Investigation of the Presence of Ornithobacterium rhinotracheale in Chickens in Turkey and Determination of the Seroprevalance of the Infection Using the Enzyme-Linked Immunosorbent Assay

In the present study, lung, trachea and serum samples from broiler flocks slaughtered at an abattoir in Elazig province located in the East of Turkey were examined for the presence of Ornithobacterium rhinotracheale using culture and enzyme-linked immunosorbent assay (ELISA). The identity was latter proved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), western blot analysis, and polymerase chain reaction (PCR) assays. A total of 324 serum and 250 lung and trachea samples were collected from 10 commercially reared chicken flocks showing respiratory manifestations. The samples were obtained from different flocks. The causative agent (ORT) was isolated from trachea (1.5%) of five chickens and from both lung and trachea (0.4%) of only one chicken in the bacteriological examination of tissues. The presence of antibodies against ORT was detected in 33 (10.2%) of the 324 sera by ELISA. A 784 bp fragment of the 16S rRNA gene was amplified using specific primers in the PCR. All ORT isolates that were positive by culture were also detected to be positive by the PCR. SDS-PAGE protein profiles of whole cell extracts showed a high similarity for all the isolates with a major band of the molecular weight of 33 kDa (kiloDalton). Results of Western blot analysis indicate four antigenic fractions predominantly with molecular weights of 33, 42, 52 and 66 kDa.

Section: Discussionsupporting

confidence: 90%

“…Sakai et al (2000) found that 13.5%, 13.9% and 12.7% of the tested sera of broilers, broiler breeders and layers, respectively were positive. Another study showed antibodies in 64.4% of the tested sera of broiler and layer flocks (Turan and Ak, 2002).…”

Section: Discussionmentioning

confidence: 95%

Investigations on ornithobacterium rhinotracheale in broiler flocks in elazig province located in the east of turkey

Ozbey¹,

Öngör²,

Balik³

et al. 2004

“…In the present study, five of the field strains tested were identified as ORT serotype A and only one strain as serotype B. Scientists reported that serotype A was found to have the highest prevalence especially in chickens in other countries as well as in Turkey (Dudouyt et al, 1995;Van Empel et al, 1997;Turan and Ak, 2002). Erganis et al (2002) reported that of the two strains isolated in Turkey, one was serotype B and the other could not be serotyped.…”

Section: Discussionmentioning

confidence: 52%

“…Turkyilmaz (2001) suggested that three isolates were ORT serotype I. Turan and Ak (2002) showed that 90.9% were determined to be serotype A and one strain could not be serotyped. Our results are in agreement with the results reported in other countries and Turkey (Dudouyt et al, 1995;Van Empel et al, 1997;Erganis et al 2002;Turan and Ak, 2002).…”

Section: Discussionmentioning

confidence: 99%

Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey

Ozbey¹,

Ertaş²,

Muz³

2005

Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer . Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a different RAPD profile. Within strains of the same serotype (A), different profiles were found but the strain of serotype (B) had an identical profile as strains of serotype A. This study suggests that more genotypes of ORT strains are present within the same serotype and thus that no relationship exists between the RAPD pattern of ORT and their serotype.

“…The antibiotics of choice are tetracycline, chlortetracycline and amoxiciline. High seroprevalences of O. rhinotracheale infection have been recorded in some countries in Europe and Asia, especially in Germany 26% (Hafez and Sting 1996), in Turkey 62.5% (Turan and Ak 2002) and in Iran 44.2% (Allymehr 2006), mostly in broiler flocks.…”

mentioning

confidence: 99%

Prevalence of etiological agents of selected respiratory infections in chicken and turkey farms in the Czech Republic

Lobová¹,

Kohoutová²,

Molínková³

et al. 2012

ABSTRACT:The causative agents of respiratory diseases of turkeys represent, primarily in fattening farms, a substantial risk of economic and breeding problems. The purpose of this communication is to provide information on the prevalence of respiratory agents of turkeys and chickens in several fattening and production farms in Southern Moravia. This study was focused on pathogens causing bacterial diseases such as Ornithobacteriosis and Mycoplasmosis, as well as viral rhinotracheitis and laryngotracheitis of poultry. The laboratory diagnosis of these diseases has been performed in our institute since January 2008. We examined 249 samples of turkeys and chickens from a single rearing house and six fattening farms in Southern Moravia. The samples were examined using the PCR or RT-PCR method. The typing of isolates of Ornithobacterium rhinotracheale was done using the M13 fingerprinting method. We established the prevalence of pathogens such as Ornithobacterium rhinotracheale (ORT), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), avian metapneumovirus (aMPV) and laryngotracheitis virus (ILT) in selected farms.