Investigation of the Siderophore Production and Associated Heavy Metal Accumulation Potential of Brevibacillus laterosporus 301/İK3-2

Özdemir, F. İnci; AYDIN, Bahadır; Tülek, Ahmet

doi:10.15671/hjbc.1256836

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Pseudomonas species are wellknown for their metabolic diversity and ability to thrive in various environmental conditions while Streptomyces species, known for their proli c production of antibiotics, also play a crucial role in soil health and HM tolerance. Özdemir, Aydın [39] has demonstrated that Pseudomonas can produce siderophores, which are iron-chelating compounds that also bind other HM, reducing their toxicity and facilitating their uptake and sequestration. Lemare, Puja [40] studied Pseudomonas species can biotransform HM into less toxic forms through redox reactions, thereby mitigating metal-induced oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the role of contaminants reshaping the microflora in Zea mays seeds from heavy metal-contaminated and pristine environment

Awais,

Xianga,

Shah

et al. 2024

Preprint

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Heavy metal (HM) contaminants are the emerging driving force for reshaping the microflora of plants by eradicating the non-tolerance and non-resistant microbes via their lethal effects. Seeds serve as a primary source of microbial transmission from one plant generation to the next however, being exposed to metal contamination reshapes their microflora through generations. In this study, the endophytic diversity was compared within Zea mays seeds between three distinct regions in Yunnan province China i.e., HM-contaminated sites (AK) less-contaminated (SD), and a non-contaminated Site (DL). High throughput sequencing technique were used to analyze 492,177 high-quality reads for bacteria and 1,001,229 optimized sequences for fungi. Reads of interest were grouped into operational taxonomic units (OTUs), assigned to microbial genomes and analyzed for taxonomic information, alpha and beta diversity, taxonomic composition, and differential analyses. A higher diversity was recorded in AK samples than in SD and DL. Microbial community structure analysis showed higher diversity and significant fluctuation in specific taxa abundance in the metal-polluted samples exhibiting higher response of microbial flora to HM. In AK samples Gordonia and Burkholderia-Caballeronia-Paraburkholderi, were HM-resistant while Pseudomonas and Streptomyces showed moderate tolerance to HM. Fungal taxa such as Fusarium, Saccharomycopsis, and Lecanicillium also showed metal tolerance. The results revealed the role of HM in reshaping the microflora and their response in maize seeds. While investigating the microbiome from different environments of maize seeds using sequencing technology, the study will help in studying the microbiome shifts under HM conditions aiding better management strategies for reclaiming contaminated soil and crop management.

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Section: Discussionmentioning

confidence: 99%

Unraveling the role of contaminants reshaping the microflora in Zea mays seeds from heavy metal-contaminated and pristine environment

Awais,

Xianga,

Shah

et al. 2024

Preprint

View full text Add to dashboard Cite

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Unraveling the Role of Contaminants Reshaping the Microflora in Zea mays Seeds from Heavy Metal–Contaminated and Pristine Environment

Awais,

Xiang,

Shah

et al. 2024

Microb Ecol

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Heavy metal (HM) contaminants are the emerging driving force for reshaping the microflora of plants by eradicating the non-tolerance and non-resistant microbes via their lethal effects. Seeds served as a prime source of ancestral microbial diversity hereditary transfer from generation to generation. However, the problem arises when they got exposed to metal contamination, does metal pollutant disrupt the delicate balance of microbial communities within seeds and lead to shifts in their microflora across generations. In this study, the endophytic community within Zea mays seeds was compared across three distinct regions in Yunnan province, China: a HM-contaminated site Ayika (AK), less-contaminated site Sanduoduo (SD), and a non-contaminated Site Dali (DL). High-throughput sequencing techniques were employed to analyze the microbial communities. A total of 492,177 high-quality reads for bacterial communities and 1,001,229 optimized sequences for fungal communities were obtained. These sequences were assigned to 502 and 239 operational taxonomic units (OTUs) for bacteria and fungi, respectively. A higher diversity was recorded in AK samples than in SD and DL. Microbial community structure analysis showed higher diversity and significant fluctuation in specific taxa abundance in the metal-polluted samples exhibiting higher response of microbial flora to HM. In AK samples, bacterial genera such as Gordonia and Burkholderia-Caballeronia-Paraburkholderia were dominant, while in SD Pseudomonas and Streptomyces were dominant. Among the fungal taxa, Fusarium , Saccharomycopsis , and Lecanicillium were prevalent in HM-contaminated sites. Our finding revealed the influential effect of HM contaminants on reshaping the seed microbiome of the Zea mays , showing both the resilience of certain important microbial taxa as well the shifts in the diversity in the contaminated and pristine conditions. The knowledge will benefit to develop effective soil remediation, reclamation, and crop management techniques, and eventually assisting in the extenuation of metal pollution's adverse effects on plant health and agricultural productivity.

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Antifungal Potential of Bacillus subtilis subsp. inaquosorum RLS76 for Management of Fusarium Wilt Disease of Bt- Cotton

Raut,

Dalvi,

Rakh

2024

Curr Agri Res Jour

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Fusarium wilt is a destructive disease affecting Bt cotton that is caused by Fusarium oxysporum f. sp. vasinfectum. This seed and soilborne disease pose a significant risk to Bt cotton production worldwide, including India. Therefore, to cope with this problem in an eco-friendly manner, we explored the potentiality of the rhizobacterial isolate possessing antifungal activity as a natural arsenal against the fungal pathogen fusarium wilt of Bt cotton. The rhizobacterial isolate RLS76 inhibited 85.39% of the fungal phytopathogen in the dual culture method. The RLS76 isolate's 16S rRNA partial gene sequence was determined to be Bacillus subtilis subsp. inaquosorum RLS76, with a closest phylogenetic affiliation of 99.53%. The crude extract of Bacillus subtilis subsp. inaquosorum RLS76 demonstrated 34.38% inhibition of the growth of the pathogen after a week of incubation by using an agar well diffusion method. Bacillus subtilis subsp. inaquosorum RLS76 produced volatile organic compounds that inhibited the fungal growth of phytopathogens by 54.84% in a closed environment. Also, GCMS analysis of the crude extract of Bacillus subtilis subsp. inaquosorum RLS76 identified four compounds, namely Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-; Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); 2,5-cyclohexadiene-1,4-dione, 2,5-Dihydroxy-3-methoxy-6-methyl-; 9,12-Octadecadienoic acid (Z,Z)-,phenylmethyl ester. Out of four compounds, three exhibit antimicrobial activity except 2,5-Dihydroxy-3-methoxy-6-methyl. The RLS76 strain of Bacillus subtilis subsp. inaquosorum displayed a transition in hue, shifting from blue to orange, surrounding the colony on CAS medium by producing siderophore qualitatively. The quantitative siderophore produced in the crude extract was 86.06 SU after 60-72 h of incubation. The RLS76 strain of Bacillus subtilis subsp. inaquosorum also showed the ability to produce a proteolytic enzyme outside the cell. This enzyme caused a noticeable clear zone around the colony when the bacteria were grown on a skim-milk agar plate. The Bacillus subtilis subsp. inaquosorum RLS76 also exhibited phosphate solubilization activity with the clear halo around the colonies on NBRIP medium. All these results suggest that Bacillus subtilis subsp. inaquosorum RLS76 exhibits good biocontrol as well as a plant growth promoting candidate against the phytopathogen of Fusarium wilt of Bt cotton.

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Investigation of the Siderophore Production and Associated Heavy Metal Accumulation Potential of Brevibacillus laterosporus 301/İK3-2

Cited by 3 publications

References 21 publications

Unraveling the role of contaminants reshaping the microflora in Zea mays seeds from heavy metal-contaminated and pristine environment

Unraveling the role of contaminants reshaping the microflora in Zea mays seeds from heavy metal-contaminated and pristine environment

Unraveling the Role of Contaminants Reshaping the Microflora in Zea mays Seeds from Heavy Metal–Contaminated and Pristine Environment

Antifungal Potential of Bacillus subtilis subsp. inaquosorum RLS76 for Management of Fusarium Wilt Disease of Bt- Cotton

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