysis of Memory T Cells Involved in Augmentation of DTH Responses

Tamura

Kojima

Egashira

1981

Microbiology and Immunology

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“…This phenom enon, described by Jadassohn in 1923, has often been referred to as 'hardening' of the skin. Unresponsiveness to contact sensitizers can be induced by buccal administration of antigen [Lowney, 1968], by antigen feeding [Chase, 1946], by intravenous injection of antigen [Frey et al, 1964;A xelrad, 1968;Axelrad and R owley, 1968; deW it and Bleumink, 1970] or by passive transfer of antibody [Crowle and Hu, 1965;Axelrad, 1968;Crowle and Hu, 1969]. De layed hypersensitivity may be suppressed in the presence of an unaffected formation of humoral antibody, a phenomenon which has been referred to as immune deviation [Asherson and Stone, 1965].…”

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confidence: 99%

The Influence of Antibody on the Induction and Elicitation of Allergic Contact Dermatitis

Roupe¹,

Strannegård²

1972

Int Arch Allergy Immunol

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The development of contact hypersensitivity after epicutaneous application of dinitrochlorobenzene in guinea pigs was suppressed by antidinitrophenyl γ1 and γ2 antibody which was injected locally at the sensitization sites. Some results suggested that small amounts of antibody may enhance rather than suppress the induction of allergic contact dermatitis. The elicitation of the delayed epidermal reactions was suppressed by antibody. These results indicate that the final outcome of sensitization to a simple chemical is dependent on several possible effects of preexistent or newly formed antibodies. They suggest that a lowered skin reactivity to an allergen in allergic contact dermatitis may be due to antibody-mediated depression of delayed hypersensitivity.

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“…Although, with the aid of special techniques, the capacity of mice to express certain cellular immunities, including transplantation immunity, in terms of delayed skin reactions has been demonstrated by a few investigators (12)(13)(14)(15), most students of delayed hypersensitivity still regard these animals as unfavorable subjects for study (6,16).…”

Section: Received For Publication 29 September 1969)mentioning

confidence: 99%

An Analysis of the Genetic Requirements for Delayed Cutaneous Hypersensitivity Reactions to Transplantation Antigens in Mice

Streilein

Billingham

1970

The Journal of Experimental Medicine

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The experiments reported herein provide ample evidence that mice, like most other mammalian species, are capable of displaying readily observable and reproducible delayed cutaneous hypersensitivity reactions indicative of transplantation immunity. By employing a variety of genetically defined strains, it has been shown that a genetic requirement for the development of a positive normal lymphocyte transfer reaction in mice is a difference between host and cell donor at the H-2 locus. By contrast, the immune lymphocyte transfer reaction consistently reflected the full range of histoincompatibility, both inclusive and exclusive of the H-2. It was incidentally discovered that erythema regularly accompanied delayed cutaneous reactions in the skins of female mice, whereas no local redness accompanied their counterparts in male skins. The influence of cutaneous erythema on the scoring of delayed skin reactions is discussed.

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Investigation of the mechanisms by which “enhancing” antiserum prevents induction of delayed hypersensitivity to protein antigens in mice

Cited by 30 publications

References 19 publications

Regulatory Mechanism of Delayed‐Type Hypersensitivity in Mice: III. In Vitro Analysis of Memory T Cells Involved in Augmentation of DTH Responses

Regulatory Mechanism of Delayed‐Type Hypersensitivity in Mice: III. In Vitro Analysis of Memory T Cells Involved in Augmentation of DTH Responses

The Influence of Antibody on the Induction and Elicitation of Allergic Contact Dermatitis

An Analysis of the Genetic Requirements for Delayed Cutaneous Hypersensitivity Reactions to Transplantation Antigens in Mice

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