Investigation of the use of rolling circle amplification for the detection of GM food

Pang, Susan; Qureshi, Fizza; Shanahan, Della; Harris, Neil

doi:10.1007/s00217-006-0382-1

Cited by 15 publications

(3 citation statements)

References 27 publications

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“…In recent years, novel techniques such as digital PCR, isothermal nucleic acid amplification, and gene chips have been used for transgene detection [ 16 – 18 ]. Particularly, rapid progress in isothermal nucleic acid amplification technologies, including loop-mediated isothermal amplification [ 19 ], recombinase polymerase amplification [ 20 ], and rolling circle amplification [ 21 ], have provided alternatives to conventional PCR, thereby enabling rapid on-site screening and detection [ 22 , 23 ]. However, conventional and real-time fluorescence PCR remain the gold standard in transgene detection [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

GMOIT: a tool for effective screening of genetically modified crops

Zhou,

Liu,

Liang

et al. 2024

BMC Plant Biol

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Background Advancement in agricultural biotechnology has resulted in increasing numbers of commercial varieties of genetically modified (GM) crops worldwide. Though several databases on GM crops are available, these databases generally focus on collecting and providing information on transgenic crops rather than on screening strategies. To overcome this, we constructed a novel tool named, Genetically Modified Organisms Identification Tool (GMOIT), designed to integrate basic and genetic information on genetic modification events and detection methods. Results At present, data for each element from 118 independent genetic modification events in soybean, maize, canola, and rice were included in the database. Particularly, GMOIT allows users to customize assay ranges and thus obtain the corresponding optimized screening strategies using common elements or specific locations as the detection targets with high flexibility. Using the 118 genetic modification events currently included in GMOIT as the range and algorithm selection results, a “6 + 4” protocol (six exogenous elements and four endogenous reference genes as the detection targets) covering 108 events for the four crops was established. Plasmids pGMOIT-1 and pGMOIT-2 were constructed as positive controls or calibrators in qualitative and quantitative transgene detection. Conclusions Our study provides a simple, practical tool for selecting, detecting, and screening strategies for a sustainable and efficient application of genetic modification.

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Section: Discussionmentioning

confidence: 99%

GMOIT: a tool for effective screening of genetically modified crops

Zhou,

Liu,

Liang

et al. 2024

BMC Plant Biol

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“…Several studies evaluating PCR, real-time PCR, LAMP, and HRCA for the detection of GMOs in feed have been published in recent years. ,− Each study used a single amplification method and different types of samples. In conventional PCR and real-time PCR, the LOD of RRS-specific detection was reported with values of 40 and 20.5 copies, respectively .…”

Section: Discussionmentioning

confidence: 99%

Comparison of Three DNA Extraction Methods for Feed Products and Four Amplification Methods for the 5′-Junction Fragment of Roundup Ready Soybean

Wang

Teng

Tian

et al. 2012

J. Agric. Food Chem.

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Three methods of DNA extraction from feed products and four detection methods for the 5'-junction fragment of genetically modified (GM) Roundup Ready soybean (RRS) were compared and evaluated. The DNA extraction methods, including cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and guanidine hydrochloride (Kit), were assessed for their yields and purity of DNA, extraction time, and reagent cost. The DNA yields of CTAB, SDS, and Kit were 52-694, 164-1750 and 23-105 ng/mg sample, and their extraction time was 2.5-3, 2-2.5, and 1.5-2 h with reagent cost about US dollar 0.24, 0.13, and 1.9 per extraction, respectively. The SDS method was generally well suited to all kinds of feed matrices tested. The limits of detection for the four amplification protocols, including loop-mediated isothermal amplification (LAMP), hyperbranched rolling circle amplification (HRCA), conventional polymerase chain reaction (PCR), and real-time PCR, were 48.5, 4.85, 485, and 9 copies of the pTLH10 plasmid, respectively. The ranked results of the four detection methods were based on multiattribute utility theory as follows (from best to worse): HRCA, LAMP, PCR, and real-time PCR. This comparative evaluation was specifically useful for selection of a highly efficient DNA extraction or amplification method for detecting different GM ingredients.

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“…Pang et al . [ 19 ] investigated the use of rolling circle amplification for the detection of genetically modified food. Here a pre-amplification PCR step was performed prior to the ligation reaction, rolling circle amplification and subsequent PCR amplification.…”

Section: Introductionmentioning

confidence: 99%

Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction

Prins¹,

Dijk²,

Beenen³

et al. 2008

BMC Genomics

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Background: To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a limited extent for EU-nonauthorised GMOs (NAGs). In the last decade the diversity of genetically modified (GM) ingredients in food and feed has increased significantly. As a result of this increase GMO laboratories currently need to apply many different methods to establish to potential presence of NAGs in raw materials and complex derived products.

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Investigation of the use of rolling circle amplification for the detection of GM food

Cited by 15 publications

References 27 publications

GMOIT: a tool for effective screening of genetically modified crops

GMOIT: a tool for effective screening of genetically modified crops

Comparison of Three DNA Extraction Methods for Feed Products and Four Amplification Methods for the 5′-Junction Fragment of Roundup Ready Soybean

Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction

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