Investigation of the oxidative stress condition for occupational exposure to methyl methacrylate

Abstract

The purpose of this study is the evaluation of possible toxicological effects on dental personnel, who are exposed to the production materials of prostheses during their entire working life. In this study, the level of methyl methacrylate (MMA) in ambient air was measured as an indicator of external exposure on the personnel. In order to evaluate the possible toxic effects of oxidative stress, which is thought to play an important role in the formation process of many diseases such as cancer, blood samples of volunteers were examined via the ultraviolet-spectroscopic determination of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase antioxidant enzyme activities, comparing occupationally MMA-exposed ( n = 69) and control ( n = 67) groups. In these groups, malondialdehyde (MDA) and glutathione levels were also determined by the same method. The results showed that SOD activity and MDA and glutathione levels were significantly higher in the exposed group when compared with the control group ( p < 0.001). A significant decrease was determined in the glutathione peroxidase activities of the exposed group ( p < 0.05). No significant difference in CAT activities between the groups has been found. When the results obtained in the study are considered, it is suggested that an adaptive response might be developed by dental technicians against low-level MMA exposure.

Inflammation in the lungs of mice due to methyl methacrylate exposure

“…Furthermore, there were no differences in the expression levels of IL-8 in the control and treatment groups. However, these results cannot be extrapolated to those of chronic exposure [15]. At low levels of MMA exposure, an adaptive response can occur [16].…”

Cytogenetic Biomonitoring of Dental Technicians: A Cross‐Sectional Study

“…Several studies have demonstrated the genotoxic potential of dental laboratory material used by dental technicians . A survey by Burgaz et al compared dental technicians exposed to metal alloys to a control group.…”

“…Several studies have demonstrated the genotoxic potential of dental laboratory material used by dental technicians. 2,7,24 A survey by Burgaz et al 7 compared dental technicians exposed to metal alloys to a control group. Their data revealed that in vivo exposure to chromium, nickel, and cobalt metals is evident and contributes to the genotoxic damage in lymphocytes and exfoliated nasal cells; however, which compound(s) are responsible for the genotoxic damage could not be determined.…”

“…The average doses of cobalt, nickel, and chromium among technicians were about 66, 11, and 1900 times higher than those of the control group. Nevertheless, Soykut et al investigated the effects of low‐level MMA exposure on the antioxidant defense system and concluded that superoxide dismutase and malondialdehyde activities were significantly higher in blood samples of dental technicians when compared to matched controls with no MMA exposure.…”

Genotoxicity Induced by Dental Materials: A Comprehensive Review

“…Results obtained from murine macrophages in vitro revealed that BISGMA exhibited genotoxicity in a dose-related fashion as a result of increasing numbers of DNA strand breaks and micronuclei formation (23). When people were occupationally exposed to methyl methacrylate, superoxide dismutase activity, malondealdehyde, and glutathione levels were significantly higher in blood samples of dental technicians when compared to matched controls (24). Analogous products, such as 2,2-bis [4-(acryloxypropoxy)phenyl]propane also increased micronuclei frequency in human gingival fibroblasts in vitro (25).…”