Older adults are a vulnerable group and face a series of problems that are preventable by changing their lifestyle, so it is necessary to understand the healthy lifestyle and its related factors in older adults. This study aimed to determine the relationship between healthy lifestyle, self-esteem, and subjective vitality in the older adults of Sirjan. This descriptive-analytical cross-sectional study included 200 older adults referred to community health centers in Sirjan in 2021. Older adults with inclusion criteria were selected by two-stage cluster sampling. Data were collected by demographic information questionnaire, the healthy lifestyle questionnaire for elderly, the Rosenberg self-esteem scale, and the subjective vitality scale. The data were analyzed using SPSS20 and descriptive and inferential statistics (Mann–Whitney U test, Kruskal–Wallis, post hoc tests, and Spearman correlation coefficient). A significance level <0.05 was considered. The study results showed that the mean scores of healthy lifestyle, self-esteem, and subjective vitality were 134.42 ± 13.78, 36.34 ± 4.62, and 21.80 ± 2.85, respectively. We observed a direct and significant relationship between healthy lifestyle, self-esteem, and subjective vitality (
p
<
0.001
). Considering the relationship between healthy lifestyle, self-esteem, and subjective vitality in older adults, managers and planners can help older adults to promote their healthy lifestyles.