Investigation of the possibility of culturing aerobic yeast with oxygen nanobubble addition and evaluation of the results of batch and semi-batch cultures of Saccharomyces cerevisiae

Sobieszuk, Paweł; Strzyżewska, Alicja; Ulatowski, Karol

doi:10.1016/j.cep.2020.108247

Cited by 14 publications

(18 citation statements)

References 22 publications

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“…In the realm of “nanogasification”, for example, adjusting requisite amounts of NBs allows for regulating the viscosity, density, electrical conductivity, turbidity, and other properties. In turn, this allows us to contemplate the prospect of efficient and effective solvent-design strategies to optimize these NB properties for various applications, for example, in the case of aqueous solvents, “nano-oxygenation” for activated-sludge water treatment, dissolved-air flotation, irrigation and fermentation, suppression of biofouling in dairy and process industries, and powerfully, algal formation in water bodies (given disastrous methane emissions therefrom while increasingly in eutrophic states) (Figure ).…”

Section: Holey Liquids and The “Nanophase”mentioning

confidence: 99%

Sustainable Exploitation and Commercialization of Ultradense Nanobubbles: Reinventing Liquidity

English¹

2022

ACS Sustainable Chem. Eng.

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Section: Holey Liquids and The “Nanophase”mentioning

confidence: 99%

Sustainable Exploitation and Commercialization of Ultradense Nanobubbles: Reinventing Liquidity

English¹

2022

ACS Sustainable Chem. Eng.

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“…In the realm of "nano-gasification", for example, adjusting the requisite amounts of NBs allows for regulating the viscosity, density, electrical conductivity, turbidity, and other properties. In turn, this allows us to contemplate the prospect of efficient and effective solvent-design strategies to optimise these NB properties for various applications (e.g., in the case of aqueous solvents, "nano-oxygenation" for activated-sludge water treatment [82], dissolved-air flotation [83], irrigation and fermentation [84] as well as the suppression of biofouling in the dairy and agricultural industries, and, powerfully, algal formation in water bodies (given the disastrous methane emissions therefrom whilst increasingly in eutrophic states [85]).…”

Section: The Context For Nanobubbles In Environmental Sustainabilitymentioning

confidence: 99%

Environmental Exploration of Ultra-Dense Nanobubbles: Rethinking Sustainability

English

2022

Environments

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Nanobubbles are nanoscopic gaseous domains than can exist on solid surfaces or in bulk liquids. They have attracted significant attention in the last decade due to their long-time (meta)stability and ready potential for real-world applications, especially in environmental engineering and more sustainable ecosystems, water treatment, irrigation, and crop growth. After reviewing important nano-bubble science and activity, with some of the latest promising results in agriculture, we point out important directions in applications of nano-bubble phenomena for boosting sustainability, with viewpoints on how to revolutionise best-practice environmental and green sustainability, taking into account economic drivers and impacts. More specifically, it is pointed out how nanobubbles may be used as delivery vehicles, or “nano-carriers”, for nutrients or other agents to specific targets in a variety of ecosystems of environmental relevance, and how core this is to realising a vision of ultra-dense NBs in shaping a positive and lasting impact on ecosystems and our natural environment.

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“…We used nanobubbles in the Saccharomyces cerevisiae yeast culture in 3 and 5 L vessels. 5 Based on the elemental balance of growth, we deduced that the oxygen enclosed in nanobubbles would not be sufficient to maintain the culture for the typical concentration of nanobubbles generated using the hydrodynamic method. We have carried out a typical batch culture with barbotage aeration, but the inoculum and broth were saturated with oxygen nanobubbles.…”

Section: Introductionmentioning

confidence: 98%

“…The collective findings indicate that contact with a dispersion of nanobubbles of nontoxic gases increases the metabolic rate of microorganisms. [4][5][6]9 Guo et al also suggested that the gas used to generate nanobubbles impacts the bacteria, but more importantly, the sole presence of gas nanobubbles of any gas, even those considered neutral, influences the growth and metabolic rate of bacteria. 4 Results reported in the literature for bacteria and yeast cultures are auspicious from both scientific and industrial points of view as numerous processes are based on microorganism usage, such as organic acid production, wastewater treatment, microalgae cultures for photosynthesis, and organic oil production, among others.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nanobubble Presence on Murine Fibroblasts and Human Leukemia Cell Cultures

et al. 2022

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Nanobubbles can enhance both the proliferation and metabolic activity of microorganisms (mainly bacteria) and the growth of the whole higher organisms such as mice, fish, or plants. The critical fact is that nanobubbles of different gases can affect given cells differently. As animal cell cultures are used in industry and research studies, investigations of their interactions with nanobubbles should be carried out. This study aims to uncover whether the presence of nanobubbles improves the proliferation rate and metabolic activity of L929 fibroblasts and HL60 leukemia cells as exemplary animal cell lines of adherent and non-adherent cells, respectively. The long-term (8-day) cultures of both L929 and HL-60 cells with nanobubble addition to the appropriate medium were carried out. The medium was not exchanged for the whole duration of the culture. Nanobubbles of two gases – oxygen and nitrogen – were dispersed in the appropriate media and then used to culture cells. The density and viability of cells were assessed microscopically while their metabolic activity was determined using PrestoBlue or XTT assays. Additionally, we have performed the analysis of substrate consumption rate during the growth and activity of lactate dehydrogenase. We have shown that nanodispersion of both gases enhances the proliferation rate and metabolic activity of L929. For HL-60 cultures, reference cultures exhibited better viability, cell density, and metabolic activity than those with either oxygen or nitrogen nanobubbles. Obtained results clearly show that nanobubble dispersions of both oxygen and nitrogen positively affect the cultures of L929 while inhibiting the growth of HL-60 cells. We suspect that a similar positive effect would be visible for other adherent cells, similar to L929. Such results are promising for intensifying the growth of animal or human cells in routine cell cultures.

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Investigation of the possibility of culturing aerobic yeast with oxygen nanobubble addition and evaluation of the results of batch and semi-batch cultures of Saccharomyces cerevisiae

Cited by 14 publications

References 22 publications

Sustainable Exploitation and Commercialization of Ultradense Nanobubbles: Reinventing Liquidity

Sustainable Exploitation and Commercialization of Ultradense Nanobubbles: Reinventing Liquidity

Environmental Exploration of Ultra-Dense Nanobubbles: Rethinking Sustainability

Effect of Nanobubble Presence on Murine Fibroblasts and Human Leukemia Cell Cultures

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