Investigation of Turbulent-Mixing-Layer Flow with Polymer Additives and Bubble Injection by Particle Image Velocimetry

Guo, Fei; Chen, Bin; Guo, Liejin; Zhang, Ximin

doi:10.1252/jcej.09we232

Cited by 1 publication

(1 citation statement)

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“…Our previous research has also compared the removal efficiency of the antibiotic by an activated sludge treatment and an algal treatment 31 . The results indicated that the green algae C. pyrenoidosa performed a satisfactory growth ability under the impact of ceftazidime.…”

Section: Introductionmentioning

confidence: 99%

Enhancement by the artificial controlled culture for the algal treatment of antibiotic ceftazidime: a three-step response performance and high-removal efficiency

Feng

Guo

et al. 2015

RSC Adv.

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The improved activity of the alga is critical in the biological enhanced treatment to remove contamination. Organisms usually perform compensatory response resulting from an unfavorable condition. This work investigated the effect of the artificial controlled culture before the treatment, which provoked the possible algal compensatory response and helped the algae to perform a better removal capability on the antibiotic ceftazidime in the subsequent treatment process. The removal efficiency could be improved up to 99.15% in 6 h when the algae was under the artificial controlled light conditions before the treatment process. Additionally, higher removal efficiency (98.57% and 99.98%) was obtained after the artificial control on N and P, respectively. It suggests that the algae displayed a sequence of response during the exposure to the antibiotic after the artificial controlled culture in three steps: compensatory response, adsorption-consumption acceleration and acclimation. It might be the first time that the artificial conditions changing were controlled to improve the removal efficiency. Our work pointed out a new method for biological enhancement technologies in the antibiotic wastewater treatment. 6 investment holding group CO., LTD. Methanol and acetonitrile were HPLC grade.Other chemicals and reagents were analytical grade. Algal culturesThe green algae C. pyrenoidosa (FACHB-1220) which purchased from the Wuhan Hydrobiology Institute of Chinese Academy of Sciences, was pre-cultured in BG-11 media at 25 ± 1 • C and 4000 lux illumination (40 µmol photons m -2 s -1 ) with a light: dark interval of 12 h: 12 h. The algae was cultured for normally to reach the logarithmic growth phase in 3 d and prepared for the subsequent experiments. The experiment had three replications per treatment. Ceftazidime analysisCeftazidime was analyzed using a high-performance liquid chromatography (LC-10A, Shimadzu) apparatus coupled with UV detector. Stock solutions (1 g/L) of ceftazidime were dissolved in BG-11 media. The working solutions (40 mg/L) used in the treatment were then diluted from stock solutions. Antibiotic samples were separated and determined with an Inertsil ODS column (4.6 mm × 150 mm, 5 µm).The mobile phase of ceftazidime was acetonitrile-pH 3.9 phosphate buffer (volume ratio was 7:93). The flow rate was 1.0 mL/min with 20 ± 1.0 • C. The wavelength of the UV detector was 255 nm. Quantitation was performed using external standards and was based on peak areas. Experimental set-upIn the preliminary experiment, the removal rates of the target antibiotic tceftazidime by a dead and living algal cells were evaluated. We also evaluated the concentration

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Section: Introductionmentioning

confidence: 99%